AB154362

Anti-MEKK3 antibody

Name: Anti-MEKK3 antibody (ab154362) | Abcam
Brand: Abcam Limited
SKU: AB154362
Price: 545 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal MEKK3 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MAP3K3 aa 250 to C-terminus.

View Alternative Names

MAPKKK3, MEKK3, MAP3K3, Mitogen-activated protein kinase kinase kinase 3, MAPK/ERK kinase kinase 3, MEK kinase 3, MEKK 3

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEKK3 antibody (AB154362)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEKK3 antibody (AB154362)

Immunohistochemical analysis of paraffin-embedded Human Cal27 xenograft tissue, labeling MEKK3 with ab154362 at 1/500 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEKK3 antibody (AB154362)

Immunohistochemical analysis of paraffin-embedded Human S-G xenograft tissue, labeling MEKK3 with ab154362 at 1/500 dilution.

Supplier Data

Western blot - Anti-MEKK3 antibody (AB154362)

Samples were separated by 0.075 SDS-PAGE.

All lanes:

Western blot - Anti-MEKK3 antibody (ab154362) at 1/20000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

3xFlag-human MAP3K3-transfected 293T whole cell extracts at 30 µg

Predicted band size: 71 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEKK3 antibody (AB154362)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue labelling MEKK3 protein at cytoplasm and nucleus with ab154362 at a 1/500 dilution.
Antigen retrieval : Citrate buffer, pH 6.0, 15 min

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEKK3 antibody (AB154362)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labelling MEKK3 protein at cytoplasm and nucleus with ab154362 at a 1/500 dilution.
Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MAP3K3 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q99759

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Alternatively Tris-EDTA buffer (pH8.0) may be used. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MEKK3 also known as MAP3K3 is a serine/threonine kinase that plays an important role in the MAPK signaling cascade. This protein has a molecular mass of approximately 67 kDa. MEKK3 is widely expressed with notable presence in tissues like the heart placenta and skeletal muscles. It is important for transmitting signals from the cell surface to various intracellular targets impacting numerous cellular processes.

Biological function summary

MEKK3 participates in cell growth differentiation and apoptosis regulation. It often functions within a multi-protein complex including components like MEK5 and ERK5. MEKK3 activates downstream kinases influencing gene expression and cellular responses to external signals. Its involvement is essential for controlling reactions to stress and inflammatory stimuli.

Pathways

MEKK3 integrates into the MAPK and NF-κB pathways both critical for mediating signaling responses. In the MAPK pathway MEKK3 regulates kinases like MEK5 and ERK5. Additionally it connects with proteins such as IKK in the NF-κB pathway influencing immunity and cell survival. These interactions position MEKK3 centrally in cellular defense and adaptability mechanisms.

MEKK3 associates with pathological conditions like cardiovascular diseases and cancer. Its role in these disorders links closely with dysregulation in the MAPK pathway where MEK5 and ERK5 also play significant roles. Furthermore aberrations in the NF-κB pathway involving IKK can contribute to its involvement in these diseases highlighting MEKK3's impact on disease progression and potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators.

See full target information MAP3K3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Communications medicine 5:110 PubMed40216942

2025

Rescue of lysosomal acid lipase deficiency in mice by rAAV8 liver gene transfer.

Applications

Unspecified application

Species

Unspecified reactive species

Marine Laurent,Rim Harb,Christine Jenny,Julie Oustelandt,Simon Jimenez,Jeremie Cosette,Francesca Landini,Aristide Ferrante,Guillaume Corre,Nemanja Vujic,Claudia Piccoli,Anais Brassier,Laetitia Van Wittenberghe,Giuseppe Ronzitti,Dagmar Kratky,Consiglia Pacelli,Mario Amendola

International journal of molecular medicine 43:1203-1216 PubMed30628663

2019

miR‑505 inhibits cell growth and EMT by targeting MAP3K3 through the AKT‑NFκB pathway in NSCLC cells.

Applications

Unspecified application

Species

Unspecified reactive species

Huaping Tang,Weihong Lv,Wenxin Sun,Qiaojie Bi,Yueqin Hao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MEKK3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEKK3 antibody (AB154362)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEKK3 antibody (AB154362)

Western blot - Anti-MEKK3 antibody (AB154362)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEKK3 antibody (AB154362)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEKK3 antibody (AB154362)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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