AB212829

Anti-Melanoma gp100 antibody [HMB45] - BSA and Azide free

Name: Anti-Melanoma gp100 antibody [HMB45] - BSA and Azide free (ab212829) | Abcam
Brand: Abcam Limited
SKU: AB212829

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(3 Publications)

Mouse Monoclonal Melanoma gp100 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Cell preparation containing PMEL protein.

View Alternative Names

D12S53E, PMEL17, SILV, PMEL, Melanocyte protein PMEL, ME20-M, Melanocyte protein Pmel 17, Melanocytes lineage-specific antigen GP100, Melanoma-associated ME20 antigen, P1, P100, Premelanosome protein, Silver locus protein homolog, ME20M

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [HMB45] - BSA and Azide free (AB212829)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [HMB45] - BSA and Azide free (AB212829)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human melanoma tissue labeling Melanoma gp100 with ab212829 at 1 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [HMB45] - BSA and Azide free (AB212829)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue labeling Melanoma gp100 with ab212829 at 1 μg/ml.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HMB45

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Cell preparation containing PMEL protein. The exact immunogen used to generate this antibody is proprietary information.

P40967

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "(Primary incubation for 30 minutes at RT)." }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "(Primary incubation for 30 minutes at RT)." }, "Dog": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "(Primary incubation for 30 minutes at RT)." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

ab212829 was purified from Bioreactor Concentrate by Protein A/G.

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Forms physiological amyloids that play a central role in melanosome morphogenesis and pigmentation. The maturation of unpigmented premelanosomes from stage I to II is marked by assembly of processed amyloidogenic fragments into parallel fibrillar sheets, which elongate the vesicle into a striated ellipsoidal shape. In pigmented stage III and IV melanosomes, the amyloid matrix serves as a platform where eumelanin precursors accumulate at high local concentrations for pigment formation. May prevent pigmentation-associated toxicity by sequestering toxic reaction intermediates of eumelanin biosynthesis pathway.. Represents a potent melanoma-specific antigen. Among melanoma non-mutated self-peptides, G9-154 (KTWGQYWQV), G9-209 (ITDQVPFSV) and G9-280 (YLEPGPVTA), appear to act as immunodominant common epitopes that stimulate anti-tumor immune response mediated by HLA-A-restricted cytotoxic T cells.

See full target information PMEL

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

PloS one 19:e0307577 PubMed39739680

2025

Immune cell infiltration and modulation of the blood-brain barrier in a guinea pig model of tuberculosis: Observations without evidence of bacterial dissemination to the brain.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda S Latham,Charlize E Geer,David F Ackart,Kristin N Weninger,Chase C Gross,Brendan K Podell,Randall J Basaraba,Julie A Moreno

Experimental and therapeutic medicine 24:631 PubMed36160893

2022

Mediastinal cavernous angioleiomyoma: A case report and review of literature.

Applications

Unspecified application

Species

Unspecified reactive species

Zhibo Zuo,Wanxin Wu,Xin Li,Lin Zhang,Jingyu Wang,Zhiqin Guo,Shaoqing Hu,Qi Zhang

Cancer communications (London, England) 42:627-647 PubMed35666052

2022

Candidate therapeutic agents in a newly established triple wild-type mucosal melanoma cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Chaoji Shi,Ziyue Gu,Shengming Xu,Houyu Ju,Yunteng Wu,Yong Han,Jiayi Li,Chuwen Li,Jing Wu,Lizhen Wang,Jiang Li,Guoyu Zhou,Weimin Ye,Guoxin Ren,Zhiyuan Zhang,Rong Zhou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Melanoma gp100 antibody [HMB45] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [HMB45] - BSA and Azide free (AB212829)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [HMB45] - BSA and Azide free (AB212829)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

Product promise