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AB2716

Anti-Melanophilin antibody

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(1 Publication)

Goat Polyclonal MELPH antibody. Suitable for WB, ICC/IF and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Mouse Mlph aa 550 to C-terminus.

View Alternative Names

SLAC2A, MLPH, Melanophilin, Exophilin-3, Slp homolog lacking C2 domains a, Synaptotagmin-like protein 2a, SlaC2-a

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Melanophilin antibody (AB2716)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Melanophilin antibody (AB2716)

Wild-type C57bl6 derived melanocytes were stained with ab2716 (0.5ug/ml) and detected with Alexa568 conjugated donkey anti-sheep second antibodies. Brightfield image shows melanosome distribution. Data kindly provided by A N Hume, Imperial College.

Western blot - Anti-Melanophilin antibody (AB2716)
  • WB

Unknown

Western blot - Anti-Melanophilin antibody (AB2716)

ab2716 staining (0.5ug/ml) of total melanocyte PNS (200ug per lane) of wild-type and leaden (Mlph null) mice. The upper band may represent the stacking-separating gel interface.

All lanes:

Western blot - Anti-Melanophilin antibody (ab2716) at 0.5 µg/mL

Lane 1:

total melanocyte PNS (200ug per lane) of wild-type

Lane 2:

total melanocyte PNS (200ug per lane) of leaden (Mlph null) mice

Predicted band size: 66 kDa

Observed band size: 81 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Mouse Mlph aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q91V27

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Complementary Products

Primary Antibodies

AB235003

Anti-MYO5A antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYO5A antibody (AB235003)

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Primary Antibodies

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Anti-Calnexin antibody - ER Marker

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Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

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Primary Antibodies

AB180753

Anti-Tyrosinase antibody

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Rab effector protein involved in melanosome transport. Serves as link between melanosome-bound RAB27A and the motor protein MYO5A.
See full target information MLPH
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 12:e0171513 PubMed28182699

2017

Bifunctional effects of O-methylated flavones from Scutellaria baicalensis Georgi on melanocytes: Inhibition of melanin production and intracellular melanosome transport.

Applications

Unspecified application

Species

Unspecified reactive species

Michiko Kudo,Kumiko Kobayashi-Nakamura,Kentaro Tsuji-Naito
View all publications
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Product promise

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