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AB327588

Anti-Melatonin Receptor 1A antibody [HL4732] - BSA and Azide free

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Rabbit Recombinant Monoclonal Melatonin Receptor 1A/MTNR1A antibody. Carrier free. Suitable for WB and reacts with Transfected cell lysate - Human, Mouse, Rat samples.
3 Images
Western blot - Anti-Melatonin Receptor 1A antibody [HL4732] - BSA and Azide free (AB327588)
  • WB

Supplier Data

Western blot - Anti-Melatonin Receptor 1A antibody [HL4732] - BSA and Azide free (AB327588)

Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ab327588 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Melatonin Receptor 1A antibody [HL4732] - BSA and Azide free (ab327588) at 1/5000 dilution

Lane 1:

Non-transfected 293T whole cell extract (unboiled) at 30 µg

Lane 2:

HA-tagged MTNR1A transfected 293T whole cell extract (unboiled) at 30 µg

Lane 3:

DDDDK-tagged MTNR1B transfected 293T whole cell extract (unboiled) at 30 µg

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Western blot - Anti-Melatonin Receptor 1A antibody [HL4732] - BSA and Azide free (AB327588)
  • WB

Supplier Data

Western blot - Anti-Melatonin Receptor 1A antibody [HL4732] - BSA and Azide free (AB327588)

Boiled and unboiled various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ab327588 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Melatonin Receptor 1A antibody [HL4732] - BSA and Azide free (ab327588) at 1/1000 dilution

Lane 1:

Rat eye tissue extract (boiled) at 1/50 dilution

Lane 2:

Rat liver tissue extract (boiled) at 1/50 dilution

Lane 3:

Rat eye tissue extract (unboiled) at 50 µg

Lane 4:

Rat liver tissue extract (unboiled) at 1/50 dilution

false

Western blot - Anti-Melatonin Receptor 1A antibody [HL4732] - BSA and Azide free (AB327588)
  • WB

Supplier Data

Western blot - Anti-Melatonin Receptor 1A antibody [HL4732] - BSA and Azide free (AB327588)

Unboiled various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ab327588 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Melatonin Receptor 1A antibody [HL4732] - BSA and Azide free (ab327588) at 1/1000 dilution

Lane 1:

Mouse testis tissue extract (unboiled) at 50 µg

Lane 2:

Mouse eye tissue extract (unboiled) at 50 µg

Lane 3:

Mouse liver tissue extract (unboiled) at 50 µg

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL4732

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

<p>This antibody is specific for human MTNR1A protein and does not cross react with human MTNR1B protein.</p>

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }
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Product details

It was raised against human Melatonin Receptor 1A Extracellular domain.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

High affinity receptor for melatonin. Likely to mediate the reproductive and circadian actions of melatonin. The activity of this receptor is mediated by pertussis toxin sensitive G proteins that inhibit adenylate cyclase activity. Possibly involved in sleep induction, by melatonin activation of the potassium channel KCNMA1/BK and the dissociation of G-beta and G-gamma subunits, thereby decreasing synaptic transmission (By similarity).
See full target information Melatonin receptor type 1A

Alternative Names

Melatonin receptor type 1A, Mel-1A-R, Mel1a receptor, MTNR1A
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com