AB12502

Anti-Melanoma antibody [PNL2]

Name: Anti-Melanoma antibody [PNL2] (ab12502) | Abcam
Brand: Abcam Limited
SKU: AB12502
Rating: 4 (1 reviews)

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What is this?

(1 Review)

(7 Publications)

Mouse Monoclonal Melanoma antibody. Suitable for IHC-P and reacts with Human samples. Cited in 7 publications.

View Alternative Names

Antigen LB39-AA, Antigen SK29-AA, MART-1, Melan A, Melan A protein, Melanoma antigen recognized by T-cells 1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [PNL2] (AB12502)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [PNL2] (AB12502)

Formalin-fixed, paraffin-embedded human melanoma tissue stained for Melanoma using ab12502 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [PNL2] (AB12502)

IHC image of ab12502 staining in human melanoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab12502, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

PNL2

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Specificity

This antibody labels melanocytes in the cytoplasm and plasma membrane and is useful tool for the identification of melanomas and clear cell sarcomas. It seems to be a melanocyte antibody of autoimmune origin as it does not react with the non-melanocytic peptide antigen used for immunization.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.3 - 7.5 Preservative: 0.05% Sodium azide Constituents: Tissue culture supernatant, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Melanoma

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cells 13: PubMed38891124

2024

Establishment of Canine Oral Mucosal Melanoma Cell Lines and Their Xenogeneic Animal Models.

Applications

Unspecified application

Species

Unspecified reactive species

Shumin Li,Zichen Liu,Jinbao Lv,Di Lv,Huanming Xu,Hao Shi,Gang Liu,Degui Lin,Yipeng Jin

Veterinary and comparative oncology 22:388-397 PubMed38837514

2024

Pilot study to evaluate isolation by size of circulating tumour cells in canine oral melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Victor Nowosh,Alexcia Camila Braun,Anna Paula Carreta Ruano,Ludmilla Thomé Domingos Chinén,Cristina de Oliveira Massoco

Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc 35:168-172 PubMed36600502

2023

Invasive spindle-cell rhabdomyosarcoma with osteolysis in a dog: case report and literature review.

Applications

Unspecified application

Species

Unspecified reactive species

Junchao Shi,Rui Gao,Jing Zhang,Rongyi Xu,Qianhan Jia,Ying Ma,Huijun Lu,Kui Zhao,Feng Gao,Wenqi He

Journal of toxicologic pathology 34:161-180 PubMed33976473

2021

Databases for technical aspects of immunohistochemistry: 2021 update.

Applications

Unspecified application

Species

Unspecified reactive species

Takayasu Moroki,Saori Matsuo,Hirofumi Hatakeyama,Seigo Hayashi,Izumi Matsumoto,Satoshi Suzuki,Takashi Kotera,Kazuyoshi Kumagai,Kiyokazu Ozaki

Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc 30:315-318 PubMed29284381

2017

Biphasic malignant melanoma adenocarcinoma in the digit of a dog.

Applications

Unspecified application

Species

Unspecified reactive species

David B Needle,Olga Iglikova,Andrew D Miller

Journal of toxicologic pathology 30:79-107 PubMed28190929

2017

Databases for technical aspects of immunohistochemistry.

Applications

IHC

Species

Rat

Satoshi Furukawa,Mika Nagaike,Kiyokazu Ozaki

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 16:481-90 PubMed12748255

2003

PNL2, a new monoclonal antibody directed against a fixative-resistant melanocyte antigen.

Applications

Unspecified application

Species

Unspecified reactive species

Philippe Rochaix,Magali Lacroix-Triki,Laurence Lamant,Carole Pichereaux,Severine Valmary,Elena Puente,Talal Al Saati,Bernard Monsarrat,Christiane Susini,Louis Buscail,Georges Delsol,Jean-Jacques Voigt

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Melanoma antibody [PNL2]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [PNL2] (AB12502)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [PNL2] (AB12502)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Specificity

Alternative Products

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Secondary Antibodies

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Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (7)

Product promise