Anti-Melanoma gp100 antibody [EPR4864] (ab137062) is a rabbit monoclonal antibody detecting Melanoma gp100 in Western Blot, IHC-P. Suitable for Human.
- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications
Images
![Western blot - Anti-Melanoma gp100 antibody [EPR4864] (AB137062), expandable thumbnail](https://content.abcam.com/products/images/melanoma-gp100-antibody-epr4864-ab137062--western-blot-img82178.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Melanoma gp100 antibody [EPR4864] (AB137062)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (AB137062), expandable thumbnail](https://content.abcam.com/products/images/melanoma-gp100-antibody-epr4864-ab137062--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img77112.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (AB137062)")
![Western blot - Anti-Melanoma gp100 antibody [EPR4864] (AB137062), expandable thumbnail](https://content.abcam.com/products/images/melanoma-gp100-antibody-epr4864-ab137062--western-blot-img95071.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Melanoma gp100 antibody [EPR4864] (AB137062)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (AB137062), expandable thumbnail](https://content.abcam.com/products/images/melanoma-gp100-antibody-epr4864-ab137062--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img88619.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (AB137062)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (AB137062), expandable thumbnail](https://content.abcam.com/products/images/melanoma-gp100-antibody-epr4864-ab137062--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136073.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (AB137062)")
Publications
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- SLAS technology 29:1001062023A semi-automated pipeline for quantifying drusen-like deposits in human induced pluripotent stem cell-derived retinal pigment epithelium cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJenna Hall,Maciej Daniszewski,Shane Cheung,Kalyan Shobhana,Himeesh Kumar,Helena H Liang,Henry Beetham,Ellie Cho,Carla Abbott,Alex W Hewitt,Kaylene J Simpson,Robyn H Guymer,Daniel Paull,Alice Pébay,Grace E LidgerwoodPubMed 37657710
- Nature communications 13:42332022Transcriptomic and proteomic retinal pigment epithelium signatures of age-related macular degeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnne Senabouth,Maciej Daniszewski,Grace E Lidgerwood,Helena H Liang,Damián Hernández,Mehdi Mirzaei,Stacey N Keenan,Ran Zhang,Xikun Han,Drew Neavin,Louise Rooney,Maria Isabel G Lopez Sanchez,Lerna Gulluyan,Joao A Paulo,Linda Clarke,Lisa S Kearns,Vikkitharan Gnanasambandapillai,Chia-Ling Chan,Uyen Nguyen,Angela M Steinmann,Rachael A McCloy,Nona Farbehi,Vivek K Gupta,David A Mackey,Guy Bylsma,Nitin Verma,Stuart MacGregor,Matthew J Watt,Robyn H Guymer,Joseph E Powell,Alex W Hewitt,Alice PébayPubMed 35882847
- Regenerative therapy 19:24-342022Addition of Chk1 inhibitor and BMP4 cooperatively promotes retinal tissue formation in self-organizing human pluripotent stem cell differentiation culture.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSuguru Yamasaki,Atsushi Kuwahara,Akiyoshi Kishino,Toru Kimura,Masayo Takahashi,Michiko MandaiPubMed 35059477
- Scientific reports 11:77302021Identification of critical amino acid residues in the regulatory N-terminal domain of PMEL.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSusan M Mitchell,Morven Graham,Xinran Liu,Ralf M LeonhardtPubMed 33833328
- Methods in molecular biology (Clifton, N.J.) 2265:213-2222021Multi-Marker Immunomagnetic Enrichment of Circulating Melanoma Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAaron B Beasley,Emmanuel Acheampong,Weitao Lin,Elin S GrayPubMed 33704717
