Mouse Monoclonal MelanA antibody. Suitable for IHC-P, IP, Flow Cyt, WB, ICC/IF, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 7 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MLANA.
Images
![Western blot - Anti-MelanA antibody [M2-9E3] (AB187369), expandable thumbnail](https://content.abcam.com/products/images/melana-antibody-m2-9e3-ab187369--western-blot-img45326.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MelanA antibody [M2-9E3] (AB187369)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [M2-9E3] (AB187369), expandable thumbnail](https://content.abcam.com/products/images/melana-antibody-m2-9e3-ab187369--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img19240.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [M2-9E3] (AB187369)")
Publications
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- Oncology letters 26:4092023Renal epithelioid angiomyolipoma: A case report.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongyun Li,Xuebei Zhang,Qingli Zhao,Jin Wang,Xiaoqing Yang,Shengliang HuangPubMed 37600337
- Cell death discovery 8:702022Oxeiptosis: a novel pathway of melanocytes death in response to oxidative stress in vitiligo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPan Kang,Jianru Chen,Weigang Zhang,Ningning Guo,Xiuli Yi,Tingting Cui,Jiaxi Chen,Yuqi Yang,Yinghan Wang,Pengran Du,Zhubiao Ye,Baizhang Li,Chunying Li,Shuli LiPubMed 35177586
- Journal of toxicologic pathology 34:151-1562021Orbital malignant schwannoma and embryonal rhabdomyosarcoma in rats caused by leakage of locally injected nickel subsulfide to the orbit.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoshinori Yamagiwa,Miki Masatsugu,Haruna Tahara,Kotaro Yamada,Yu Haranosono,Masaaki Kurata,Hiroshi SatohPubMed 33976471
- Free radical biology & medicine 139:80-912019Oxidative stress-induced IL-15 trans-presentation in keratinocytes contributes to CD8 T cells activation via JAK-STAT pathway in vitiligo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuguang Chen et. al.PubMed 31078730
- Journal of cellular and molecular medicine 22:2944-29542018Up-regulated deubiquitinase USP4 plays an oncogenic role in melanoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeinan Guo et. al.PubMed 29542252
- American journal of human genetics 102:219-2322018Loss of GPNMB Causes Autosomal-Recessive Amyloidosis Cutis Dyschromica in Humans.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChi-Fan Yang et. al.PubMed 29336782
- Oncology letters 11:95-982016Malignant renal epithelioid angiomyolipoma: A case report and review of the literature.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBaoyin Guo,Hualin Song,Jiuling Yue,Gang LiPubMed 26870174
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human MLANA. Database link Q16655
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | ICC/IF | IHC-Fr | |
|---|---|---|---|---|---|---|
| Human | Tested | Expected | Expected | Tested | Expected | Expected |
| Mouse | Predicted | Expected | Expected | Predicted | Expected | Expected |
| Rat | Predicted | Expected | Expected | Predicted | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5-1 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Species Rat | Dilution info - | Notes ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Species Human | Dilution info - | Notes ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-Fr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
6 products for Alternative Product
Target data
Function
Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.
Alternative names
MART1, MLANA, Melanoma antigen recognized by T-cells 1, MART-1, Antigen LB39-AA, Antigen SK29-AA, Protein Melan-A
Recommended products
Mouse Monoclonal MelanA antibody. Suitable for IHC-P, IP, Flow Cyt, WB, ICC/IF, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 7 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MLANA.
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human MLANA. Database link Q16655
- Clone number
- M2-9E3
- Purification technique
- Affinity purification Protein A
- Light chain type
- kappa
- Concentration
- Purification notes
ab187369 was purified from Bioreactor Concentrate by Protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-MelanA antibody [M2-9E3] (ab187369), expandable thumbnail](https://content.abcam.com/products/images/melana-antibody-m2-9e3-ab187369--western-blot-img45326.jpg "Western blot - Anti-MelanA antibody [M2-9E3] (ab187369)")
Western blot - Anti-MelanA antibody [M2-9E3] (ab187369)
Western blot analysis of COLO38 cell lysate labeling MelanA with ab187369 at 1 ug/ml.
All lanes: Western blot - Anti-MelanA antibody [M2-9E3] (ab187369) at 1 µg/mL
All lanes: Human COLO38 cell lysate
Predicted band size: 13 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [M2-9E3] (ab187369), expandable thumbnail](https://content.abcam.com/products/images/melana-antibody-m2-9e3-ab187369--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img19240.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [M2-9E3] (ab187369)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [M2-9E3] (ab187369)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human melanoma tissue labeling MelanA with ab187369 at 1 μg/ml.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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