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AB250507

Anti-MEK3 + MEK6 antibody [EPR17345] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal MP2K6 antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

MEK6, MKK6, PRKMK6, SKK3, MAP2K6, Dual specificity mitogen-activated protein kinase kinase 6, MAP kinase kinase 6, MAPKK 6, MAPK/ERK kinase 6, Stress-activated protein kinase kinase 3, MEK 6, SAPK kinase 3, SAPKK-3, SAPKK3

10 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK3 + MEK6 antibody [EPR17345] - BSA and Azide free (AB250507)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK3 + MEK6 antibody [EPR17345] - BSA and Azide free (AB250507)

This data was developed using ab181555, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling MEK3 + MEK6 with ab181555 at 1/100 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Cytoplasmic staining on Human skeletal muscle is observed. Counter stained with Hematoxylin.
Negative control : Used PBS instead of primary ab.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-MEK3 + MEK6 antibody [EPR17345] - BSA and Azide free (AB250507)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MEK3 + MEK6 antibody [EPR17345] - BSA and Azide free (AB250507)

This data was developed using ab181555, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling MEK3 + MEK6 with ab181555 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Cytoplasm and nuclear staining on HeLa cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows : -
1. ab181555 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK3 + MEK6 antibody [EPR17345] - BSA and Azide free (AB250507)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK3 + MEK6 antibody [EPR17345] - BSA and Azide free (AB250507)

This data was developed using ab181555, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling MEK3 + MEK6 with ab181555 at 1/100 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Cytoplasmic staining on Mouse skeletal muscle is observed. Counter stained with Hematoxylin.
Negative control : Used PBS instead of primary ab.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK3 + MEK6 antibody [EPR17345] - BSA and Azide free (AB250507)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK3 + MEK6 antibody [EPR17345] - BSA and Azide free (AB250507)

This data was developed using ab181555, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling MEK3 + MEK6 with ab181555 at 1/100 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. Cytoplasmic staining on Rat skeletal muscle is observed. Counter stained with Hematoxylin.
Negative control : Used PBS instead of primary ab.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Western blot - Anti-MEK3 + MEK6 antibody [EPR17345] - BSA and Azide free (AB250507)
  • WB

Supplier Data

Western blot - Anti-MEK3 + MEK6 antibody [EPR17345] - BSA and Azide free (AB250507)

This data was developed using ab181555, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Based on the sequence analysis, ab181555 recognizes 3 isoforms of MEK3 ranging from 36kDa to 40KDa. The multi-bands should be MEK3 isoforms and MEK6.

All lanes:

Western blot - Anti-MEK3 + MEK6 antibody [EPR17345] (<a href='/en-us/products/primary-antibodies/mek3-mek6-antibody-epr17345-ab181555'>ab181555</a>) at 1/2000 dilution

Lane 1:

Mouse heart lysates at 10 µg

Lane 2:

Mouse kidney lysates at 10 µg

Lane 3:

Rat heart lysates at 10 µg

Lane 4:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates at 10 µg

Lane 5:

NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 39 kDa

Observed band size: 34 kDa,37 kDa,39 kDa

false

Immunoprecipitation - Anti-MEK3 + MEK6 antibody [EPR17345] - BSA and Azide free (AB250507)
  • IP

Lab

Immunoprecipitation - Anti-MEK3 + MEK6 antibody [EPR17345] - BSA and Azide free (AB250507)

This data was developed using ab181555, the same antibody clone in a different buffer formulation.

MEK3 + MEK6 were immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab181555 at 1/140 dilution. Western blot was performed from the immunoprecipitate using ab33866 (Rabbit monoclonal [EP557Y] to MEK6) at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1 : HeLa whole cell extract. Lane 2 : PBS instead of HeLa whole cell extract.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-MEK3 + MEK6 antibody [EPR17345] - BSA and Azide free (ab250507)

Predicted band size: 39 kDa

Observed band size: 36 kDa

false

Immunoprecipitation - Anti-MEK3 + MEK6 antibody [EPR17345] - BSA and Azide free (AB250507)
  • IP

Supplier Data

Immunoprecipitation - Anti-MEK3 + MEK6 antibody [EPR17345] - BSA and Azide free (AB250507)

This data was developed using ab181555, the same antibody clone in a different buffer formulation.

MEK3 + MEK6 were immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab181555 at 1/140 dilution. Western blot was performed from the immunoprecipitate using ab181555 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1 : HeLa whole cell extract. Lane 2 : PBS instead of HeLa whole cell extract.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-MEK3 + MEK6 antibody [EPR17345] (<a href='/en-us/products/primary-antibodies/mek3-mek6-antibody-epr17345-ab181555'>ab181555</a>)

Predicted band size: 39 kDa

Observed band size: 34 kDa,36 kDa,39 kDa

false

Western blot - Anti-MEK3 + MEK6 antibody [EPR17345] - BSA and Azide free (AB250507)
  • WB

Supplier Data

Western blot - Anti-MEK3 + MEK6 antibody [EPR17345] - BSA and Azide free (AB250507)

This data was developed using ab181555, the same antibody clone in a different buffer formulation.

