AB787

Anti-Melanoma gp100 antibody [HMB45]

Name: Anti-Melanoma gp100 antibody [HMB45] Mouse monoclonal (ab787) | Abcam
Brand: Abcam Limited
SKU: AB787
Price: 520 USD
Availability: InStock
Rating: 3 (3 reviews)

(3 Reviews)

(43 Publications)

Mouse Monoclonal Melanoma gp100 antibody. Suitable for Flow Cyt, IHC-P, ICC/IF and reacts with Human samples. Cited in 43 publications. Immunogen corresponding to Cell preparation containing PMEL protein.

View Alternative Names

D12S53E, PMEL17, SILV, PMEL, Melanocyte protein PMEL, ME20-M, Melanocyte protein Pmel 17, Melanocytes lineage-specific antigen GP100, Melanoma-associated ME20 antigen, P1, P100, Premelanosome protein, Silver locus protein homolog, ME20M

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [HMB45] (AB787)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [HMB45] (AB787)

Formalin-fixed, paraffin-embedded human testis tissue stained with ab787 at 1 μg/ml in immunohistochemical analysis.

IHC-P

AbReview17800****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [HMB45] (AB787)

ab787 staining Melanoma in the human Melanoma cell line WM3248 xenopraph by IHC-P (formaldehyde-fixed paraffin-embedded sections). Tissue samples were fixed with formaldehyde; permeabilized with 0.1% Triton X-100 and blocked with 100% Dakocytomation X0909 for 1 hour at room temperature; antigen retrival was by heat mediation in Citric buffer (pH6). The sample was incubated with primary antibody (1/25) at 4°C for 18 hours. An HRP-conjugated Goat polyclonal to mouse IgG (1/100) was used as secondary antibody.

This image is courtesy of an Abreview submitted by Dr. Hongwei Shao

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [HMB45] (AB787)

Formalin-fixed, paraffin-embedded human melanoma tissue stained with ab787 at 1 μg/ml in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [HMB45] (AB787)

ab787 - immunohistochemistry

Formalin fixed paraffin embedded human melanoma stained with ab787, using ABC and AEC chromogen.

Flow Cytometry - Anti-Melanoma gp100 antibody [HMB45] (AB787)

Flow Cyt

Unknown

Flow Cytometry - Anti-Melanoma gp100 antibody [HMB45] (AB787)

Overlay histogram showing Malme-3 cells stained with ab787 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab787, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Malme-3 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HMB45

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, Flow Cyt

applications

Immunogen

Cell preparation containing PMEL protein. The exact immunogen used to generate this antibody is proprietary information.

P40967

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/10", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "Incubate for 30 minutes at room temperature. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/10", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "Incubate for 30 minutes at room temperature. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/10", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "Incubate for 30 minutes at room temperature. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: Tissue culture supernatant, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Forms physiological amyloids that play a central role in melanosome morphogenesis and pigmentation. The maturation of unpigmented premelanosomes from stage I to II is marked by assembly of processed amyloidogenic fragments into parallel fibrillar sheets, which elongate the vesicle into a striated ellipsoidal shape. In pigmented stage III and IV melanosomes, the amyloid matrix serves as a platform where eumelanin precursors accumulate at high local concentrations for pigment formation. May prevent pigmentation-associated toxicity by sequestering toxic reaction intermediates of eumelanin biosynthesis pathway.. Represents a potent melanoma-specific antigen. Among melanoma non-mutated self-peptides, G9-154 (KTWGQYWQV), G9-209 (ITDQVPFSV) and G9-280 (YLEPGPVTA), appear to act as immunodominant common epitopes that stimulate anti-tumor immune response mediated by HLA-A-restricted cytotoxic T cells.

See full target information PMEL

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Publications (43)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Nucleic acids 35:102357 PubMed39618825

2024

Local CpG- siRNA treatment improves antitumor effects of immune checkpoint inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Chunyan Zhang,Rui Huang,Lyuzhi Ren,Antons Martincuks,JiEun Song,Marcin Kortylewski,Piotr Swiderski,Stephen J Forman,Hua Yu

Investigative ophthalmology & visual science 65:7 PubMed38833258

2024

Radiation-Induced DNA Damage in Uveal Melanoma Is Influenced by Dose Delivery and Chromosome 3 Status.

