Rabbit Polyclonal Melatonin Receptor 1B/MTNR1B antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Rat, Mouse samples. Cited in 39 publications. Immunogen corresponding to Synthetic Peptide within Human MTNR1B aa 200-300 conjugated to Keyhole Limpet Haemocyanin.
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- Neurology and therapy 12:2021-20402023Effect of PLA2G6 and SMPD1 Variants on the Lipid Metabolism in the Cerebrospinal Fluid of Patients with Parkinson's Disease: A Non-targeted Lipidomics Study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYongang Li,GuiKai Ji,Mengjia Lian,Xuan Liu,Ying Xu,Yaxing GuiPubMed 37707705
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e231142023Convergent alteration of the mesenchymal stem cell heterogeneity in adipose tissue during aging.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiwei Liu,Yu Zhao,Qian Wang,Li Yan,Xin Fu,Ran XiaoPubMed 37498236
- Frontiers in pharmacology 14:12103932023Melatonin-related dysfunction in chronic restraint stress triggers sleep disorders in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTian-Ji Xia,Zhi Wang,Su-Wei Jin,Xin-Min Liu,Yong-Guang Liu,Shan-Shan Zhang,Rui-Le Pan,Ning Jiang,Yong-Hong Liao,Ming-Zhu Yan,Li-Da Du,Qi ChangPubMed 37408758
- International journal of molecular sciences 24:2023The Uterine Melatonergic Systems of AANAT and Melatonin Membrane Receptor 2 (MT2) Are Essential for Endometrial Receptivity and Early Implantation in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao Ma,Jing Wang,Likai Wang,Laiqing Yan,Yunjie Liu,Wenkui Ma,Pengyun Ji,Lu Zhang,Guoshi LiuPubMed 37108290
- Antioxidants (Basel, Switzerland) 12:2023Melatonin Supplementation during the Late Gestational Stage Enhances Reproductive Performance of Sows by Regulating Fluid Shear Stress and Improving Placental Antioxidant Capacity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLikai Wang,Laiqing Yan,Qi Han,Guangdong Li,Hao Wu,Xiao Ma,Mengmeng Zhao,Wenkui Ma,Pengyun Ji,Ran Zhang,Guoshi LiuPubMed 36978937
- Journal of pineal research 74:e128512023Melatonin increases susceptibility to atrial fibrillation in obesity via Akt signaling impairment in response to lipid overload.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinghua Qin,Yuping Fu,Jiali Fan,Binghua Liu,Peng Liu,Yudi Zhang,Tiannan Jiang,Qiangsun ZhengPubMed 36639364
- International journal of molecular sciences 23:2022Melatonin Type 2 Receptor Activation Regulates Blue Light Exposure-Induced Mouse Corneal Epithelial Damage by Modulating Impaired Autophagy and Apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRujun Jin,Ying Li,Hui Jin,Hee Su Yoon,Ji Suk Choi,Jonghwa Kim,Hyeon Jeong Yoon,Kyung Chul YoonPubMed 36232639
- Frontiers in pharmacology 13:9258282022Shumian Capsule Improves the Sleep Disorder and Mental Symptoms Through Melatonin Receptors in Sleep-Deprived Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenhua Li,Yinlong Cheng,Yi Zhang,Yazhi Qian,Mo Wu,Wei Huang,Nan Yang,Yanyong LiuPubMed 35873551
- Cells 11:2022Activation of MT1/MT2 to Protect Testes and Leydig Cells against Cisplatin-Induced Oxidative Stress through the SIRT1/Nrf2 Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunqiang Zhang,Yuan Fang,Dongdong Tang,Xingyu Xu,Xiaoqian Zhu,Shusheng Wu,Hui Yu,Huiru Cheng,Ting Luo,Qunshan Shen,Yang Gao,Cong Ma,Yajing Liu,Zhaolian Wei,Xiaoyu Chen,Fangbiao Tao,Xiaojin He,Yunxia CaoPubMed 35626727
- PeerJ 10:e134612022Melatonin increases growth properties in human dermal papilla spheroids by activating AKT/GSK3β/β-Catenin signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSowon Bae,Yoo Gyeong Yoon,Ji Yea Kim,In-Chul Park,Sungkwan An,Jae Ho Lee,Seunghee BaePubMed 35607451
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human MTNR1B aa 200-300 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P49286
Consider this alternative
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100.00000 - 1/500.00000 | Notes When using a fluorescent probe please use a dilution of 1/50 - 1/200. |
Species Mouse | Dilution info 1/100.00000 - 1/500.00000 | Notes When using a fluorescent probe please use a dilution of 1/50 - 1/200. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
High affinity receptor for melatonin. Likely to mediate the reproductive and circadian actions of melatonin. The activity of this receptor is mediated by pertussis toxin sensitive G proteins that inhibit adenylate cyclase activity.
Alternative names
Melatonin receptor type 1B, Mel-1B-R, Mel1b receptor, MTNR1B
Recommended products
Rabbit Polyclonal Melatonin Receptor 1B/MTNR1B antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Rat, Mouse samples. Cited in 39 publications. Immunogen corresponding to Synthetic Peptide within Human MTNR1B aa 200-300 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human MTNR1B aa 200-300 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P49286
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melatonin Receptor 1B/MTNR1B antibody (ab203346)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain sections labeling Melatonin Receptor 1B/MTNR1B with ab203346 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melatonin Receptor 1B/MTNR1B antibody (ab203346)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat retina sections labeling Melatonin Receptor 1B/MTNR1B with ab203346 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melatonin Receptor 1B/MTNR1B antibody (ab203346)
Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum section; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ab203346 at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Immunocytochemistry/ Immunofluorescence - Anti-Melatonin Receptor 1B/MTNR1B antibody (ab203346)
ab203346 staining MTNR1B in HeLa cells. The cells were fixed with 4% Paraformaldehyde, permeabilized with Triton X-100 at room temperature for 20 min; blocked with normal goat serum at 37°C for 20 min then incubated with ab203346 at 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Western blot - Anti-Melatonin Receptor 1B/MTNR1B antibody (ab203346)
HepG2 cell lysates probed with ab203346 at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
All lanes: Western blot - Anti-Melatonin Receptor 1B/MTNR1B antibody (ab203346) at 1/1000 dilution
All lanes: Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Predicted band size: 40 kDa
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