Anti-MEKK3 antibody [ep600y] - BSA and Azide free

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-MEKK3 antibody [ep600y] - BSA and Azide free (AB239826)

This data was developed using ab40756, the same antibody clone in a different buffer formulation.

Flow Cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labeling MEKK3 with purified ab40756 at 1/100 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEKK3 antibody [ep600y] - BSA and Azide free (AB239826)

Immunohistochemical analysis of paraffin-embedded human ovary carcinoma using ab40756 diluted 1 : 100

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40756).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-MEKK3 antibody [ep600y] - BSA and Azide free (AB239826)

This data was developed using ab40756, the same antibody clone in a different buffer formulation.

Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling MEKK3 with purified ab40756 at 1/1000 dilution (1 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MEKK3 antibody [ep600y] - BSA and Azide free (AB239826)

Immunofluorescent staining of HeLa cells using ab40756 diluted 1 : 100This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40756).

• IP

Lab

Immunoprecipitation - Anti-MEKK3 antibody [ep600y] - BSA and Azide free (AB239826)

71 kDa MEKK3 was immunoprecipitated from 0.35mg of bEnd.3 (mouse brain endothelial cell) whole cell lysate with ab40756 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab40756 at 1/200 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution. Lane 1 (Input) : bEnd.3 (mouse brain endothelial cell) whole cell lysate 10 μg. Lane 2 (+) : bEnd.3 whole cell lysate. Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab40756 in bEnd.3 whole cell lysate Blocking buffer and concentration : 5% NFDM/TBST. Diluting buffer and concentration : 5% NFDM/TBST. Exposure time : 180 seconds. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40756).

All lanes:

Immunoprecipitation - Anti-MEKK3 antibody [ep600y] (<a href='/en-us/products/primary-antibodies/mekk3-antibody-ep600y-ab40756'>ab40756</a>) at 1/200 dilution

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Predicted band size: 71 kDa

Observed band size: 71 kDa

false

Exposure time: 180s