Anti-Melatonin Receptor 1B/MTNR1B antibody [EPR28274-48]

• WB

Lab

Western blot - Anti-Melatonin Receptor 1B/MTNR1B antibody [EPR28274-48] (AB325243)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : liver, lung (PMID : 19060909), tonsil.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-Melatonin Receptor 1B/MTNR1B antibody [EPR28274-48] (ab325243) at 1/1000 dilution

Lane 1:

Human alzheimer's brain tissue lysate at 4 µg

Lane 2:

Human striatum tissue lysate at 4 µg

Lane 3:

Human cerebellum tissue lysate at 4 µg

Lane 4:

Human thalamus tissue lysate at 4 µg

Lane 5:

Human liver tissue lysate at 4 µg

Lane 6:

Human tonsil tissue lysate at 4 µg

Lane 7:

Human lung tissue lysate at 4 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 37-48 kDa,36 kDa

false

Exposure time: 26s

• Dot

Lab

Dot Blot - Anti-Melatonin Receptor 1B/MTNR1B antibody [EPR28274-48] (AB325243)

Dot blot analysis of Melatonin Receptor 1B/MTNR1B using ab325243 at 1 : 1000 (0.512 μg/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane 1 : 293T (human embryonic kidney epithelial cell) transfected with MTNR1B expression vector containing a myc-His-tag® whole cell lysate

Lane 2 : 293T (human embryonic kidney epithelial cell) transfected with MTNR1A expression vector containing a myc-His-tag® whole cell lysate

Exposure time : 180 seconds..

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with overexpressed human MTNR1A by dot blot.

All lanes:

Dot Blot - Anti-Melatonin Receptor 1B/MTNR1B antibody [EPR28274-48] (ab325243) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with MTNR1B expression vector containing a myc-His-tag® whole cell lysate

Lane 2:

293T (human embryonic kidney epithelial cell) transfected with MTNR1A expression vector containing a myc-His-tag® whole cell lysate

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-Melatonin Receptor 1B/MTNR1B antibody [EPR28274-48] (AB325243)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : liver, lung (PMID : 19060909).

The identity of the bands higher than 50 kDa and lower than 37 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-Melatonin Receptor 1B/MTNR1B antibody [EPR28274-48] (ab325243) at 1/1000 dilution

Lane 1:

Mouse cerebellum tissue lysate at 30 µg

Lane 2:

Mouse brain tissue lysate at 30 µg

Lane 3:

Mouse hippocampus tissue lysate at 30 µg

Lane 4:

Mouse liver tissue lysate at 30 µg

Lane 5:

Mouse lung tissue lysate at 30 µg

Lane 6:

Rat brain tissue lysate at 30 µg

Lane 7:

Rat hippocampus tissue lysate at 30 µg

Lane 8:

Rat liver tissue lysate at 30 µg

Lane 9:

Rat lung tissue lysate at 30 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 37-48 kDa,36 kDa

false

Exposure time: 26s