Mouse Multiclonal Melanoma antibody. Suitable for Flow Cyt, IHC-P, IHC-Fr and reacts with Human samples. Cited in 29 publications.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [HMB45 + M2-7C10 + M2-9E3] (AB732), expandable thumbnail](https://content.abcam.com/products/images/melanoma-antibody-hmb45-m2-7c10-m2-9e3-ab732--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img23054.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [HMB45 + M2-7C10 + M2-9E3] (AB732)")
Publications
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- International journal of molecular sciences 24:2023The β Isoform of Human ATP-Binding Cassette B5 Transporter, ABCB5β, Localizes to the Endoplasmic Reticulum.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdriana María Díaz-Anaya,Louise Gerard,Martine Albert,Jean-François Gaussin,Marielle Boonen,Jean-Pierre GilletPubMed 37958830
- Proceedings of the National Academy of Sciences of the United States of America 120:e22143501202023Circulating galectin-1 delineates response to bevacizumab in melanoma patients and reprograms endothelial cell biology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNadia Bannoud,Juan C Stupirski,Alejandro J Cagnoni,Pablo F Hockl,Juan M Pérez Sáez,P Alfredo García,Yamil D Mahmoud,Julián Gambarte Tudela,Marco A Scheidegger,Andrea Marshall,Pippa G Corrie,Mark R Middleton,Karina V Mariño,M Romina Girotti,Diego O Croci,Gabriel A RabinovichPubMed 36634146
- Nature medicine 28:752-7652022Stratification of radiosensitive brain metastases based on an actionable S100A9/RAGE resistance mechanism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCátia Monteiro,Lauritz Miarka,María Perea-García,Neibla Priego,Pedro García-Gómez,Laura Álvaro-Espinosa,Ana de Pablos-Aragoneses,Natalia Yebra,Diana Retana,Patricia Baena,Coral Fustero-Torre,Osvaldo Graña-Castro,Kevin Troulé,Eduardo Caleiras,Patricia Tezanos,Pablo Muela,Elisa Cintado,José Luis Trejo,Juan Manuel Sepúlveda,Pedro González-León,Luis Jiménez-Roldán,Luis Miguel Moreno,Olga Esteban,Ángel Pérez-Núñez,Aurelio Hernández-Lain,José Mazarico Gallego,Irene Ferrer,Rocío Suárez,Eva M Garrido-Martín,Luis Paz-Ares,Celine Dalmasso,Elizabeth Cohen-Jonathan Moyal,Aurore Siegfried,Aisling Hegarty,Stephen Keelan,Damir Varešlija,Leonie S Young,Malte Mohme,Yvonne Goy,Harriet Wikman,Jose Fernández-Alén,Guillermo Blasco,Lucía Alcázar,Clara Cabañuz,Sergei I Grivennikov,Andrada Ianus,Noam Shemesh,Claudia C Faria,Rebecca Lee,Paul Lorigan,Emilie Le Rhun,Michael Weller,Riccardo Soffietti,Luca Bertero,Umberto Ricardi,Joaquim Bosch-Barrera,Elia Sais,Eduard Teixidor,Alejandro Hernández-Martínez,Alfonso Calvo,Javier Aristu,Santiago M Martin,Alvaro Gonzalez,Omer Adler,Neta Erez,Manuel ValientePubMed 35411077
- Cancers 13:2021Sensitivity and Resistance of Oncogenic RAS-Driven Tumors to Dual MEK and ERK Inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAntonella Catalano,Mojca Adlesic,Thorsten Kaltenbacher,Rhena F U Klar,Joachim Albers,Philipp Seidel,Laura P Brandt,Tomas Hejhal,Philipp Busenhart,Niklas Röhner,Kyra Zodel,Kornelia Fritsch,Peter J Wild,Justus Duyster,Ralph Fritsch,Tilman Brummer,Ian J FrewPubMed 33924486
- Oncogene 40:1792-18052021Classification of node-positive melanomas into prognostic subgroups using keratin, immune, and melanogenesis expression patterns.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDvir Netanely,Stav Leibou,Roma Parikh,Neta Stern,Hananya Vaknine,Ronen Brenner,Sarah Amar,Rivi Haiat Factor,Tomer Perluk,Jacob Frand,Eran Nizri,Dov Hershkovitz,Valentina Zemser-Werner,Carmit Levy,Ron ShamirPubMed 33564068
- ESMO open 5:2020Brain microenvironment-driven resistance to immune and targeted therapies in acral melanoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRebecca Jane Lee,Garima Khandelwal,Franziska Baenke,Alessio Cannistraci,Kenneth Macleod,Piyushkumar Mundra,Garry Ashton,Amit Mandal,Amaya Viros,Gabriela Gremel,Elena Galvani,Matthew Smith,Neil Carragher,Nathalie Dhomen,Crispin Miller,Paul Lorigan,Richard MaraisPubMed 32817058