- Scientific reports 9:61012019Repeat domain-associated O-glycans govern PMEL fibrillar sheet architecture.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMorven Graham et. al.PubMed 30988362
- Cell cycle (Georgetown, Tex.) 17:844-8572018Degraded melanocores are incompetent to protect epidermal keratinocytes against UV damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWen-Juan Yi et. al.PubMed 29623762
- Investigative ophthalmology & visual science 59:1374-13832018Allogeneic iPSC-Derived RPE Cell Graft Failure Following Transplantation Into the Subretinal Space in Nonhuman Primates.Applications:Unspecified applicationReactive species:Macaque monkeyTrevor J McGill,Jonathan Stoddard,Lauren M Renner,Ilhem Messaoudi,Kapil Bharti,Shoukhrat Mitalipov,Andreas Lauer,David J Wilson,Martha NeuringerPubMed 29625461
- The Journal of investigative dermatology 138:171-1782017Melanocytes Sense Blue Light and Regulate Pigmentation through Opsin-3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesClaire Regazzetti,Laura Sormani,Delphine Debayle,Françoise Bernerd,Meri K Tulic,Gian Marco De Donatis,Bérengère Chignon-Sicard,Stéphane Rocchi,Thierry PasseronPubMed 28842328
- Translational vision science & technology 6:172017Long-Term Efficacy of GMP Grade Xeno-Free hESC-Derived RPE Cells Following Transplantation.Applications:IHC-PReactive species:RatTrevor J McGill,Osnat Bohana-Kashtan,Jonathan W Stoddard,Michael D Andrews,Neelay Pandit,Lior R Rosenberg-Belmaker,Ofer Wiser,Limor Matzrafi,Eyal Banin,Benjamin Reubinoff,Nir Netzer,Charles IrvingPubMed 28626601
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | WB | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/400 | Notes For unpurified, use 1/50 - 1/100. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes For unpurified, use 1/1000 - 1/2000. |
Associated Products
Select an associated product type
11 products for Alternative Product
Target data
Function
Forms physiological amyloids that play a central role in melanosome morphogenesis and pigmentation. The maturation of unpigmented premelanosomes from stage I to II is marked by assembly of processed amyloidogenic fragments into parallel fibrillar sheets, which elongate the vesicle into a striated ellipsoidal shape. In pigmented stage III and IV melanosomes, the amyloid matrix serves as a platform where eumelanin precursors accumulate at high local concentrations for pigment formation. May prevent pigmentation-associated toxicity by sequestering toxic reaction intermediates of eumelanin biosynthesis pathway. Represents a potent melanoma-specific antigen. Among melanoma non-mutated self-peptides, G9-154 (KTWGQYWQV), G9-209 (ITDQVPFSV) and G9-280 (YLEPGPVTA), appear to act as immunodominant common epitopes that stimulate anti-tumor immune response mediated by HLA-A-restricted cytotoxic T cells.
Alternative names
D12S53E, PMEL17, SILV, PMEL, Melanocyte protein PMEL, ME20-M, Melanocyte protein Pmel 17, Melanocytes lineage-specific antigen GP100, Melanoma-associated ME20 antigen, P1, P100, Premelanosome protein, Silver locus protein homolog, ME20M
Recommended products
Anti-Melanoma gp100 antibody [EPR4864] (ab137062) is a rabbit monoclonal antibody detecting Melanoma gp100 in Western Blot, IHC-P. Suitable for Human.
- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR4864
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
Notes
Anti-Melanoma gp100 antibody [EPR4864] (ab137062) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.
What is the molecular weight of Melanoma gp100?
Anti-Melanoma gp100 [EPR4864] (ab137062) specifically detects a band for Melanoma gp100 (UniProt: P40967) at a molecular weight of 70kDa.
