AB131494

Anti-MEK4/MKK4 antibody

Name: Anti-MEK4/MKK4 antibody (ab131494) | Abcam
Brand: Abcam Limited
SKU: AB131494
Price: 535 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal MEK4/MKK4 phospho S257 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human MAP2K4 phospho S257 aa 250-300 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

JNKK1, MEK4, MKK4, PRKMK4, SEK1, SERK1, SKK1, MAP2K4, Dual specificity mitogen-activated protein kinase kinase 4, MAP kinase kinase 4, MAPKK 4, JNK-activating kinase 1, MAPK/ERK kinase 4, SAPK/ERK kinase 1, Stress-activated protein kinase kinase 1, c-Jun N-terminal kinase kinase 1, MEK 4, SAPK kinase 1, SAPKK-1, SAPKK1, JNKK

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK4/MKK4 antibody (AB131494)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK4/MKK4 antibody (AB131494)

Immunohistochemical analysis of MEK4/MKK4 in paraffin embedded Human breast cancer tissue, using ab131494 at a 1/50 dilution. The image on the right was preincubated with blocking peptide.

Immunocytochemistry/ Immunofluorescence - Anti-MEK4/MKK4 antibody (AB131494)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MEK4/MKK4 antibody (AB131494)

Immunofluorescence analysis of MEK4/MKK4 in methanol fixed HeLa cells stained with ab131494 at a 1/100 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human MAP2K4 phospho S257 aa 250-300 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P45985

Specificity

ab131494 detects endogenous levels of total MEK4 protein.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "<p>(methanol fixed cells)</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab131494 was purified by affinity chromatography using an epitope specific phosphopeptide. Non phosphopeptide specific antibodies were removed by chromatography using non phosphopeptide.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MEK4 also known as MKK4 is a protein kinase with a molecular weight of approximately 43 kDa. It functions as a dual-specificity kinase that phosphorylates and activates other kinases in cellular signaling pathways. MEK4 appears in various tissues including heart brain and skeletal muscle. It acts as an essential component in transmitting extracellular signals to downstream effectors and is involved in critical cellular processes like proliferation differentiation and apoptosis.

Biological function summary

MEK4 acts as an upstream kinase in the MAPK signaling cascade. Through its role in this pathway it is part of a larger signaling complex. MEK4 specifically activates JUN N-terminal kinase (JNK) by phosphorylation. JNK further regulates gene expression by influencing transcription factors impacting cellular responses. This makes MEK4 a pivotal element in facilitating messenger roles that allow cells to adapt to changing environments such as stress responses and immune reactions.

Pathways

MEK4 plays significant roles in the MAPK and stress-activated protein kinase (SAPK) pathways. In the MAPK pathway MEK4 phosphorylates JNK thereby influencing cellular responses to environmental changes. Within the SAPK pathway MEK4 interacts with other kinases such as MKK7 to modulate cellular responses further. The flow of signals through these pathways affects cell fate cell cycle regulation and programmed cell death demonstrating its comprehensive influence on cell physiology.

MEK4 shows involvement in cancer progression and neurodegenerative diseases. Abnormal activation of MEK4 can contribute to tumor growth and metastasis in cancers such as prostate and ovarian cancer by enhancing JNK signaling. In neurodegenerative disorders deregulation of MEK4-related pathways might lead to neuronal damage or apoptosis. Proteins like JNK and other MAP kinases become dysregulated in these conditions illustrating the interconnected role of MEK4 in disease mechanisms. Phospho flow cytometry can be utilized to measure alterations in MEK4 phosphorylation states aiding in elucidating its role in disease contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 seems to be the prerequisite for JNK activation at least in response to pro-inflammatory cytokines, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs. MAP2K4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Whereas MAP2K7/MKK7 exclusively activates JNKs, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAPK12, MAPK13 and MAPK14.

See full target information MAP2K4 phospho S257

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:109048 PubMed38464592

2024

Specific knockout of macrophage SHP2 promotes macrophage M2 polarization and alleviates renal ischemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Meilian Du,Shanbao Zhang,Xiaoyu Wang,Chen Liu,Linrong Pan,Xiao Chen,Yinghui Qi

BMC complementary medicine and therapies 20:261 PubMed32843018

2020

Anti-inflammation effects of the total saponin fraction from Dioscorea nipponica Makino on rats with gouty arthritis by influencing MAPK signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Zhou,Hui Juan Sun,Shu Min Liu,Xi Hong Jiang,Qiu Yue Wang,Shuang Zhang,Dong Hua Yu

Frontiers in aging neuroscience 12:23 PubMed32116652

2020

Electroacupuncture Ameliorates Cognitive Impairment by Inhibiting the JNK Signaling Pathway in a Mouse Model of Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yinshan Tang,Anping Xu,Shujun Shao,You Zhou,Bing Xiong,Zhigang Li

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