- Cell reports 29:3200-3211.e42019YAP/TAZ Activation Drives Uveal Melanoma Initiation and Progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuapeng Li,Qi Li,Kyvan Dang,Shan Ma,Jennifer L Cotton,Sun Yang,Lihua J Zhu,April C Deng,Y Tony Ip,Randy L Johnson,Xu Wu,Claudio Punzo,Junhao MaoPubMed 31801083
- Science signaling 12:2019Adipocytes sensitize melanoma cells to environmental TGF-β cues by repressing the expression of miR-211.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTamar Golan et. al.PubMed 31337739
- Molecular cell 72:444-456.e72018UV-Protection Timer Controls Linkage between Stress and Pigmentation Skin Protection Systems.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHagar Malcov-Brog et. al.PubMed 30401431
- Integrative cancer therapies 17:646-6532018In Vivo Antitumor Effects of MK615 Led by PD-L1 Downregulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMasashi Yanaki et. al.PubMed 29665734
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 97.5% Water, 0.99% BSA, 0.33% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.007% Hydrochloric acid- Form
- Liquid
- Clonality
- Multiclonal
Consider this alternative
Reactivity data
Select an application| Flow Cyt | IHC-P | IHC-Fr | |
|---|---|---|---|
| Human | Expected | Tested | Expected |
| Rat | Predicted | Predicted | Predicted |
Flow Cyt
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/200 | Notes The staining intensity of this melanoma cocktail is enhanced by performing heat mediated antigen retrieval using a high pH buffer. The HMB-45 portion of this antibody will stain with citrate buffer, pH 6.0; however, data shows the MART-1 portion of this cocktail may be negative unless a high pH buffer is used. We therefore, strongly recommend that you do not use citrate buffer for this antibody cocktail. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-Fr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Alternative names
Antigen LB39-AA, Antigen SK29-AA, MART-1, Melan A, Melan A protein, Melanoma antigen recognized by T-cells 1
Recommended products
Mouse Multiclonal Melanoma antibody. Suitable for Flow Cyt, IHC-P, IHC-Fr and reacts with Human samples. Cited in 29 publications.
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 97.5% Water, 0.99% BSA, 0.33% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.007% Hydrochloric acid- Form
- Liquid
- Clonality
- Multiclonal
- Clone number
- HMB45 + M2-7C10 + M2-9E3
- Purity
- Tissue culture supernatant
- Epitope
- HMB45 and MART-1
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
This cocktail is HMB45 + two different clones of MART-1[M2-7C10] + [M2-9E3]. HMB34 isotype: IgG1/kappa and both clones of MART-1: IgG2b + IgG2b
HMB-45 and MART-1 are coexpressed in the majority of melanomas, as well as uniquely expressed in certain cases. Thus, the HMB-45 and MART-1 cocktail is potentially more sensitive than HMB-45 or MART-1 alone. MART-1 is a cocktail of clones M2-7C10 and M2-9E3. The combination of HMB-45 and the MART-1 cocktail make this triple antibody cocktail a first-order pan melanoma screener.
This product was changed from ascites to tissue culture supernatant on 14/07/17. The following lots are from ascites and are still in stock as of 14/07/17 – GR313023 and GR3175007. Lot numbers higher than GR313023 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
What is an oligoclonal antibody?
This oligoclonal antibody is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [HMB45 + M2-7C10 + M2-9E3] (ab732), expandable thumbnail](https://content.abcam.com/products/images/melanoma-antibody-hmb45-m2-7c10-m2-9e3-ab732--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img23054.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [HMB45 + M2-7C10 + M2-9E3] (ab732)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [HMB45 + M2-7C10 + M2-9E3] (ab732)
ab732 staining Melanoma in Human lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
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