AB5267

Anti-Mel18 antibody

Name: Anti-Mel18 antibody (ab5267) | Abcam
Brand: Abcam Limited
SKU: AB5267
Price: 565 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(16 Publications)

Goat Polyclonal Mel18 antibody. Suitable for WB and reacts with Human samples. Cited in 16 publications. Immunogen corresponding to Synthetic Peptide within Human PCGF2 aa 300 to C-terminus.

View Alternative Names

MEL18, RNF110, ZNF144, PCGF2, Polycomb group RING finger protein 2, DNA-binding protein Mel-18, RING finger protein 110, Zinc finger protein 144

1 Images

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Western blot - Anti-Mel18 antibody (AB5267)

ab5267 staining (1μg/ml) of Human Lung lysate (RIPA buffer, 30μg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

ab5267 staining (1µg/ml) of Human Lung lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

All lanes:

Western blot - Anti-Mel18 antibody (ab5267)

Predicted band size: 38 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human PCGF2 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P35227

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional repressor. Binds specifically to the DNA sequence 5'-GACTNGACT-3'. Has tumor suppressor activity. May play a role in control of cell proliferation and/or neural cell development. Regulates proliferation of early T progenitor cells by maintaining expression of HES1. Also plays a role in antero-posterior specification of the axial skeleton and negative regulation of the self-renewal activity of hematopoietic stem cells (By similarity). Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed : 26151332). Within the PRC1-like complex, regulates RNF2 ubiquitin ligase activity (PubMed : 26151332).

See full target information PCGF2

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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 14:1371-1389 PubMed38389850

2024

Polycomb repressive complex 1 modulates granulosa cell proliferation in early folliculogenesis to support female reproduction.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Gao,Tuo Zhang,Tengxiang Chen,Ziqi Chen,Zijian Zhu,Yang Wen,Shaogang Qin,Yibing Bao,Ting Zhao,Hengxing Li,Longping Liu,Ming Hao,Jianbin Wang,Fengchao Wang,Haibin Wang,Bo Zhou,Hua Zhang,Guoliang Xia,Chao Wang

The Journal of clinical investigation 131: PubMed34128475

2021

BMI1 maintains the Treg epigenomic landscape to prevent inflammatory bowel disease.

Applications

Unspecified application

Species

Unspecified reactive species

Michelle M Gonzalez,Adebowale O Bamidele,Phyllis A Svingen,Mary R Sagstetter,Thomas C Smyrk,Joseph M Gaballa,Feda H Hamdan,Robyn Laura Kosinsky,Hunter R Gibbons,Zhifu Sun,Zhenqing Ye,Asha Nair,Guilherme P Ramos,Manuel B Braga Neto,Alexander Q Wixom,Angela J Mathison,Steven A Johnsen,Raul Urrutia,William A Faubion

Oncology letters 17:5012-5022 PubMed31186712

2019

Co-inhibition of BMI1 and Mel18 enhances chemosensitivity of esophageal squamous cell carcinoma and .

Applications

Unspecified application

Species

Unspecified reactive species

Jiansong Wang,Huaijun Ji,Qiang Zhu,Xinshuang Yu,Juan Du,Zhongmin Jiang

Nucleic acids research 47:2807-2821 PubMed30649516

2019

Requirement for PRC1 subunit BMI1 in host gene activation by Epstein-Barr virus protein EBNA3C.

Applications

Unspecified application

Species

Unspecified reactive species

Kostas Paschos,Quentin Bazot,Jonathan Lees,Paul J Farrell,Martin J Allday

PLoS genetics 9:e1003588 PubMed23861667

2013

Alu elements in ANRIL non-coding RNA at chromosome 9p21 modulate atherogenic cell functions through trans-regulation of gene networks.

Applications

Unspecified application

Species

Unspecified reactive species

Lesca M Holdt,Steve Hoffmann,Kristina Sass,David Langenberger,Markus Scholz,Knut Krohn,Knut Finstermeier,Anika Stahringer,Wolfgang Wilfert,Frank Beutner,Stephan Gielen,Gerhard Schuler,Gabor Gäbel,Hendrik Bergert,Ingo Bechmann,Peter F Stadler,Joachim Thiery,Daniel Teupser

Nature cell biology 15:353-62 PubMed23502315

2013

Polycomb Cbx family members mediate the balance between haematopoietic stem cell self-renewal and differentiation.

Applications

Species

Unspecified reactive species

Karin Klauke,Višnja Radulović,Mathilde Broekhuis,Ellen Weersing,Erik Zwart,Sandra Olthof,Martha Ritsema,Sophia Bruggeman,Xudong Wu,Kristian Helin,Leonid Bystrykh,Gerald de Haan

Genes & development 26:1445-58 PubMed22713603

2012

Bmi1 facilitates primitive endoderm formation by stabilizing Gata6 during early mouse development.

Applications

Unspecified application

Species

Mouse

Fabrice Lavial,Sylvain Bessonnard,Yusuke Ohnishi,Akiko Tsumura,Anil Chandrashekran,Mark A Fenwick,Rute A Tomaz,Hiroyuki Hosokawa,Toshinori Nakayama,Ian Chambers,Takashi Hiiragi,Claire Chazaud,Véronique Azuara

Cell 148:664-78 PubMed22325148

2012

RYBP-PRC1 complexes mediate H2A ubiquitylation at polycomb target sites independently of PRC2 and H3K27me3.

Applications

Species

Mouse

Lígia Tavares,Emilia Dimitrova,David Oxley,Judith Webster,Raymond Poot,Jeroen Demmers,Karel Bezstarosti,Stephen Taylor,Hiroki Ura,Hiroshi Koide,Anton Wutz,Miguel Vidal,Sarah Elderkin,Neil Brockdorff

Biochemical and biophysical research communications 416:86-91 PubMed22085718

2011

Inhibition of PCGF2 enhances granulocytic differentiation of acute promyelocytic leukemia cell line HL-60 via induction of HOXA7.

Applications

Unspecified application

Species

Unspecified reactive species

Sungsin Jo,Hongki Lee,Sojin Kim,Eun Mi Hwang,Jae-Yong Park,Sang Soo Kang,Heekyoung Chung

Nature structural & molecular biology 17:862-8 PubMed20543829

2010

Role for the MOV10 RNA helicase in polycomb-mediated repression of the INK4a tumor suppressor.

Applications

Species

Human

Selma El Messaoudi-Aubert,James Nicholls,Goedele N Maertens,Sharon Brookes,Emily Bernstein,Gordon Peters

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Mel18 antibody

Western blot - Anti-Mel18 antibody (AB5267)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (16)

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Primary Antibodies

Primary Antibodies

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