Rabbit Polyclonal MP2K6 antibody. Suitable for WB and reacts with Synthetic peptide - Human, Human samples. Immunogen corresponding to Synthetic Peptide within Human MAP2K3.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human MAP2K3. The exact immunogen used to generate this antibody is proprietary information. Database link P46734
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Synthetic peptide - Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Target data
Function
Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. With MAP3K3/MKK3, catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinases p38 MAPK11, MAPK12, MAPK13 and MAPK14 and plays an important role in the regulation of cellular responses to cytokines and all kinds of stresses. Especially, MAP2K3/MKK3 and MAP2K6/MKK6 are both essential for the activation of MAPK11 and MAPK13 induced by environmental stress, whereas MAP2K6/MKK6 is the major MAPK11 activator in response to TNF. MAP2K6/MKK6 also phosphorylates and activates PAK6. The p38 MAP kinase signal transduction pathway leads to direct activation of transcription factors. Nuclear targets of p38 MAP kinase include the transcription factors ATF2 and ELK1. Within the p38 MAPK signal transduction pathway, MAP3K6/MKK6 mediates phosphorylation of STAT4 through MAPK14 activation, and is therefore required for STAT4 activation and STAT4-regulated gene expression in response to IL-12 stimulation. The pathway is also crucial for IL-6-induced SOCS3 expression and down-regulation of IL-6-mediated gene induction; and for IFNG-dependent gene transcription. Has a role in osteoclast differentiation through NF-kappa-B transactivation by TNFSF11, and in endochondral ossification and since SOX9 is another likely downstream target of the p38 MAPK pathway. MAP2K6/MKK6 mediates apoptotic cell death in thymocytes. Acts also as a regulator for melanocytes dendricity, through the modulation of Rho family GTPases.
Additional Targets
MAP2K6
Alternative names
MEK6, MKK6, PRKMK6, SKK3, MAP2K6, Dual specificity mitogen-activated protein kinase kinase 6, MAP kinase kinase 6, MAPKK 6, MAPK/ERK kinase 6, Stress-activated protein kinase kinase 3, MEK 6, SAPK kinase 3, SAPKK-3, SAPKK3
Recommended products
Rabbit Polyclonal MP2K6 antibody. Suitable for WB and reacts with Synthetic peptide - Human, Human samples. Immunogen corresponding to Synthetic Peptide within Human MAP2K3.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human MAP2K3. The exact immunogen used to generate this antibody is proprietary information. Database link P46734
- Purification technique
- Affinity purification Immunogen
- Specificity
This antibody reacts with MEK 3 and MEK 6 (93% homologous). This antibody shows some cross-reactivity with MEK 4 when tested in a system with high MEK 4 expression levels. It does not react with any other MEK isoforms.
- Concentration
- Purification notes
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated MEK 3. The final product is generated by affinity chromatography using a MEK 3 derived peptide that is phosphorylated at serine 189 and threonine 193.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Mitogen Activated Protein Kinase Kinases 3 and 6 (MEK 3/6 or MKK 3/6) are 42 kDa members of a tyrosine/threonine protein kinase family that activate p38, which is part of the inflammation/stress signaling pathway. Phosphorylation of MEK3 and 6 by MEKK1 on serine 189 and threonine 193 (serine 207 and threonine 211 for MEK6) in the catalytic domain activates the proteins and enables them to phosphorylate p38.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MEK3 also known as MAP2K3 and MEK6 also referred to as MAP2K6 are kinases in the mitogen-activated protein kinase (MAPK) signaling pathway. Mechanically MEK3 and MEK6 phosphorylate and activate p38 MAPKs which play important roles in cellular responses to stress and inflammation. MEK3 has a molecular mass of approximately 39 kDa and is widely expressed in various tissues including brain lung and liver. Similarly MEK6 is about 37 kDa in mass and shows expression in heart lung skeletal muscle and other tissues. Phosphorylation occurs at serine (S189 for MEK3 S207 for MEK6) and threonine (T193 for MEK3 T211 for MEK6) residues which are key to their activation.
- Biological function summary
MEK3 and MEK6 function as essential components in the MAPK signaling cascade. These kinases work as upstream regulators that modulate the activity of p38 MAPK part of a larger MAPK complex. MEK3 and MEK6 transduce extracellular signals that trigger numerous cellular processes like differentiation apoptosis and response to stress stimuli. Their role influences a range of biological activities such as cell growth and survival indicating a vital role in maintaining cellular homeostasis.
- Pathways
MEK3 and MEK6 are critical in the MAPK and the p38 MAPK signaling pathways. These pathways mediate responses to pro-inflammatory cytokines and environmental stress. Both kinases have connections to other proteins like MKK3 MKK6 and p38 MAPK translating extracellular cues into suitable cellular responses. Their function within these pathways impacts inflammatory responses and cell cycle regulation exemplifying their importance in cellular communication networks.
- Associated diseases and disorders
MEK3 and MEK6 relations include inflammatory conditions and cancer. Aberrant activation of these kinases leads to disease pathologies like rheumatoid arthritis and certain types of cancer. Moreover associations exist with other key proteins such as p38 MAPKs in these pathways which play important roles in disease progression. Understanding MEK3 and MEK6 roles allows for insights into developing targeted therapies for diseases linked to these kinases.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-MEK3 (phospho S189/T193) + MEK6 (phospho S207/T211) antibody (ab4759)
Two bands ~ 37 and 39 kDa corresponding to MEK3 (pS189/pT193) + MEK6 (pS207/pT211) was observed in Anisomycin treated cell line tested
All lanes: Western blot - Anti-MEK3 (phospho S189/T193) + MEK6 (phospho S207/T211) antibody (ab4759) at 1/2000 dilution
Lane 1: A549 whole cell extract at 20 µg
Lane 2: A549 treated for 20 minutes with 200 nM of PMA at 20 µg
Lane 3: A549 treated for 30 minutes with 25 µg/mL of Anisomycin at 20 µg
Lane 4: A549 treated for 40 minutes with UV at 20 µg
SecondaryAll lanes: Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution
Predicted band size: 39.3 kDa
Observed band size: 37 kDa, 39 kDa
Western blot - Anti-MEK3 (phospho S189/T193) + MEK6 (phospho S207/T211) antibody (ab4759)
Peptide Competition: Extracts prepared from background extracts with Mal-E tagged fusion protein expressing MEK 6 left inactivated (6) or with MEKK added for activation (1-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50 μg/mL ab4759 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 6), the non-phosphopeptide corresponding to the phosphopeptide immunogen (2), a generic hosphoserine-containing peptide (3), a generic phosphothreonine-containing peptide (4), or the phosphopeptide immunogen (5). After washing, the membrane was incubated with goat F(ab"e;)2 anti-rabbit IgG alkaline phosphatase and signals were detected. The data show that only the phosphopeptide corresponding to MEK3/6 [pSpT189/193]/[pSpT207/211] blocks the antibody signal, demonstrating the specificity of the antibody. Please note that the Mal-E-tagged fusion protein expressing MEK6 runs at ~81 kDa.
All lanes: Western blot - Anti-MEK3 (phospho S189/T193) + MEK6 (phospho S207/T211) antibody (ab4759)
Predicted band size: 39.3 kDa
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