Trusted by the scientific community
Anti-Melanoma gp100 [EPR4864] (ab137062) was first used in a scientific publication in 2012 and has been cited over 10 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EPR4864] also available for your convenience:
ab137062, Carrier free - Anti-Melanoma gp100 antibody [EPR4864] - BSA and Azide free ab190913, HRP - HRP Anti-Melanoma gp100 antibody [EPR4864] ab206094, APC - APC Anti-Melanoma gp100 antibody [EPR4864] ab319326, PE - PE Anti-Melanoma gp100 antibody [EPR4864] ab319463, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-Melanoma gp100 antibody [EPR4864] ab319583, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-Melanoma gp100 antibody [EPR4864] ab319689, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-Melanoma gp100 antibody [EPR4864] ab319819, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-Melanoma gp100 antibody [EPR4864] ab319960, Alexa Fluor® 750 - Alexa Fluor® 750 Anti-Melanoma gp100 antibody [EPR4864] ab321106
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Western blot - Anti-Melanoma gp100 antibody [EPR4864] (ab137062), expandable thumbnail](https://content.abcam.com/products/images/melanoma-gp100-antibody-epr4864-ab137062--western-blot-img82178.jpg "Western blot - Anti-Melanoma gp100 antibody [EPR4864] (ab137062)")
Western blot - Anti-Melanoma gp100 antibody [EPR4864] (ab137062)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Melanoma gp100 antibody [EPR4864] (ab137062) at 1/10000 dilution
All lanes: Human melanoma tissue lysate at 10 µg
SecondaryAll lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 70 kDa
Observed band size: 100 kDa, 26 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (ab137062), expandable thumbnail](https://content.abcam.com/products/images/melanoma-gp100-antibody-epr4864-ab137062--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img77112.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (ab137062)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (ab137062)
Immunohistochemical staining of paraffin embedded human melanoma with purified ab137062 at a working dilution of 1 in 400. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
![Western blot - Anti-Melanoma gp100 antibody [EPR4864] (ab137062), expandable thumbnail](https://content.abcam.com/products/images/melanoma-gp100-antibody-epr4864-ab137062--western-blot-img95071.jpg "Western blot - Anti-Melanoma gp100 antibody [EPR4864] (ab137062)")
Western blot - Anti-Melanoma gp100 antibody [EPR4864] (ab137062)
All lanes: Western blot - Anti-Melanoma gp100 antibody [EPR4864] (ab137062) at 1/1000 dilution
All lanes: Human melanoma tissue lysate at 10 µg
SecondaryAll lanes: HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 70 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (ab137062), expandable thumbnail](https://content.abcam.com/products/images/melanoma-gp100-antibody-epr4864-ab137062--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img88619.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (ab137062)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (ab137062)
Immunohistochemical staining of paraffin embedded human melanoma with unpurified ab137062 at a working dilution of 1 in 50. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (ab137062), expandable thumbnail](https://content.abcam.com/products/images/melanoma-gp100-antibody-epr4864-ab137062--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136073.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (ab137062)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (ab137062)
Immunohistochemical analysis using unpurified ab137062 showing negative staining in placenta tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (ab137062), expandable thumbnail](https://content.abcam.com/products/images/melanoma-gp100-antibody-epr4864-ab137062--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123410.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (ab137062)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (ab137062)
Immunohistochemical analysis using unpurifed ab137062 showing negative staining in normal skin tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (ab137062), expandable thumbnail](https://content.abcam.com/products/images/melanoma-gp100-antibody-epr4864-ab137062--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img98634.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (ab137062)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (ab137062)
Immunohistochemical analysis using unpurified ab137062 showing negative staining in normal kidney tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (ab137062), expandable thumbnail](https://content.abcam.com/products/images/melanoma-gp100-antibody-epr4864-ab137062--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img96169.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (ab137062)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (ab137062)
Immunohistochemical analysis using unpurified ab137062 showing negative staining in normal stomach tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (ab137062), expandable thumbnail](https://content.abcam.com/products/images/melanoma-gp100-antibody-epr4864-ab137062--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123410.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (ab137062)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (ab137062), expandable thumbnail](https://content.abcam.com/products/images/melanoma-gp100-antibody-epr4864-ab137062--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img98634.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (ab137062)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (ab137062), expandable thumbnail](https://content.abcam.com/products/images/melanoma-gp100-antibody-epr4864-ab137062--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img96169.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (ab137062)")
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