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AB137062

Anti-Melanoma gp100 antibody [EPR4864]

5

(1 Review)

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(11 Publications)

Anti-Melanoma gp100 antibody [EPR4864] (ab137062) is a rabbit monoclonal antibody detecting Melanoma gp100 in Western Blot, IHC-P. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

View Alternative Names

D12S53E, PMEL17, SILV, PMEL, Melanocyte protein PMEL, ME20-M, Melanocyte protein Pmel 17, Melanocytes lineage-specific antigen GP100, Melanoma-associated ME20 antigen, P1, P100, Premelanosome protein, Silver locus protein homolog, ME20M

8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (AB137062)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (AB137062)

Immunohistochemical staining of paraffin embedded human melanoma with purified ab137062 at a working dilution of 1 in 400. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (AB137062)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (AB137062)

Immunohistochemical staining of paraffin embedded human melanoma with unpurified ab137062 at a working dilution of 1 in 50. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

Western blot - Anti-Melanoma gp100 antibody [EPR4864] (AB137062)
  • WB

Unknown

Western blot - Anti-Melanoma gp100 antibody [EPR4864] (AB137062)

All lanes:

Western blot - Anti-Melanoma gp100 antibody [EPR4864] (ab137062) at 1/1000 dilution

All lanes:

Human melanoma tissue lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

Predicted band size: 70 kDa

false

Western blot - Anti-Melanoma gp100 antibody [EPR4864] (AB137062)
  • WB

Lab

Western blot - Anti-Melanoma gp100 antibody [EPR4864] (AB137062)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Melanoma gp100 antibody [EPR4864] (ab137062) at 1/10000 dilution

All lanes:

Human melanoma tissue lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 70 kDa

Observed band size: 100 kDa,26 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (AB137062)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (AB137062)

Immunohistochemical analysis using unpurified ab137062 showing negative staining in normal kidney tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (AB137062)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (AB137062)

Immunohistochemical analysis using unpurified ab137062 showing negative staining in normal stomach tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (AB137062)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (AB137062)

Immunohistochemical analysis using unpurified ab137062 showing negative staining in placenta tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (AB137062)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] (AB137062)

Immunohistochemical analysis using unpurifed ab137062 showing negative staining in normal skin tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • HRP

    HRP Anti-Melanoma gp100 antibody [EPR4864]

  • Carrier free

    Anti-Melanoma gp100 antibody [EPR4864] - BSA and Azide free

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Melanoma gp100 antibody [EPR4864]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Melanoma gp100 antibody [EPR4864]

  • 578 PE

    PE Anti-Melanoma gp100 antibody [EPR4864]

  • 660 APC

    APC Anti-Melanoma gp100 antibody [EPR4864]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Melanoma gp100 antibody [EPR4864]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Melanoma gp100 antibody [EPR4864]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Melanoma gp100 antibody [EPR4864]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4864

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

AB237898

Anti-Melanoma gp100 antibody [PMEL/2038] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [PMEL/2038] - BSA and Azide free (AB237898)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [HMB45] - BSA and Azide free (AB212829)

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Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [PMEL/2038] (AB238048)

  • 0
  • 0
Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [HMB45] (AB787)

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Primary Antibodies

AB205469

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Melanoma gp100 antibody [EP4863(2)] (AB205469)

  • 0
  • 0
Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Melanoma gp100 antibody [EP4863(2)] (AB204674)

  • 0
  • 0
Primary Antibodies

AB137078

Anti-Melanoma gp100 antibody [EP4863(2)]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EP4863(2)] (AB137078)

  • 5
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Primary Antibodies

AB193201

Anti-Melanoma gp100 antibody [EP4863(2)] - Low endotoxin, Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EP4863(2)] - Low endotoxin, Azide free (AB193201)

  • 0
  • 0
Primary Antibodies

AB238047

Anti-Melanoma gp100 antibody [PMEL/2037]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [PMEL/2037] (AB238047)

  • 0
  • 0
Primary Antibodies

AB246730

Alexa Fluor® 647 Anti-Melanoma gp100 antibody [EP4863(2)]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Melanoma gp100 antibody [EP4863(2)] (AB246730)

  • 0
  • 0
Primary Antibodies

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Anti-Melanoma gp100 antibody [EP4863(2)] - BSA and Azide free

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EP4863(2)] - BSA and Azide free (AB256497)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
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Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [EPR20380] (AB210546)

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Primary Antibodies

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Primary Antibodies

AB205921

Anti-PD-L1 antibody [28-8]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (AB205921)

  • 4
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/400", "IHCP-species-notes": "<p><strong>For unpurified, use 1/50 - 1/100.</strong></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p><strong>For unpurified, use 1/1000 - 1/2000.</strong></p>" } } }
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Product details

What is this antibody validated in?
Anti-Melanoma gp100 antibody [EPR4864] (ab137062) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of Melanoma gp100?
Anti-Melanoma gp100 [EPR4864] (ab137062) specifically detects a band for Melanoma gp100 (UniProt: P40967) at a molecular weight of 70kDa.

Trusted by the scientific community
Anti-Melanoma gp100 [EPR4864] (ab137062) was first used in a scientific publication in 2012 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR4864] also available for your convenience: ab137062, Carrier free - ab190913, HRP - ab206094, APC - ab319326, PE - ab319463, Alexa Fluor® 488 - ab319583, Alexa Fluor® 647 - ab319689, Alexa Fluor® 594 - ab319819, Alexa Fluor® 555 - ab319960, Alexa Fluor® 750 - ab321106

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Forms physiological amyloids that play a central role in melanosome morphogenesis and pigmentation. The maturation of unpigmented premelanosomes from stage I to II is marked by assembly of processed amyloidogenic fragments into parallel fibrillar sheets, which elongate the vesicle into a striated ellipsoidal shape. In pigmented stage III and IV melanosomes, the amyloid matrix serves as a platform where eumelanin precursors accumulate at high local concentrations for pigment formation. May prevent pigmentation-associated toxicity by sequestering toxic reaction intermediates of eumelanin biosynthesis pathway.. Represents a potent melanoma-specific antigen. Among melanoma non-mutated self-peptides, G9-154 (KTWGQYWQV), G9-209 (ITDQVPFSV) and G9-280 (YLEPGPVTA), appear to act as immunodominant common epitopes that stimulate anti-tumor immune response mediated by HLA-A-restricted cytotoxic T cells.
See full target information PMEL
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

SLAS technology 29:100106 PubMed37657710

2023

A semi-automated pipeline for quantifying drusen-like deposits in human induced pluripotent stem cell-derived retinal pigment epithelium cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jenna Hall,Maciej Daniszewski,Shane Cheung,Kalyan Shobhana,Himeesh Kumar,Helena H Liang,Henry Beetham,Ellie Cho,Carla Abbott,Alex W Hewitt,Kaylene J Simpson,Robyn H Guymer,Daniel Paull,Alice Pébay,Grace E Lidgerwood

Nature communications 13:4233 PubMed35882847

2022

Transcriptomic and proteomic retinal pigment epithelium signatures of age-related macular degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Anne Senabouth,Maciej Daniszewski,Grace E Lidgerwood,Helena H Liang,Damián Hernández,Mehdi Mirzaei,Stacey N Keenan,Ran Zhang,Xikun Han,Drew Neavin,Louise Rooney,Maria Isabel G Lopez Sanchez,Lerna Gulluyan,Joao A Paulo,Linda Clarke,Lisa S Kearns,Vikkitharan Gnanasambandapillai,Chia-Ling Chan,Uyen Nguyen,Angela M Steinmann,Rachael A McCloy,Nona Farbehi,Vivek K Gupta,David A Mackey,Guy Bylsma,Nitin Verma,Stuart MacGregor,Matthew J Watt,Robyn H Guymer,Joseph E Powell,Alex W Hewitt,Alice Pébay

Regenerative therapy 19:24-34 PubMed35059477

2022

Addition of Chk1 inhibitor and BMP4 cooperatively promotes retinal tissue formation in self-organizing human pluripotent stem cell differentiation culture.

Applications

Unspecified application

Species

Unspecified reactive species

Suguru Yamasaki,Atsushi Kuwahara,Akiyoshi Kishino,Toru Kimura,Masayo Takahashi,Michiko Mandai

Scientific reports 11:7730 PubMed33833328

2021

Identification of critical amino acid residues in the regulatory N-terminal domain of PMEL.

Applications

Unspecified application

Species

Unspecified reactive species

Susan M Mitchell,Morven Graham,Xinran Liu,Ralf M Leonhardt

Methods in molecular biology (Clifton, N.J.) 2265:213-222 PubMed33704717

2021

Multi-Marker Immunomagnetic Enrichment of Circulating Melanoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Aaron B Beasley,Emmanuel Acheampong,Weitao Lin,Elin S Gray

Scientific reports 9:6101 PubMed30988362

2019

Repeat domain-associated O-glycans govern PMEL fibrillar sheet architecture.

Applications

Unspecified application

Species

Unspecified reactive species

Morven Graham,Athanasia C Tzika,Susan M Mitchell,Xinran Liu,Ralf M Leonhardt

Cell cycle (Georgetown, Tex.) 17:844-857 PubMed29623762

2018

Degraded melanocores are incompetent to protect epidermal keratinocytes against UV damage.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Juan Yi,Meng-Yun Su,Ying Shi,Shan Jiang,Shi-Zheng Xu,Tie-Chi Lei

Investigative ophthalmology & visual science 59:1374-1383 PubMed29625461

2018

Allogeneic iPSC-Derived RPE Cell Graft Failure Following Transplantation Into the Subretinal Space in Nonhuman Primates.

Applications

Unspecified application

Species

Macaque monkey

Trevor J McGill,Jonathan Stoddard,Lauren M Renner,Ilhem Messaoudi,Kapil Bharti,Shoukhrat Mitalipov,Andreas Lauer,David J Wilson,Martha Neuringer

The Journal of investigative dermatology 138:171-178 PubMed28842328

2017

Melanocytes Sense Blue Light and Regulate Pigmentation through Opsin-3.

Applications

Unspecified application

Species

Unspecified reactive species

Claire Regazzetti,Laura Sormani,Delphine Debayle,Françoise Bernerd,Meri K Tulic,Gian Marco De Donatis,Bérengère Chignon-Sicard,Stéphane Rocchi,Thierry Passeron

Translational vision science & technology 6:17 PubMed28626601

2017

Long-Term Efficacy of GMP Grade Xeno-Free hESC-Derived RPE Cells Following Transplantation.

Applications

IHC-P

Species

Rat

Trevor J McGill,Osnat Bohana-Kashtan,Jonathan W Stoddard,Michael D Andrews,Neelay Pandit,Lior R Rosenberg-Belmaker,Ofer Wiser,Limor Matzrafi,Eyal Banin,Benjamin Reubinoff,Nir Netzer,Charles Irving
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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