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AB97554

Anti-Melphalan antibody [Amp4/42]

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(5 Publications)

Mouse Monoclonal Melphalan antibody Amp4/42. Suitable for ELISA and reacts with Chemical samples. Cited in 5 publications.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Amp4/42

Isotype

IgG2b

Carrier free

No

Applications

ELISA

applications

Specificity

This antibody recognises the ring-opened structure that forms when DNA is alkylated by the anti-cancer drug melphalan and is then exposed to alkali. The alkali causes guanine bases alkylated at the N7 position to undergo a so-called ring-opening reaction and this antibody appears to recognise this structure, although recognition may be influenced by the neighbouring DNA sequence. The products of the ring-opening reaction are more resistant to depurination reactions than the original guanine adducts. This antibody was developed to improve detection of melphalan-DNA adducts through its ability to recognise the stabilised adduct. The Amp4/42 clone did not bind to alkali-treated control DNA or to DNA that had been alkylated with melphalan but not exposed to alkali. This antibody can be used for quantification of melphalan adducts in purified DNA by ELISA and for immunofluorescent detection of melphalan-DNA adducts in single cells.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Melphalan
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Biochemical pharmacology 67:1771-8 PubMed15081876

2004

Immunohistochemical detection of melphalan-DNA adducts in colon cancer cells in vitro and human colorectal liver tumours in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Joost Rothbarth,Cindy Koevoets,Rob A E M Tollenaar,Mike J Tilby,Cornelis J H van de Velde,Gerard J Mulder,Peter J K Kuppen

Experimental cell research 283:127-34 PubMed12581733

2003

Quantification of DNA adducts in individual cells by immunofluorescence: effects of variation in DNA conformation.

Applications

Unspecified application

Species

Unspecified reactive species

Adrian J Frank,Michael J Tilby

Blood 100:845-53 PubMed12130494

2002

Down-regulation of DNA repair in human CD34(+) progenitor cells corresponds to increased drug sensitivity and apoptotic response.

Applications

Unspecified application

Species

Unspecified reactive species

Claudia Buschfort-Papewalis,Thomas Moritz,Bernd Liedert,Jürgen Thomale

Chemical research in toxicology 14:71-81 PubMed11170510

2001

Antibody recognition of melphalan adducts characterized using immobilized DNA: enhanced alkylation of G-Rich regions in cells compared to in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

H McCartney,A M Martin,P G Middleton,M J Tilby

Carcinogenesis 16:1895-901 PubMed7634420

1995

A monoclonal antibody that recognizes alkali-stabilized melphalan-DNA adducts and its application in immunofluorescence microscopy.

Applications

Unspecified application

Species

Unspecified reactive species

M J Tilby,H McCartney,J Cordell,A J Frank,C J Dean
View all publications
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