Based on the sequence analysis, ab181555 recognizes 3 isoforms of MEK3 ranging from 36kDa to 40KDa. The multi-bands should be MEK3 isoforms and MEK6.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-MEK3 + MEK6 antibody [EPR17345] (<a href='/en-us/products/primary-antibodies/mek3-mek6-antibody-epr17345-ab181555'>ab181555</a>) at 1/5000 dilution

Lane 1:

Rat liver lysates at 10 µg

Lane 2:

Mouse liver lysates at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 39 kDa

Observed band size: 34 kDa,37 kDa,39 kDa

false

Western blot - Anti-MEK3 + MEK6 antibody [EPR17345] - BSA and Azide free (AB250507)
  • WB

Supplier Data

Western blot - Anti-MEK3 + MEK6 antibody [EPR17345] - BSA and Azide free (AB250507)

This data was developed using ab181555, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Based on the sequence analysis, ab181555 recognizes 3 isoforms of MEK3 ranging from 36kDa to 40KDa. The multi-bands should be MEK3 isoforms and MEK6.

All lanes:

Western blot - Anti-MEK3 + MEK6 antibody [EPR17345] (<a href='/en-us/products/primary-antibodies/mek3-mek6-antibody-epr17345-ab181555'>ab181555</a>) at 1/50000 dilution

Lane 1:

HepG2 (Human liver hepatocellular carcinoma) whole cell lysates at 10 µg

Lane 2:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates at 10 µg

Lane 3:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 39 kDa,46 kDa

Observed band size: 34 kDa,37 kDa,39 kDa,51 kDa,55 kDa

false

Western blot - Anti-MEK3 + MEK6 antibody [EPR17345] - BSA and Azide free (AB250507)
  • WB

Supplier Data

Western blot - Anti-MEK3 + MEK6 antibody [EPR17345] - BSA and Azide free (AB250507)

This data was developed using ab181555, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Recombinant protein covers 139aa-334aa with a His tag (22kDa).

All lanes:

Western blot - Anti-MEK3 + MEK6 antibody [EPR17345] (<a href='/en-us/products/primary-antibodies/mek3-mek6-antibody-epr17345-ab181555'>ab181555</a>) at 1/10000 dilution

All lanes:

Human MEK6 recombinant protein fragment at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 39 kDa

Observed band size: 22 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17345

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IP, IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Product details

ab250507 is the carrier-free version of ab181555.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MEK3 and MEK6 also known as MAP2K3 and MAP2K6 are dual-specificity protein kinases. MEK3 has a mass of approximately 39 kDa while MEK6 is around 37 kDa. They are both part of the MAP kinase family and mainly expressed in tissues like the skeletal muscle liver and heart. These kinases play a role in phosphorylating and activating p38 MAP kinases which are important for intracellular signaling.
Biological function summary

MEK3 and MEK6 are key components of the MAP kinase signaling pathway. These kinases participate in response networks that regulate stress-induced signaling and inflammatory responses. Although not explicitly forming a classic complex they interact with substrates like p38 MAPK to exert their effects. MEK3 and MEK6 contribute to cellular processes including differentiation apoptosis and gene expression.

Pathways

MEK3 and MEK6 are integral to the p38 MAPK pathway and the stress-activated protein kinase (SAPK) pathways. These pathways facilitate the cellular response to external stress hypoxia and inflammatory cytokines. Their activity is closely related to other MAP kinases like JNK in propagating signals that control cellular stress responses. Their activation mechanism involves sequential phosphorylation events that link initial stimuli to specific cellular reactions.

MEK3 and MEK6 are implicated in inflammatory diseases and certain types of cancer. For instance abnormal activity of these kinases is observed in rheumatoid arthritis due to their role in inflammation and cellular differentiation. They also have links to cancers like melanoma through their regulation of apoptosis and cell proliferation. The dysregulation of their network with p38 MAPK and JNK can contribute to the pathology of these conditions making them potential targets for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. With MAP3K3/MKK3, catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinases p38 MAPK11, MAPK12, MAPK13 and MAPK14 and plays an important role in the regulation of cellular responses to cytokines and all kinds of stresses. Especially, MAP2K3/MKK3 and MAP2K6/MKK6 are both essential for the activation of MAPK11 and MAPK13 induced by environmental stress, whereas MAP2K6/MKK6 is the major MAPK11 activator in response to TNF. MAP2K6/MKK6 also phosphorylates and activates PAK6. The p38 MAP kinase signal transduction pathway leads to direct activation of transcription factors. Nuclear targets of p38 MAP kinase include the transcription factors ATF2 and ELK1. Within the p38 MAPK signal transduction pathway, MAP3K6/MKK6 mediates phosphorylation of STAT4 through MAPK14 activation, and is therefore required for STAT4 activation and STAT4-regulated gene expression in response to IL-12 stimulation. The pathway is also crucial for IL-6-induced SOCS3 expression and down-regulation of IL-6-mediated gene induction; and for IFNG-dependent gene transcription. Has a role in osteoclast differentiation through NF-kappa-B transactivation by TNFSF11, and in endochondral ossification and since SOX9 is another likely downstream target of the p38 MAPK pathway. MAP2K6/MKK6 mediates apoptotic cell death in thymocytes. Acts also as a regulator for melanocytes dendricity, through the modulation of Rho family GTPases.
See full target information MAP2K6

Additional targets

MAP2K6
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:15564 PubMed38971897

2024

LILRB4 knockdown inhibits aortic dissection development by regulating pyroptosis and the JAK2/STAT3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jianxian Xiong,Jiayuan Ling,Jie Yan,Yanyu Duan,Junjian Yu,Wentong Li,Wenbo Yu,Jianfeng Gao,Dilin Xie,Ziyou Liu,Yongzhi Deng,Yongling Liao
View all publications
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Product promise

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