AB210748

Anti-MEK5 antibody

Name: Anti-MEK5 antibody (ab210748) | Abcam
Brand: Abcam Limited
SKU: AB210748
Price: 510 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal MEK5 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MAP2K5 aa 1-200.

View Alternative Names

MEK5, MKK5, PRKMK5, MAP2K5, Dual specificity mitogen-activated protein kinase kinase 5, MAP kinase kinase 5, MAPKK 5, MAPK/ERK kinase 5, MEK 5

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-MEK5 antibody (AB210748)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MEK5 antibody (AB210748)

Immunofluorescence analysis of U20S cells labeling MEK5 with ab210748 (red) at a 1/50 dilution. Nuclei were counterstained with DAPI (blue).

Supplier Data

Western blot - Anti-MEK5 antibody (AB210748)

All lanes:

Western blot - Anti-MEK5 antibody (ab210748) at 1/500 dilution

Lane 1:

Extracts from H460 cell line

Lane 2:

Extracts from BT474 cell line

Lane 3:

Extracts from mouse liver

Lane 4:

Extracts from rat liver

Lane 5:

Extracts from rat kidney

Predicted band size: 50 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human MAP2K5 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q13163

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MEK5 also known as MAP2K5 is a dual specificity protein kinase encoded by the MAP2K5 gene. The protein has a molecular weight of approximately 49 kDa. MEK5 is expressed in various tissues with higher expression levels in the heart skeletal muscle and brain. It functions mechanically by activating and phosphorylating ERK5 a member of the MAPK family thereby playing a role in transmitting signals from the cell surface to the nucleus.

Biological function summary

MEK5 plays a critical role in cell growth and survival. MEK5 does not work alone; it associates with MEK5/ERK5 signaling complex. This complex is significant in mediating cellular responses to growth factors and stress stimuli. MEK5’s activation of ERK5 facilitates regulation of various cellular activities including proliferation and differentiation.

Pathways

MEK5 functions in the ERK5 signaling pathway and the MAP kinase cascade. These pathways are important for transducing extracellular signals leading to specific cellular responses. MEK5 interacts closely with ERK5 (also known as MAPK7) in these pathways helping in the transduction of mitogen signals essential for many cellular processes.

MEK5 has implications in cancer and cardiovascular diseases. Abnormal MEK5 signaling is associated with certain cancer types including prostate cancer where it influences cell proliferation and survival. The MEK5/ERK5 pathway is also linked to cardiovascular diseases such as hypertrophy where it plays a role in the stress response of cardiac cells. In these contexts overactive MEK5 signaling contributes to disease progression or pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appears to play a critical role in protecting cells from stress-induced apoptosis, neuronal survival and cardiac development and angiogenesis. As part of the MAPK/ERK signaling pathway, acts as a negative regulator of apoptosis in cardiomyocytes via promotion of STUB1/CHIP-mediated ubiquitination and degradation of ICER-type isoforms of CREM (By similarity).

See full target information MAP2K5

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

European journal of medical research 30:117 PubMed39972514

2025

MEK5-ERK5 pathway mediates mitophagy by regulating Nur77 to promote tumorigenesis of osteosarcoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jianshu Wang,Jinxu Xue,Baijing Ma,Yanqi Zhu,Jing Li,Caiping Tian

The Journal of clinical investigation 134: PubMed38875287

2024

Liver cancer initiation requires translational activation by an oncofetal regulon involving LIN28 proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Meng-Hsiung Hsieh,Yonglong Wei,Lin Li,Liem H Nguyen,Yu-Hsuan Lin,Jung M Yong,Xuxu Sun,Xun Wang,Xin Luo,Sarah K Knutson,Christina Bracken,George Q Daley,John T Powers,Hao Zhu

Aging 13:16088-16104 PubMed34176788

2021

High glucose exacerbates neuroinflammation and apoptosis at the intermediate stage after post-traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Wenqian Zhang,Jun Hong,Wencheng Zheng,Aijun Liu,Ying Yang

International journal of endocrinology 2020:8821077 PubMed33488706

2020

Liraglutide Promotes Osteoblastic Differentiation in MC3T3-E1 Cells by ERK5 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Sun,Yuzhen Liang,Zhengming Li,Ning Xia

International journal of cancer 144:1321-1330 PubMed30132833

2018

Whole exome and target sequencing identifies MAP2K5 as novel susceptibility gene for familial non-medullary thyroid carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Ye,Hongwei Gao,Lin Xiao,Zhixiang Zuo,Yueping Liu,Qi Zhao,Huijiao Chen,Weiyi Feng,Bo Fu,Linyong Sun,Xiaolin Jiang,Du He,He Jiang,Mei Yang,Li Li,Fei Chen,Xiang Liu,Shuang Li,Zhihui Li,Yong Jiang,Liang Cheng,Hong Bu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MEK5 antibody

Immunocytochemistry/ Immunofluorescence - Anti-MEK5 antibody (AB210748)

Western blot - Anti-MEK5 antibody (AB210748)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise