Anti-Melatonin Receptor 1B/MTNR1B antibody

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Melatonin Receptor 1B/MTNR1B antibody (AB203346)

ab203346 staining MTNR1B in HeLa cells. The cells were fixed with 4% Paraformaldehyde, permeabilized with Triton X-100 at room temperature for 20 min; blocked with normal goat serum at 37°C for 20 min then incubated with ab203346 at 1 : 100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melatonin Receptor 1B/MTNR1B antibody (AB203346)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat retina sections labeling Melatonin Receptor 1B/MTNR1B with ab203346 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melatonin Receptor 1B/MTNR1B antibody (AB203346)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain sections labeling Melatonin Receptor 1B/MTNR1B with ab203346 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melatonin Receptor 1B/MTNR1B antibody (AB203346)

Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum section; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ab203346 at 1 : 200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

• WB

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Western blot - Anti-Melatonin Receptor 1B/MTNR1B antibody (AB203346)

HepG2 cell lysates probed with ab203346 at 1 : 1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1 : 20000 for 60 min at 37˚C.

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Western blot - Anti-Melatonin Receptor 1B/MTNR1B antibody (ab203346) at 1/1000 dilution

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Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

Predicted band size: 40 kDa

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