Anti-MEK3 + MEK6 antibody [EPR17345] (ab181555)

Rabbit Recombinant Monoclonal MEK3 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-MEK3 + MEK6 antibody [EPR17345] (AB181555), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	ICC/IF	IHC-P
Human	Tested	Tested	Tested	Tested
Mouse	Expected	Tested	Expected	Tested
Rat	Expected	Tested	Expected	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/140	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/2000	Notes -
Species Human	Dilution info 1/2000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/100	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/100	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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Target data

See full target information MAP2K3

Function

Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14.

Additional Targets

MAP2K6

Alternative names

MEK3, MKK3, PRKMK3, SKK2, MAP2K3, Dual specificity mitogen-activated protein kinase kinase 3, MAP kinase kinase 3, MAPKK 3, MAPK/ERK kinase 3, Stress-activated protein kinase kinase 2, MEK 3, SAPK kinase 2, SAPKK-2, SAPKK2

Recommended products

Rabbit Recombinant Monoclonal MEK3 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR17345
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

MEK3 and MEK6 also known as MAP2K3 and MAP2K6 are dual-specificity protein kinases. MEK3 has a mass of approximately 39 kDa while MEK6 is around 37 kDa. They are both part of the MAP kinase family and mainly expressed in tissues like the skeletal muscle liver and heart. These kinases play a role in phosphorylating and activating p38 MAP kinases which are important for intracellular signaling.

Biological function summary

MEK3 and MEK6 are key components of the MAP kinase signaling pathway. These kinases participate in response networks that regulate stress-induced signaling and inflammatory responses. Although not explicitly forming a classic complex they interact with substrates like p38 MAPK to exert their effects. MEK3 and MEK6 contribute to cellular processes including differentiation apoptosis and gene expression.

Pathways

MEK3 and MEK6 are integral to the p38 MAPK pathway and the stress-activated protein kinase (SAPK) pathways. These pathways facilitate the cellular response to external stress hypoxia and inflammatory cytokines. Their activity is closely related to other MAP kinases like JNK in propagating signals that control cellular stress responses. Their activation mechanism involves sequential phosphorylation events that link initial stimuli to specific cellular reactions.

Associated diseases and disorders

MEK3 and MEK6 are implicated in inflammatory diseases and certain types of cancer. For instance abnormal activity of these kinases is observed in rheumatoid arthritis due to their role in inflammation and cellular differentiation. They also have links to cancers like melanoma through their regulation of apoptosis and cell proliferation. The dysregulation of their network with p38 MAPK and JNK can contribute to the pathology of these conditions making them potential targets for therapeutic interventions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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10 product images

Western blot - Anti-MEK3 + MEK6 antibody [EPR17345] (ab181555)
Based on the sequence analysis, ab181555 recognizes 3 isoforms of MEK3 ranging from 36kDa to 40KDa. The multi-bands should be MEK3 isoforms and MEK6.
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-MEK3 + MEK6 antibody [EPR17345] (ab181555) at 1/50000 dilution
Lane 1: HepG2 (Human liver hepatocellular carcinoma) whole cell lysates at 10 µg
Lane 2: Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates at 10 µg
Lane 3: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates at 10 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 39 kDa, 46 kDa
Observed band size: 34 kDa, 37 kDa, 39 kDa, 51 kDa, 55 kDa
Immunocytochemistry/ Immunofluorescence - Anti-MEK3 + MEK6 antibody [EPR17345] (ab181555)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling MEK3 + MEK6 with ab181555 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/400 dilution (green). Cytoplasm and nuclear staining on HeLa cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:-
1. ab181555 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.
Western blot - Anti-MEK3 + MEK6 antibody [EPR17345] (ab181555)
Recombinant protein covers 139aa-334aa with a His tag (22kDa).
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-MEK3 + MEK6 antibody [EPR17345] (ab181555) at 1/10000 dilution
All lanes: Human MEK6 recombinant protein fragment at 10 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 39 kDa
Observed band size: 22 kDa
Western blot - Anti-MEK3 + MEK6 antibody [EPR17345] (ab181555)
Based on the sequence analysis, ab181555 recognizes 3 isoforms of MEK3 ranging from 36kDa to 40KDa. The multi-bands should be MEK3 isoforms and MEK6.
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-MEK3 + MEK6 antibody [EPR17345] (ab181555) at 1/5000 dilution
Lane 1: Rat liver lysates at 10 µg
Lane 2: Mouse liver lysates at 10 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 39 kDa
Observed band size: 34 kDa, 37 kDa, 39 kDa
Western blot - Anti-MEK3 + MEK6 antibody [EPR17345] (ab181555)
Based on the sequence analysis, ab181555 recognizes 3 isoforms of MEK3 ranging from 36kDa to 40KDa. The multi-bands should be MEK3 isoforms and MEK6.
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-MEK3 + MEK6 antibody [EPR17345] (ab181555) at 1/2000 dilution
Lane 1: Mouse heart lysates at 10 µg
Lane 2: Mouse kidney lysates at 10 µg
Lane 3: Rat heart lysates at 10 µg
Lane 4: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates at 10 µg
Lane 5: NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates at 10 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 39 kDa
Observed band size: 34 kDa, 37 kDa, 39 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK3 + MEK6 antibody [EPR17345] (ab181555)
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling MEK3 + MEK6 with ab181555 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) ab97051 Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Cytoplasmic staining on Human skeletal muscle is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary ab.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-MEK3 + MEK6 antibody [EPR17345] (ab181555)
MEK3 + MEK6 were immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab181555 at 1/140 dilution. Western blot was performed from the immunoprecipitate using ab181555 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: HeLa whole cell extract. Lane 2: PBS instead of HeLa whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-MEK3 + MEK6 antibody [EPR17345] (ab181555)
Predicted band size: 39 kDa
Observed band size: 34 kDa, 36 kDa, 39 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK3 + MEK6 antibody [EPR17345] (ab181555)
Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling MEK3 + MEK6 with ab181555 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) ab97051 Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Cytoplasmic staining on Mouse skeletal muscle is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary ab.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-MEK3 + MEK6 antibody [EPR17345] (ab181555)
MEK3 + MEK6 were immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab181555 at 1/140 dilution. Western blot was performed from the immunoprecipitate using Anti-MKK6 antibody [EP557Y] ab33866 (Rabbit monoclonal [EP557Y] to MEK6) at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: HeLa whole cell extract. Lane 2: PBS instead of HeLa whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-MEK3 + MEK6 antibody [EPR17345] (ab181555)
Predicted band size: 39 kDa
Observed band size: 36 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK3 + MEK6 antibody [EPR17345] (ab181555)
Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling MEK3 + MEK6 with ab181555 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) ab97051 Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Cytoplasmic staining on Rat skeletal muscle is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary ab.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.