Mouse Monoclonal Melanoma gp100 antibody. Carrier free. Suitable for IHC-P, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PMEL aa 350-550.
Images
![Protein Array - Anti-Melanoma gp100 antibody [PMEL/2039] - BSA and Azide free (AB237899), expandable thumbnail](https://content.abcam.com/products/images/melanoma-gp100-antibody-pmel-2039-bsa-and-azide-free-ab237899--protein-array-img141613.jpg/_jcr_content/renditions/webp-475.webp "Protein Array - Anti-Melanoma gp100 antibody [PMEL/2039] - BSA and Azide free (AB237899)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [PMEL/2039] - BSA and Azide free (AB237899), expandable thumbnail](https://content.abcam.com/products/images/melanoma-gp100-antibody-pmel-2039-bsa-and-azide-free-ab237899--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123687.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [PMEL/2039] - BSA and Azide free (AB237899)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [PMEL/2039] - BSA and Azide free (AB237899), expandable thumbnail](https://content.abcam.com/products/images/melanoma-gp100-antibody-pmel-2039-bsa-and-azide-free-ab237899--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111407.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [PMEL/2039] - BSA and Azide free (AB237899)")
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Recombinant Fragment Protein within Human PMEL aa 350-550. The exact immunogen used to generate this antibody is proprietary information. Database link P40967
Reactivity data
Select an application| IHC-P | Protein Array | |
|---|---|---|
| Human | Tested | Expected |
| Recombinant full length protein - Human | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes Incubate with primary antibody for 30 minutes at room temperature. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Protein Array
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
1 products for Alternative Version
Target data
Function
Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity.
Alternative names
Melanocyte protein PMEL, ME20-M, Melanocyte protein Pmel 17, Melanocytes lineage-specific antigen GP100, Melanoma-associated ME20 antigen, P1, P100, Premelanosome protein, Silver locus protein homolog, ME20M, SILV, PMEL17, D12S53E, PMEL
Recommended products
Mouse Monoclonal Melanoma gp100 antibody. Carrier free. Suitable for IHC-P, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PMEL aa 350-550.
Alternative names
Melanocyte protein PMEL, ME20-M, Melanocyte protein Pmel 17, Melanocytes lineage-specific antigen GP100, Melanoma-associated ME20 antigen, P1, P100, Premelanosome protein, Silver locus protein homolog, ME20M, SILV, PMEL17, D12S53E, PMEL
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human PMEL aa 350-550. The exact immunogen used to generate this antibody is proprietary information. Database link P40967
- Carrier free
Yes
- Clone number
PMEL/2039
- Purification technique
Affinity purification Protein A/G
- Light chain type
kappa
- Concentration
- Purification notes
Purified from bioreactor concentrate by Protein A/G.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Protein Array - Anti-Melanoma gp100 antibody [PMEL/2039] - BSA and Azide free (ab237899), expandable thumbnail](https://content.abcam.com/products/images/melanoma-gp100-antibody-pmel-2039-bsa-and-azide-free-ab237899--protein-array-img141613.jpg "Protein Array - Anti-Melanoma gp100 antibody [PMEL/2039] - BSA and Azide free (ab237899)")
Protein Array - Anti-Melanoma gp100 antibody [PMEL/2039] - BSA and Azide free (ab237899)
This data was produced with Anti-Melanoma gp100 antibody [PMEL/2039] ab238049, the same antibody in a different formulation with BSA and Azide.
Anti-Melanoma gp100 antibody [PMEL/2039] ab238049 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [PMEL/2039] - BSA and Azide free (ab237899), expandable thumbnail](https://content.abcam.com/products/images/melanoma-gp100-antibody-pmel-2039-bsa-and-azide-free-ab237899--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123687.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [PMEL/2039] - BSA and Azide free (ab237899)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [PMEL/2039] - BSA and Azide free (ab237899)
Formalin-fixed, paraffin-embedded human melanoma tissue stained for Melanoma gp100 using ab237899 at 2 μg/ml in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [PMEL/2039] - BSA and Azide free (ab237899), expandable thumbnail](https://content.abcam.com/products/images/melanoma-gp100-antibody-pmel-2039-bsa-and-azide-free-ab237899--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111407.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [PMEL/2039] - BSA and Azide free (ab237899)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [PMEL/2039] - BSA and Azide free (ab237899)
Formalin-fixed, paraffin-embedded human skin tissue stained for Melanoma gp100 using ab237899 at 2 μg/ml in immunohistochemical analysis.
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