Mouse Monoclonal MELK antibody. Suitable for Flow Cyt, IHC-P, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human MELK aa 600 to C-terminus.
Images
![Flow Cytometry - Anti-MELK antibody [2G2] (AB129373), expandable thumbnail](https://content.abcam.com/products/images/melk-antibody-2g2-ab129373--flow-cytometry-img128573.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-MELK antibody [2G2] (AB129373)")
![Immunocytochemistry/ Immunofluorescence - Anti-MELK antibody [2G2] (AB129373), expandable thumbnail](https://content.abcam.com/products/images/melk-antibody-2g2-ab129373--immunocytochemistry-immunofluorescence-img84448.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-MELK antibody [2G2] (AB129373)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MELK antibody [2G2] (AB129373), expandable thumbnail](https://content.abcam.com/products/images/melk-antibody-2g2-ab129373--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img74740.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MELK antibody [2G2] (AB129373)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MELK antibody [2G2] (AB129373), expandable thumbnail](https://content.abcam.com/products/images/melk-antibody-2g2-ab129373--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img103071.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MELK antibody [2G2] (AB129373)")
Publications
Filter by
- Frontiers in neurology 13:9771802022MELK is a prognostic biomarker and correlated with immune infiltration in glioma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaiyan Yang,Huandi Zhou,Guohui Wang,Lei Tian,Haonan Li,Yufeng Zhang,Xiaoying XuePubMed 36353126
- Oncology letters 23:1442022Identification of potential circular RNA biomarkers in lung adenocarcinoma: A bioinformatics analysis and retrospective clinical study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYonggang Zhu,Feng Cao,Fei Liu,Sihua Liu,Lingjiao Meng,Lina Gu,Hanjun Zhao,Meixiang Sang,Baoen ShanPubMed 35340554
- Digestive surgery 39:24-312021Identification and Validation of Hub Genes Predicting Prognosis of Hepatocellular Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZiyan Chen,Haitao Yu,Lijun Wu,Sina Zhang,Zhihui Lin,Tuo Deng,Bangjie He,Zhengping Yu,Gang Chen,Fang WuPubMed 34788753
- Oncology letters 20:1472020Maternal embryonic leucine zipper kinase: A novel biomarker and a potential therapeutic target in lung adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShengsong Chen et. al.PubMed 32934715
- Neuro-oncology 22:58-692019MEK/MELK inhibition and blood-brain barrier deficiencies in atypical teratoid/rhabdoid tumors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichaël H Meel,Miriam Guillén Navarro,Mark C de Gooijer,Dennis S Metselaar,Piotr Waranecki,Marjolein Breur,Tonny Lagerweij,Laurine E Wedekind,Jan Koster,Marianne D van de Wetering,Netteke Schouten-van Meeteren,Eleonora Aronica,Olaf van Tellingen,Marianna Bugiani,Timothy N Phoenix,Gertjan J L Kaspers,Esther HullemanPubMed 31504799
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human MELK aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q14680
Reactivity data
Select an application| Flow Cyt | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted | Predicted |
| Xenopus tropicalis | Predicted | Predicted | Predicted |
| Zebrafish | Predicted | Predicted | Predicted |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/400.00000 | Notes ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Xenopus laevis, Zebrafish, Xenopus tropicalis | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Xenopus laevis, Zebrafish, Xenopus tropicalis | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Xenopus laevis, Zebrafish, Xenopus tropicalis | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.
Alternative names
KIAA0175, MELK, Maternal embryonic leucine zipper kinase, hMELK, Protein kinase Eg3, Protein kinase PK38, Tyrosine-protein kinase MELK, pEg3 kinase, hPK38
Recommended products
Mouse Monoclonal MELK antibody. Suitable for Flow Cyt, IHC-P, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human MELK aa 600 to C-terminus.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Human MELK aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q14680
- Clone number
- 2G2
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purified from tissue culture supernatant.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
Notes
This product was changed from ascites to supernatant. Lot no's high than GR248807-19 are from Tissue Culture Supernatant
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Maternal embryonic leucine zipper kinase commonly referred to as MELK is a serine/threonine protein kinase with a molecular mass of about 74 kDa. MELK is broadly expressed in both human and animal tissues with significant presence in embryonic and adult stem cells. Researchers often recognize it as MELK or MELK-0A21 in studies. The protein is characterized by its regulation of cell division and apoptosis making it an important player in cellular proliferation.
- Biological function summary
MELK regulates signals that control the cell cycle apoptosis and embryonic development. MELK works as a component of complexes involved in maintaining cellular homeostasis. The protruding roles involve influencing cellular architecture and governance of stem cell maintenance. Studies in animal models show MELK involvement in maintaining pluripotency and regulation during the development phase.
- Pathways
MELK participates in key processes such as the cell cycle and apoptosis pathways. Its diverse functionality connects MELK to proteins like Bcl-2-associated death promoter (BAD) and p53 which further influence main cellular events. These pathways and interactions make MELK a target of interest in the study of cellular growth and programmed cell death.
- Associated diseases and disorders
Researchers link MELK to cancer and neurodegenerative diseases. The overexpression of MELK is frequently observed in various cancer types making it relevant to oncogenesis. Studies examine its relation to proteins like cyclin-dependent kinases (CDKs) and survivin in cancer progression. Additionally MELK-0A21 variants may offer insights in disorders like Glioblastoma where abnormal MELK activity aligns with disease aggression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Flow Cytometry - Anti-MELK antibody [2G2] (ab129373), expandable thumbnail](https://content.abcam.com/products/images/melk-antibody-2g2-ab129373--flow-cytometry-img128573.jpg "Flow Cytometry - Anti-MELK antibody [2G2] (ab129373)")
Flow Cytometry - Anti-MELK antibody [2G2] (ab129373)
ab129373 at 1/200 dilution (green) or negative control (red) staining MELK in MCF-7 cells by flow cytometry.
![Immunocytochemistry/ Immunofluorescence - Anti-MELK antibody [2G2] (ab129373), expandable thumbnail](https://content.abcam.com/products/images/melk-antibody-2g2-ab129373--immunocytochemistry-immunofluorescence-img84448.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MELK antibody [2G2] (ab129373)")
Immunocytochemistry/ Immunofluorescence - Anti-MELK antibody [2G2] (ab129373)
ab129373 at 1/200 dilution staining MELK (green) in HepG2 cells by immunofluorescence. DNA is stained with DRAQ fluorescent dye (blue) and actin filaments with Alexa Fluor-555 phalloidin (red).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MELK antibody [2G2] (ab129373), expandable thumbnail](https://content.abcam.com/products/images/melk-antibody-2g2-ab129373--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img74740.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MELK antibody [2G2] (ab129373)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MELK antibody [2G2] (ab129373)
ab129373 at 1/200 dilution staining MELK in paraffin-embedded liver cancer tissue by immunohistochemistry, with DAB staining.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MELK antibody [2G2] (ab129373), expandable thumbnail](https://content.abcam.com/products/images/melk-antibody-2g2-ab129373--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img103071.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MELK antibody [2G2] (ab129373)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MELK antibody [2G2] (ab129373)
ab129373 at 1/200 dilution staining MELK in paraffin-embedded liver cancer tissue by immunohistochemistry, with DAB staining.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com