Rabbit Monoclonal Melanoma gp100 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] - BSA and Azide free (AB190913), expandable thumbnail](https://content.abcam.com/products/images/melanoma-gp100-antibody-epr4864-bsa-and-azide-free-ab190913--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img77112.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] - BSA and Azide free (AB190913)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] - BSA and Azide free (AB190913), expandable thumbnail](https://content.abcam.com/products/images/melanoma-gp100-antibody-epr4864-bsa-and-azide-free-ab190913--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img88619.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] - BSA and Azide free (AB190913)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] - BSA and Azide free (AB190913), expandable thumbnail](https://content.abcam.com/products/images/melanoma-gp100-antibody-epr4864-bsa-and-azide-free-ab190913--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136073.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] - BSA and Azide free (AB190913)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] - BSA and Azide free (AB190913), expandable thumbnail](https://content.abcam.com/products/images/melanoma-gp100-antibody-epr4864-bsa-and-azide-free-ab190913--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123410.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] - BSA and Azide free (AB190913)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] - BSA and Azide free (AB190913), expandable thumbnail](https://content.abcam.com/products/images/melanoma-gp100-antibody-epr4864-bsa-and-azide-free-ab190913--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img98634.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] - BSA and Azide free (AB190913)")
Publications
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- Oncology letters 26:4092023Renal epithelioid angiomyolipoma: A case report.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongyun Li,Xuebei Zhang,Qingli Zhao,Jin Wang,Xiaoqing Yang,Shengliang HuangPubMed 37600337
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | WB | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
11 products for Alternative Product
1 product for Alternative Version
Target data
Function
Forms physiological amyloids that play a central role in melanosome morphogenesis and pigmentation. The maturation of unpigmented premelanosomes from stage I to II is marked by assembly of processed amyloidogenic fragments into parallel fibrillar sheets, which elongate the vesicle into a striated ellipsoidal shape. In pigmented stage III and IV melanosomes, the amyloid matrix serves as a platform where eumelanin precursors accumulate at high local concentrations for pigment formation. May prevent pigmentation-associated toxicity by sequestering toxic reaction intermediates of eumelanin biosynthesis pathway. Represents a potent melanoma-specific antigen. Among melanoma non-mutated self-peptides, G9-154 (KTWGQYWQV), G9-209 (ITDQVPFSV) and G9-280 (YLEPGPVTA), appear to act as immunodominant common epitopes that stimulate anti-tumor immune response mediated by HLA-A-restricted cytotoxic T cells.
Alternative names
D12S53E, PMEL17, SILV, PMEL, Melanocyte protein PMEL, ME20-M, Melanocyte protein Pmel 17, Melanocytes lineage-specific antigen GP100, Melanoma-associated ME20 antigen, P1, P100, Premelanosome protein, Silver locus protein homolog, ME20M
Recommended products
Rabbit Monoclonal Melanoma gp100 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR4864
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab190913 is the carrier-free version of Anti-Melanoma gp100 antibody [EPR4864] ab137062.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] - BSA and Azide free (ab190913), expandable thumbnail](https://content.abcam.com/products/images/melanoma-gp100-antibody-epr4864-bsa-and-azide-free-ab190913--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img77112.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] - BSA and Azide free (ab190913)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] - BSA and Azide free (ab190913)
Immunohistochemical staining of paraffin embedded human melanoma with purified Anti-Melanoma gp100 antibody [EPR4864] ab137062 at a working dilution of 1 in 400. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Melanoma gp100 antibody [EPR4864] ab137062).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] - BSA and Azide free (ab190913), expandable thumbnail](https://content.abcam.com/products/images/melanoma-gp100-antibody-epr4864-bsa-and-azide-free-ab190913--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img88619.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] - BSA and Azide free (ab190913)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] - BSA and Azide free (ab190913)
Immunohistochemical staining of paraffin embedded human melanoma with unpurified Anti-Melanoma gp100 antibody [EPR4864] ab137062 at a working dilution of 1 in 50. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Melanoma gp100 antibody [EPR4864] ab137062).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] - BSA and Azide free (ab190913), expandable thumbnail](https://content.abcam.com/products/images/melanoma-gp100-antibody-epr4864-bsa-and-azide-free-ab190913--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136073.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] - BSA and Azide free (ab190913)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] - BSA and Azide free (ab190913)
Immunohistochemical analysis using unpurified Anti-Melanoma gp100 antibody [EPR4864] ab137062 showing negative staining in placenta tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Melanoma gp100 antibody [EPR4864] ab137062).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] - BSA and Azide free (ab190913), expandable thumbnail](https://content.abcam.com/products/images/melanoma-gp100-antibody-epr4864-bsa-and-azide-free-ab190913--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123410.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] - BSA and Azide free (ab190913)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] - BSA and Azide free (ab190913)
Immunohistochemical analysis using unpurifed Anti-Melanoma gp100 antibody [EPR4864] ab137062 showing negative staining in normal skin tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Melanoma gp100 antibody [EPR4864] ab137062).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] - BSA and Azide free (ab190913), expandable thumbnail](https://content.abcam.com/products/images/melanoma-gp100-antibody-epr4864-bsa-and-azide-free-ab190913--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img98634.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] - BSA and Azide free (ab190913)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] - BSA and Azide free (ab190913)
Immunohistochemical analysis using unpurified Anti-Melanoma gp100 antibody [EPR4864] ab137062 showing negative staining in normal kidney tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Melanoma gp100 antibody [EPR4864] ab137062).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] - BSA and Azide free (ab190913), expandable thumbnail](https://content.abcam.com/products/images/melanoma-gp100-antibody-epr4864-bsa-and-azide-free-ab190913--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img96169.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] - BSA and Azide free (ab190913)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] - BSA and Azide free (ab190913)
Immunohistochemical analysis using unpurified Anti-Melanoma gp100 antibody [EPR4864] ab137062 showing negative staining in normal stomach tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Melanoma gp100 antibody [EPR4864] ab137062).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] - BSA and Azide free (ab190913), expandable thumbnail](https://content.abcam.com/products/images/melanoma-gp100-antibody-epr4864-bsa-and-azide-free-ab190913--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img96169.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EPR4864] - BSA and Azide free (ab190913)")
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