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AB166932

Anti-Melanoma Inhibitory Activity antibody

5

(2 Reviews)

|

(3 Publications)

Goat Polyclonal Melanoma Inhibitory Activity antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human MIA aa 1-50.

View Alternative Names

Melanoma-derived growth regulatory protein, Melanoma inhibitory activity protein, MIA

1 Images
Western blot - Anti-Melanoma Inhibitory Activity antibody (AB166932)
  • WB

Unknown

Western blot - Anti-Melanoma Inhibitory Activity antibody (AB166932)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-Melanoma Inhibitory Activity antibody (ab166932) at 1 µg

All lanes:

Human Glioma lysates (in RIPA buffer) at 35 µg

Predicted band size: 15 kDa

true

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human MIA aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q16674

Specificity

The immunizing peptide represents the N terminus of the mature protein. Reported variants represent identical protein: NP_006524.1, NP_001189482.1.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab166932 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.
See full target information MIA

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Neoplasma 67:660-667 PubMed31986890

2020

The clinical significance of MIA gene in tumorigenesis of lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Q H Gu,D Li,Z H Xie,Q B Shen

Oncotarget 10:6079-6095 PubMed31692760

2019

Targeted photodynamic therapy treatment of A375 metastatic melanoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Channay Naidoo,Cherie Ann Kruger,Heidi Abrahamse

Molecular and cellular biology 35:1238-53 PubMed25605336

2015

Muscle-derived extracellular signal-regulated kinases 1 and 2 are required for the maintenance of adult myofibers and their neuromuscular junctions.

Applications

Unspecified application

Species

Unspecified reactive species

Bonnie Seaberg,Gabrielle Henslee,Shuo Wang,Ximena Paez-Colasante,Gary E Landreth,Mendell Rimer
View all publications

Product promise

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