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AB290001

Anti-MEK3 (phospho S189) + MEK6 (phospho S207) antibody [EPR24759-32] - BSA and Azide free

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Rabbit Recombinant Monoclonal MEK3 phospho S189 antibody. Carrier free. Suitable for WB, Dot, Flow Cyt (Intra), IP and reacts with Human samples.

View Alternative Names

MEK3, MKK3, PRKMK3, SKK2, MAP2K3, Dual specificity mitogen-activated protein kinase kinase 3, MAP kinase kinase 3, MAPKK 3, MAPK/ERK kinase 3, Stress-activated protein kinase kinase 2, MEK 3, SAPK kinase 2, SAPKK-2, SAPKK2, MEK6, MKK6, PRKMK6, SKK3, MAP2K6, Dual specificity mitogen-activated protein kinase kinase 6, MAP kinase kinase 6, MAPKK 6, MAPK/ERK kinase 6, Stress-activated protein kinase kinase 3, MEK 6, SAPK kinase 3, SAPKK-3, SAPKK3

4 Images
Flow Cytometry (Intracellular) - Anti-MEK3 (phospho S189) + MEK6 (phospho S207) antibody [EPR24759-32] - BSA and Azide free (AB290001)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-MEK3 (phospho S189) + MEK6 (phospho S207) antibody [EPR24759-32] - BSA and Azide free (AB290001)

This data was developed using ab289976, the same antibody clone in a different buffer formulation. Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) starved overnight then treated with 250 ng/ml anisomycin for 30 min (Red) /Untreated control (Green) cells labelling MEK3 (phospho S189) + MEK6 (phospho S207) with ab289976 at 1/50 dilution (1ug) (Red) and Green (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

Immunoprecipitation - Anti-MEK3 (phospho S189) + MEK6 (phospho S207) antibody [EPR24759-32] - BSA and Azide free (AB290001)
  • IP

Supplier Data

Immunoprecipitation - Anti-MEK3 (phospho S189) + MEK6 (phospho S207) antibody [EPR24759-32] - BSA and Azide free (AB290001)

This data was developed using ab289976, the same antibody clone in a different buffer formulation. MEK3 (phospho S189) + MEK6 (phospho S207) was immunoprecipitated from 0.35 mg HeLa starved overnight, then treated with 250ng/ml anisomycin for 30 minutes with ab289976 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab289976 at 1/1000 dilution. Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-MEK3 (phospho S189) + MEK6 (phospho S207) antibody [EPR24759-32] (<a href='/en-us/products/primary-antibodies/mek3-phospho-s189-mek6-phospho-s207-antibody-epr24759-32-ab289976'>ab289976</a>) at 1/30 dilution

Lanes 1 - 2:

HeLa starved overnight, then treated with 250ng/ml anisomycin for 30 minutes whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/en-us/products/primary-antibodies/mek3-phospho-s189-mek6-phospho-s207-antibody-epr24759-32-ab289976'>ab289976</a> in HeLa starved overnight, then treated with 250ng/ml anisomycin for 30 minutes whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

false

Exposure time: 49s

Western blot - Anti-MEK3 (phospho S189) + MEK6 (phospho S207) antibody [EPR24759-32] - BSA and Azide free (AB290001)
  • WB

Supplier Data

Western blot - Anti-MEK3 (phospho S189) + MEK6 (phospho S207) antibody [EPR24759-32] - BSA and Azide free (AB290001)

This data was developed using ab289976, the same antibody clone in a different buffer formulation. Loading controls used in this western blot are anti-MEK3 antibody [EPR17345] (ab195037), anti-MEK6 antibody [EP557Y] (ab33866) and GAPDH RabMAb(ab181602) . Alkaline phosphatase was used to treated membrane. Blocking Buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-MEK3 (phospho S189) + MEK6 (phospho S207) antibody [EPR24759-32] (<a href='/en-us/products/primary-antibodies/mek3-phospho-s189-mek6-phospho-s207-antibody-epr24759-32-ab289976'>ab289976</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) starved overnight, whole cell lysate

Lane 2:

HeLa starved overnight, then treated with /ml anisomycin for 30 minutes, whole cell lysate 20 (untreated membrane)

Lane 3:

HeLa starved overnight, then treated with /ml anisomycin for 30 minutes, whole cell lysate 20 (phosphatase treated membrane)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 36 kDa

false

Exposure time: 3min

Dot Blot - Anti-MEK3 (phospho S189) + MEK6 (phospho S207) antibody [EPR24759-32] - BSA and Azide free (AB290001)
  • Dot

Supplier Data

Dot Blot - Anti-MEK3 (phospho S189) + MEK6 (phospho S207) antibody [EPR24759-32] - BSA and Azide free (AB290001)

This data was developed using ab289976, the same antibody clone in a different buffer formulation.

Concentration of ab289976 : 1/1000 dilution (0.572 μg/ml)

Secondary ab : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051), 1/100000 dilution

Blocking/diluting buffer and concentration : 5% NFDM/TBST

Lane 1 : peptide containing phospho S189 of MEK3 (P46734-2)/phospho S207 of MEK6 (P52564-1)

Lane 2 : non-phospho peptide of the corresponding region

Exposure time : 3 minutes

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24759-32

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra), IP, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

AB279860

Phospho-MEK3 (S189) ELISA Kit

Sandwich ELISA - Phospho-MEK3 (S189) ELISA Kit (AB279860)

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Primary Antibodies

AB178876

Anti-MEK1 + MEK2 antibody [EPR16667]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 + MEK2 antibody [EPR16667] (AB178876)

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Primary Antibodies

AB4759

Anti-MEK3 (phospho S189/T193) + MEK6 (phospho S207/T211) antibody

Western blot - Anti-MEK3 (phospho S189/T193) + MEK6 (phospho S207/T211) antibody (AB4759)

  • 0
  • 0
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

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  • 15
Primary Antibodies

AB184699

Anti-ERK1 + ERK2 antibody [EPR17526]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-ERK1 + ERK2 antibody [EPR17526] (AB184699)

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  • 1
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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MEK3 also known as MAP2K3 is a kinase that plays a significant mechanical role within cells. It is part of the MAP kinase family sharing functions with enzymes like MEK6. MEK3 has a molecular mass of approximately 38 kDa and is localized in cytoplasmic regions of various cell types. This protein is expressed in a wide range of tissues indicating its diverse functional roles across different biological systems.
Biological function summary

MEK3 is involved in the phosphorylation cascade within the mitogen-activated protein kinase (MAPK) pathway. It often exists as part of a complex working alongside other kinases to activate p38 MAPK which then regulates gene expression. The involvement of MEK3 in this signaling cascade influences processes such as cellular stress response and inflammation. Its ability to modulate these processes makes MEK3 an important component in maintaining cellular homeostasis.

Pathways

MEK3 functions as an essential link within the stress-activated pathways like the MAPK signaling pathway. It phosphorylates and activates the p38 MAPK pathway therefore regulating activities related to cellular stress and inflammatory responses. MEK3 works in conjunction with related kinases such as MEK6 to propagate the MAPK signaling cascade further emphasizing its important role in these pathways.

MEK3 has a documented involvement in inflammatory conditions and cancer. Its role in activating the p38 MAPK pathway connects it to inflammatory diseases where dysregulated signaling may lead to excessive inflammation. Furthermore aberrant MEK3 activity is associated with certain cancers as it affects cell proliferation and survival. MEK3's interactions with other kinases like MEK6 underline its involvement in these pathological processes making it a potential target for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14.
See full target information MAP2K3 phospho S189

Additional targets

MAP2K6 phospho S207
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com