Mouse Monoclonal MelanA antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Dog, African green monkey samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MLANA.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [OTI3E2] (AB140503), expandable thumbnail](https://content.abcam.com/products/images/melana-antibody-oti3e2-ab140503--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115681.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [OTI3E2] (AB140503)")
![Immunocytochemistry/ Immunofluorescence - Anti-MelanA antibody [OTI3E2] (AB140503), expandable thumbnail](https://content.abcam.com/products/images/melana-antibody-oti3e2-ab140503--immunocytochemistry-immunofluorescence-img1756.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-MelanA antibody [OTI3E2] (AB140503)")
![Western blot - Anti-MelanA antibody [OTI3E2] (AB140503), expandable thumbnail](https://content.abcam.com/products/images/melana-antibody-oti3e2-ab140503--western-blot-img146246.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MelanA antibody [OTI3E2] (AB140503)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [OTI3E2] (AB140503), expandable thumbnail](https://content.abcam.com/products/images/melana-antibody-oti3e2-ab140503--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133989.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [OTI3E2] (AB140503)")
![Western blot - Anti-MelanA antibody [OTI3E2] (AB140503), expandable thumbnail](https://content.abcam.com/products/images/melana-antibody-oti3e2-ab140503--western-blot-img160492.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MelanA antibody [OTI3E2] (AB140503)")
Publications
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- The British journal of dermatology 185:391-4042021Opsin 5 is a key regulator of ultraviolet radiation-induced melanogenesis in human epidermal melanocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesY Lan,W Zeng,X Dong,H LuPubMed 33400324
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human MLANA. Database link Q16655
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Expected |
| African green monkey | Expected | Expected | Tested |
| Dog | Tested | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/4000 | Notes - |
Species Dog | Dilution info 1/4000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species African green monkey | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/150 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Dog, African green monkey | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species African green monkey | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Dog | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.
Alternative names
MART1, MLANA, Melanoma antigen recognized by T-cells 1, MART-1, Antigen LB39-AA, Antigen SK29-AA, Protein Melan-A
Recommended products
Mouse Monoclonal MelanA antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Dog, African green monkey samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MLANA.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human MLANA. Database link Q16655
- Clone number
- OTI3E2
- Purification technique
- Affinity purification
- Concentration
- Purification notes
Purified from cell culture supernatant by affinity chromatography
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
The clone number has been updated from 3E2 to OTI3E2, both clone numbers name the same clone.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [OTI3E2] (ab140503), expandable thumbnail](https://content.abcam.com/products/images/melana-antibody-oti3e2-ab140503--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115681.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [OTI3E2] (ab140503)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [OTI3E2] (ab140503)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling MelanA with ab140503 at 1/150 dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-MelanA antibody [OTI3E2] (ab140503), expandable thumbnail](https://content.abcam.com/products/images/melana-antibody-oti3e2-ab140503--immunocytochemistry-immunofluorescence-img1756.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MelanA antibody [OTI3E2] (ab140503)")
Immunocytochemistry/ Immunofluorescence - Anti-MelanA antibody [OTI3E2] (ab140503)
Immunofluorescent analysis of COS7 cells transiently transfected with pCMV6-ENTRY MelanA, labelling MelanA with ab140503 at 1/100 dilution.
![Western blot - Anti-MelanA antibody [OTI3E2] (ab140503), expandable thumbnail](https://content.abcam.com/products/images/melana-antibody-oti3e2-ab140503--western-blot-img146246.jpg "Western blot - Anti-MelanA antibody [OTI3E2] (ab140503)")
Western blot - Anti-MelanA antibody [OTI3E2] (ab140503)
All lanes: Western blot - Anti-MelanA antibody [OTI3E2] (ab140503) at 1/200 dilution
All lanes: MDCK cell lysate at 10 µg
Predicted band size: 13 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [OTI3E2] (ab140503), expandable thumbnail](https://content.abcam.com/products/images/melana-antibody-oti3e2-ab140503--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133989.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [OTI3E2] (ab140503)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [OTI3E2] (ab140503)
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labelling MelanA with ab140503 at 1/150 dilution.
![Western blot - Anti-MelanA antibody [OTI3E2] (ab140503), expandable thumbnail](https://content.abcam.com/products/images/melana-antibody-oti3e2-ab140503--western-blot-img160492.jpg "Western blot - Anti-MelanA antibody [OTI3E2] (ab140503)")
Western blot - Anti-MelanA antibody [OTI3E2] (ab140503)
All lanes: Western blot - Anti-MelanA antibody [OTI3E2] (ab140503) at 1/4000 dilution
Lane 1: HEK293T cell lysate transfected with pCMV6-ENTRY control at 5 µg
Lane 2: HEK293T cell lysate transfected with pCMV6-ENTRY MelanA at 5 µg
Predicted band size: 13 kDa
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