AB4759

Anti-MEK3 (phospho S189/T193) + MEK6 (phospho S207/T211) antibody

Name: Phospho Anti-MEK3 (S189/T193) + MEK6 (S207/T211) antibody (ab4759)
Brand: Abcam Limited
SKU: AB4759
Price: 595 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal MEK3 phospho S189 + T193 antibody. Suitable for WB and reacts with Synthetic peptide - Human, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human MAP2K3 phospho S189 + T193.

View Alternative Names

MEK3, MKK3, PRKMK3, SKK2, Dual specificity mitogen-activated protein kinase kinase 3, MAP kinase kinase 3, MAPKK 3, MAPK/ERK kinase 3, Stress-activated protein kinase kinase 2, MEK 3, SAPK kinase 2, SAPKK-2, SAPKK2, MEK6, MKK6, PRKMK6, SKK3, Dual specificity mitogen-activated protein kinase kinase 6, MAP kinase kinase 6, MAPKK 6, MAPK/ERK kinase 6, Stress-activated protein kinase kinase 3, MEK 6, SAPK kinase 3, SAPKK-3, SAPKK3

2 Images

Supplier Data

Western blot - Anti-MEK3 (phospho S189/T193) + MEK6 (phospho S207/T211) antibody (AB4759)

Two bands ~ 37 and 39 kDa corresponding to MEK3 (pS189/pT193) + MEK6 (pS207/pT211) was observed in Anisomycin treated cell line tested

All lanes:

Western blot - Anti-MEK3 (phospho S189/T193) + MEK6 (phospho S207/T211) antibody (ab4759) at 1/2000 dilution

Lane 1:

A549 whole cell extract at 20 µg

Lane 2:

A549 treated for 20 minutes with 200 nM of PMA at 20 µg

Lane 3:

A549 treated for 30 minutes with 25 µg/mL of Anisomycin at 20 µg

Lane 4:

A549 treated for 40 minutes with UV at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution

Predicted band size: 39.3 kDa

Observed band size: 37 kDa,39 kDa

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Unknown

Western blot - Anti-MEK3 (phospho S189/T193) + MEK6 (phospho S207/T211) antibody (AB4759)

Peptide Competition : Extracts prepared from background extracts with Mal-E tagged fusion protein expressing MEK 6 left inactivated (6) or with MEKK added for activation (1-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4^oC, then were incubated with 0.50 μg/mL ab4759 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with : no peptide (1, 6), the non-phosphopeptide corresponding to the phosphopeptide immunogen (2), a generic hosphoserine-containing peptide (3), a generic phosphothreonine-containing peptide (4), or the phosphopeptide immunogen (5). After washing, the membrane was incubated with goat F(ab"e;)2 anti-rabbit IgG alkaline phosphatase and signals were detected. The data show that only the phosphopeptide corresponding to MEK3/6 [pSpT189/193]/[pSpT207/211] blocks the antibody signal, demonstrating the specificity of the antibody. Please note that the Mal-E-tagged fusion protein expressing MEK6 runs at ~81 kDa.

All lanes:

Western blot - Anti-MEK3 (phospho S189/T193) + MEK6 (phospho S207/T211) antibody (ab4759)

Predicted band size: 39.3 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human MAP2K3 phospho S189 + T193. The exact immunogen used to generate this antibody is proprietary information.

P46734

Specificity

This antibody reacts with MEK 3 and MEK 6 (93% homologous). This antibody shows some cross-reactivity with MEK 4 when tested in a system with high MEK 4 expression levels. It does not react with any other MEK isoforms.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Synthetic peptide - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Product details

Mitogen Activated Protein Kinase Kinases 3 and 6 (MEK 3/6 or MKK 3/6) are 42 kDa members of a tyrosine/threonine protein kinase family that activate p38, which is part of the inflammation/stress signaling pathway. Phosphorylation of MEK3 and 6 by MEKK1 on serine 189 and threonine 193 (serine 207 and threonine 211 for MEK6) in the catalytic domain activates the proteins and enables them to phosphorylate p38.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated MEK 3. The final product is generated by affinity chromatography using a MEK 3 derived peptide that is phosphorylated at serine 189 and threonine 193.

Storage buffer

pH: 7.3 Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MEK3 also known as MAP2K3 and MEK6 also referred to as MAP2K6 are kinases in the mitogen-activated protein kinase (MAPK) signaling pathway. Mechanically MEK3 and MEK6 phosphorylate and activate p38 MAPKs which play important roles in cellular responses to stress and inflammation. MEK3 has a molecular mass of approximately 39 kDa and is widely expressed in various tissues including brain lung and liver. Similarly MEK6 is about 37 kDa in mass and shows expression in heart lung skeletal muscle and other tissues. Phosphorylation occurs at serine (S189 for MEK3 S207 for MEK6) and threonine (T193 for MEK3 T211 for MEK6) residues which are key to their activation.

Biological function summary

MEK3 and MEK6 function as essential components in the MAPK signaling cascade. These kinases work as upstream regulators that modulate the activity of p38 MAPK part of a larger MAPK complex. MEK3 and MEK6 transduce extracellular signals that trigger numerous cellular processes like differentiation apoptosis and response to stress stimuli. Their role influences a range of biological activities such as cell growth and survival indicating a vital role in maintaining cellular homeostasis.

Pathways

MEK3 and MEK6 are critical in the MAPK and the p38 MAPK signaling pathways. These pathways mediate responses to pro-inflammatory cytokines and environmental stress. Both kinases have connections to other proteins like MKK3 MKK6 and p38 MAPK translating extracellular cues into suitable cellular responses. Their function within these pathways impacts inflammatory responses and cell cycle regulation exemplifying their importance in cellular communication networks.

MEK3 and MEK6 relations include inflammatory conditions and cancer. Aberrant activation of these kinases leads to disease pathologies like rheumatoid arthritis and certain types of cancer. Moreover associations exist with other key proteins such as p38 MAPKs in these pathways which play important roles in disease progression. Understanding MEK3 and MEK6 roles allows for insights into developing targeted therapies for diseases linked to these kinases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14.

See full target information MAP2K3 phospho S189 + T193

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Biomaterials research 29:0201 PubMed40330275

2025

Impact of Culture Duration on the Properties and Functionality of Yeast-Derived Extracellular Vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Gyeongchan Jeon,Yang-Hoon Kim,Jiho Min

Human molecular genetics 33:1454-1464 PubMed38751339

2024

An endogenous retrovirus regulates tumor-specific expression of the immune transcriptional regulator SP140.

Applications

Unspecified application

Species

Unspecified reactive species

Adam K Dziulko,Holly Allen,Edward B Chuong

Nature communications 14:861 PubMed36792623

2023

Coadaptation fostered by the SLIT2-ROBO1 axis facilitates liver metastasis of pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Li,Xiao-Xin Zhang,Li-Peng Hu,Bo Ni,Dong-Xue Li,Xu Wang,Shu-Heng Jiang,Hui Li,Min-Wei Yang,Yong-Sheng Jiang,Chun-Jie Xu,Xue-Li Zhang,Yan-Li Zhang,Pei-Qi Huang,Qin Yang,Yang Zhou,Jian-Ren Gu,Gary Gui-Shan Xiao,Yong-Wei Sun,Jun Li,Zhi-Gang Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-MEK3 (phospho S189/T193) + MEK6 (phospho S207/T211) antibody

Western blot - Anti-MEK3 (phospho S189/T193) + MEK6 (phospho S207/T211) antibody (AB4759)

Western blot - Anti-MEK3 (phospho S189/T193) + MEK6 (phospho S207/T211) antibody (AB4759)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise