AB129373

Anti-MELK antibody [2G2]

Name: Anti-MELK antibody [2G2] (ab129373) | Abcam
Brand: Abcam Limited
SKU: AB129373
Price: 545 USD
Availability: InStock

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(7 Publications)

Mouse Monoclonal MELK antibody. Suitable for Flow Cyt, IHC-P, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human MELK aa 600 to C-terminus.

View Alternative Names

KIAA0175, MELK, Maternal embryonic leucine zipper kinase, hMELK, Protein kinase Eg3, Protein kinase PK38, Tyrosine-protein kinase MELK, pEg3 kinase, hPK38

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MELK antibody [2G2] (AB129373)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MELK antibody [2G2] (AB129373)

ab129373 at 1/200 dilution staining MELK in paraffin-embedded liver cancer tissue by immunohistochemistry, with DAB staining.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MELK antibody [2G2] (AB129373)

ab129373 at 1/200 dilution staining MELK in paraffin-embedded liver cancer tissue by immunohistochemistry, with DAB staining.

Immunocytochemistry/ Immunofluorescence - Anti-MELK antibody [2G2] (AB129373)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MELK antibody [2G2] (AB129373)

ab129373 at 1/200 dilution staining MELK (green) in HepG2 cells by immunofluorescence. DNA is stained with DRAQ fluorescent dye (blue) and actin filaments with Alexa Fluor-555 phalloidin (red).

Flow Cytometry - Anti-MELK antibody [2G2] (AB129373)

Flow Cyt

Unknown

Flow Cytometry - Anti-MELK antibody [2G2] (AB129373)

ab129373 at 1/200 dilution (green) or negative control (red) staining MELK in MCF-7 cells by flow cytometry.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2G2

Isotype

IgG2a

Carrier free

Reacts with

Human

Applications

IHC-P, Flow Cyt, ICC/IF

applications

Immunogen

Synthetic Peptide within Human MELK aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q14680

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus tropicalis": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

This product was changed from ascites to supernatant. Lot no's high than GR248807-19 are from Tissue Culture Supernatant

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Maternal embryonic leucine zipper kinase commonly referred to as MELK is a serine/threonine protein kinase with a molecular mass of about 74 kDa. MELK is broadly expressed in both human and animal tissues with significant presence in embryonic and adult stem cells. Researchers often recognize it as MELK or MELK-0A21 in studies. The protein is characterized by its regulation of cell division and apoptosis making it an important player in cellular proliferation.

Biological function summary

MELK regulates signals that control the cell cycle apoptosis and embryonic development. MELK works as a component of complexes involved in maintaining cellular homeostasis. The protruding roles involve influencing cellular architecture and governance of stem cell maintenance. Studies in animal models show MELK involvement in maintaining pluripotency and regulation during the development phase.

Pathways

MELK participates in key processes such as the cell cycle and apoptosis pathways. Its diverse functionality connects MELK to proteins like Bcl-2-associated death promoter (BAD) and p53 which further influence main cellular events. These pathways and interactions make MELK a target of interest in the study of cellular growth and programmed cell death.

Researchers link MELK to cancer and neurodegenerative diseases. The overexpression of MELK is frequently observed in various cancer types making it relevant to oncogenesis. Studies examine its relation to proteins like cyclin-dependent kinases (CDKs) and survivin in cancer progression. Additionally MELK-0A21 variants may offer insights in disorders like Glioblastoma where abnormal MELK activity aligns with disease aggression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.

See full target information MELK

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:109827 PubMed38827395

2024

DDX56 promotes EMT and cancer stemness via MELK-FOXM1 axis in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Li,Tianyi Wang,Ximin Wang,XinYu Ge,Tao Yang,Wei Wang

Journal of inflammation (London, England) 20:35 PubMed37915070

2023

The TFPI2-PPARγ axis induces M2 polarization and inhibits fibroblast activation to promote recovery from post-myocardial infarction in diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Mengqi Guo,Zongyi Xia,Yefeng Hong,Hongwei Ji,Fuhai Li,Wenheng Liu,Shaohua Li,Hui Xin,Kai Tan,Zhexun Lian

Frontiers in neurology 13:977180 PubMed36353126

2022

MELK is a prognostic biomarker and correlated with immune infiltration in glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Yang,Huandi Zhou,Guohui Wang,Lei Tian,Haonan Li,Yufeng Zhang,Xiaoying Xue

Oncology letters 23:144 PubMed35340554

2022

Identification of potential circular RNA biomarkers in lung adenocarcinoma: A bioinformatics analysis and retrospective clinical study.

Applications

Unspecified application

Species

Unspecified reactive species

Yonggang Zhu,Feng Cao,Fei Liu,Sihua Liu,Lingjiao Meng,Lina Gu,Hanjun Zhao,Meixiang Sang,Baoen Shan

Digestive surgery 39:24-31 PubMed34788753

2021

Identification and Validation of Hub Genes Predicting Prognosis of Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ziyan Chen,Haitao Yu,Lijun Wu,Sina Zhang,Zhihui Lin,Tuo Deng,Bangjie He,Zhengping Yu,Gang Chen,Fang Wu

Oncology letters 20:147 PubMed32934715

2020

Maternal embryonic leucine zipper kinase: A novel biomarker and a potential therapeutic target in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shengsong Chen,Zhanpeng Lu,Xiaoyong Chen,Xiya Wu,Hongying Tu,Lingling Yu,Zuke Xiao

Neuro-oncology 22:58-69 PubMed31504799

2019

MEK/MELK inhibition and blood-brain barrier deficiencies in atypical teratoid/rhabdoid tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Michaël H Meel,Miriam Guillén Navarro,Mark C de Gooijer,Dennis S Metselaar,Piotr Waranecki,Marjolein Breur,Tonny Lagerweij,Laurine E Wedekind,Jan Koster,Marianne D van de Wetering,Netteke Schouten-van Meeteren,Eleonora Aronica,Olaf van Tellingen,Marianna Bugiani,Timothy N Phoenix,Gertjan J L Kaspers,Esther Hulleman

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-MELK antibody [2G2]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MELK antibody [2G2] (AB129373)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MELK antibody [2G2] (AB129373)

Immunocytochemistry/ Immunofluorescence - Anti-MELK antibody [2G2] (AB129373)

Flow Cytometry - Anti-MELK antibody [2G2] (AB129373)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise