AB186731

Anti-Melanoma Inhibitory Activity antibody [EPR15597]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal Melanoma Inhibitory Activity antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications.

View Alternative Names

Melanoma-derived growth regulatory protein, Melanoma inhibitory activity protein, MIA

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma Inhibitory Activity antibody [EPR15597] (AB186731)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma Inhibitory Activity antibody [EPR15597] (AB186731)

Immunohistochemical analysis of paraffin-embedded Human melanoma tissue labeling Melanoma Inhibitory Activity with ab186731 at 1/100 dilution. A Ready to use HRP Polymer for Rabbit IgG was used as secondary. Counterstain : Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-Melanoma Inhibitory Activity antibody [EPR15597] (AB186731)

All lanes:

Western blot - Anti-Melanoma Inhibitory Activity antibody [EPR15597] (ab186731) at 1/1000 dilution

Lane 1:

WM-266-4 cell lysate at 20 µg

Lane 2:

A375 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 100 kDa,15 kDa,47 kDa,49 kDa

Observed band size: 47 kDa

false

Carrier free

Anti-Melanoma Inhibitory Activity antibody [EPR15597] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15597

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.

See full target information MIA

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 23:227 PubMed40001145

2025

Revolutionizing the treatment of intervertebral disc degeneration: an approach based on molecular typing.

Applications

Unspecified application

Species

Unspecified reactive species

Shaofeng Chen,Wei Zhang,Yifan Liu,Runzhi Huang,Xiaoyi Zhou,Xianzhao Wei

International journal of molecular sciences 22: PubMed33810045

2021

Sesquiterpene Lactone Deoxyelephantopin Isolated from and Its Derivative DETD-35 Suppress BRAF Mutant Melanoma Lung Metastasis in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Biljana Cvetanova,Meng-Yi Li,Chung-Chih Yang,Pei-Wen Hsiao,Yu-Chih Yang,Jia-Hua Feng,Ya-Ching Shen,Kyoko Nakagawa-Goto,Kuo-Hsiung Lee,Lie-Fen Shyur

International journal of molecular sciences 20: PubMed31689918

2019

Lack of Delta-Sarcoglycan () Results in Retinal Degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Andric C Perez-Ortiz,Martha J Peralta-Ildefonso,Esmeralda Lira-Romero,Ernesto Moya-Albor,Jorge Brieva,Israel Ramirez-Sanchez,Carmen Clapp,Alexandra Luna-Angulo,Alvaro Rendon,Elva Adan-Castro,Gabriela Ramírez-Hernández,Nundehui Díaz-Lezama,Ramón M Coral-Vázquez,Francisco J Estrada-Mena

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Melanoma Inhibitory Activity antibody [EPR15597]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma Inhibitory Activity antibody [EPR15597] (AB186731)

Western blot - Anti-Melanoma Inhibitory Activity antibody [EPR15597] (AB186731)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

Product promise