Applications

Unspecified application

Species

Unspecified reactive species

Aysegül Tura,Yingda Zhu,Siranush Vardanyan,Michelle Prasuhn,Vinodh Kakkassery,Julia Lüke,Hartmut Merz,Frank Paulsen,Dirk Rades,Florian Cremers,Karl-Ulrich Bartz-Schmidt,Salvatore Grisanti

Proceedings of the National Academy of Sciences of the United States of America 121:e2321323121 PubMed38607931

2024

Extracellular vesicles released by keratinocytes regulate melanosome maturation, melanocyte dendricity, and pigment transfer.

Applications

Unspecified application

Species

Unspecified reactive species

Marie-Thérèse Prospéri,Cécile Giordano,Mireia Gomez-Duro,Ilse Hurbain,Anne-Sophie Macé,Graça Raposo,Gisela D'Angelo

Nature communications 14:7759 PubMed38030596

2023

HDAC8-mediated inhibition of EP300 drives a transcriptional state that increases melanoma brain metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Michael F Emmons,Richard L Bennett,Alberto Riva,Kanchan Gupta,Larissa Anastasio Da Costa Carvalho,Chao Zhang,Robert Macaulay,Daphne Dupéré-Richér,Bin Fang,Edward Seto,John M Koomen,Jiannong Li,Y Ann Chen,Peter A Forsyth,Jonathan D Licht,Keiran S M Smalley

Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) 29:e13345 PubMed37231929

2023

The effects of miRNA-27a-3p on human epidermal melanocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Li,Suqin Wang,Jing Ning,Xiaoqian Mao,Kang Ge,Ruzhi Zhang

International journal of molecular sciences 24: PubMed37175529

2023

Efficient Isolation and Expansion of Limbal Melanocytes for Tissue Engineering.

Applications

Unspecified application

Species

Unspecified reactive species

Naresh Polisetti,Thomas Reinhard,Günther Schlunck

The Journal of investigative dermatology 143:317-327.e6 PubMed36063887

2022

S-Palmitoylation of Tyrosinase at Cysteine Regulates Melanogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yoko Niki,Naoko Adachi,Masaki Fukata,Yuko Fukata,Shinichiro Oku,Chieko Makino-Okamura,Seiji Takeuchi,Kazumasa Wakamatsu,Shosuke Ito,Lieve Declercq,Daniel B Yarosh,Tomas Mammone,Chikako Nishigori,Naoaki Saito,Takehiko Ueyama

Frontiers in oncology 11:644592 PubMed34178631

2021

Normalization of Enzyme Expression and Activity Regulating Vitamin A Metabolism Increases RAR-Beta Expression and Reduces Cellular Migration and Proliferation in Diseases Caused by Tuberous Sclerosis Gene Mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Elhusseiny Mohamed Mahmoud Abdelwahab,Judit Bovari-Biri,Gabor Smuk,Tunde Harko,Janos Fillinger,Judit Moldvay,Vera P Krymskaya,Judit E Pongracz

Cell and tissue banking 22:67-75 PubMed32978700

2020

Dedifferentiation of human epidermal melanocytes in vitro by long-term trypsinization.

Applications

Unspecified application

Species

Unspecified reactive species

Ren-He Chen,Li Xiao,Ru-Zhi Zhang,Sheng-Yi Wang,Yue Li

Nature communications 11:2988 PubMed32532976

2020

Coupling of melanocyte signaling and mechanics by caveolae is required for human skin pigmentation.

Applications

Unspecified application

Species

Unspecified reactive species

Lia Domingues,Ilse Hurbain,Floriane Gilles-Marsens,Julia Sirés-Campos,Nathalie André,Melissa Dewulf,Maryse Romao,Christine Viaris de Lesegno,Anne-Sophie Macé,Cédric Blouin,Christelle Guéré,Katell Vié,Graça Raposo,Christophe Lamaze,Cédric Delevoye

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Melanoma gp100 antibody [HMB45]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [HMB45] (AB787)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [HMB45] (AB787)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [HMB45] (AB787)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [HMB45] (AB787)

Flow Cytometry - Anti-Melanoma gp100 antibody [HMB45] (AB787)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

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Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (43)

Product promise