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Rabbit Recombinant Monoclonal Menin antibody. Suitable for IHC-P, ICC/IF, IP, WB and reacts with Mouse, Rat, Human samples. Cited in 12 publications.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody [EPR3986] (AB92443), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody [EPR3986] (AB92443), expandable thumbnail
  • Immunoprecipitation - Anti-Menin antibody [EPR3986] (AB92443), expandable thumbnail
  • Immunoprecipitation - Anti-Menin antibody [EPR3986] (AB92443), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody [EPR3986] (AB92443), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFIPFlow CytWB
Human
Tested
Tested
Tested
Not recommended
Tested
Mouse
Tested
Expected
Tested
Not recommended
Tested
Rat
Tested
Expected
Expected
Not recommended
Tested

Tested
Tested

Species
Mouse
Dilution info
1/100 - 1/500
Notes

For unpurified use between 1/100-1/250.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/100 - 1/500
Notes

For unpurified use between 1/100-1/250.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/100 - 1/500
Notes

For unpurified use between 1/100-1/250.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/50
Notes

For unpurified use between 1/250-1/500.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/10 - 1/100
Notes

-

Species
Human
Dilution info
1/10 - 1/100
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

For unpurified use between 1/10,000-1/50,000.

Species
Rat
Dilution info
1/1000
Notes

For unpurified use between 1/10,000-1/50,000.

Species
Human
Dilution info
1/1000
Notes

For unpurified use between 1/10,000-1/50,000.

Associated Products

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Target data

Function

Essential component of a MLL/SET1 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3 (H3K4). Functions as a transcriptional regulator. Binds to the TERT promoter and represses telomerase expression. Plays a role in TGFB1-mediated inhibition of cell-proliferation, possibly regulating SMAD3 transcriptional activity. Represses JUND-mediated transcriptional activation on AP1 sites, as well as that mediated by NFKB subunit RELA. Positively regulates HOXC8 and HOXC6 gene expression. May be involved in normal hematopoiesis through the activation of HOXA9 expression (By similarity). May be involved in DNA repair.

Alternative names

SCG2, MEN1, Menin

Recommended products

Rabbit Recombinant Monoclonal Menin antibody. Suitable for IHC-P, ICC/IF, IP, WB and reacts with Mouse, Rat, Human samples. Cited in 12 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR3986
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody [EPR3986] (ab92443), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody [EPR3986] (ab92443)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labelling Menin with purified ab92443 at 1/100 dilution (6.08 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody [EPR3986] (ab92443), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody [EPR3986] (ab92443)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labelling Menin with purified ab92443 at 1/100 dilution (6.08 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunoprecipitation - Anti-Menin antibody [EPR3986] (ab92443), expandable thumbnail

    Immunoprecipitation - Anti-Menin antibody [EPR3986] (ab92443)

    Purified ab92443 at 1/50 dilution (2μg) immunoprecipitating Menin in Jurkat whole cell lysate.
    Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10μg
    Lane 2 (+): ab92443 + Jurkat whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab92443 in Jurkat whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 75 kDa

    All lanes: Immunoprecipitation - Anti-Menin antibody [EPR3986] (ab92443)

    Predicted band size: 68 kDa

  • Immunoprecipitation - Anti-Menin antibody [EPR3986] (ab92443), expandable thumbnail

    Immunoprecipitation - Anti-Menin antibody [EPR3986] (ab92443)

    Menin was immunoprecipitated from 0.35 mg MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate 10 ug with ab92443 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab92443 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate 10 μg

    Lane 2: ab92443 IP in MEF whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab92443 in MEF whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes

    All lanes: Immunoprecipitation - Anti-Menin antibody [EPR3986] (ab92443)

    Predicted band size: 68 kDa

    Observed band size: 75 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody [EPR3986] (ab92443), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody [EPR3986] (ab92443)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue sections labelling Menin with purified ab92443 at 1/500 dilution (1.22 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Western blot - Anti-Menin antibody [EPR3986] (ab92443), expandable thumbnail

    Western blot - Anti-Menin antibody [EPR3986] (ab92443)

    Lane 1: Wild-type HAP1 cell lysate (20 μg)
    Lane 2: Menin knockout HAP1 cell lysate (20 μg)
    Lane 3: Jurkat cell lysate (20 μg)
    Lane 4: A431 cell lysate (20 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab92443 observed at 74 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    ab92443 was shown to recognize Menin when Menin knockout samples were used, along with additional cross-reactive bands. Wild-type and Menin knockout samples were subjected to SDS-PAGE. ab92443 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/10,000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-Menin antibody [EPR3986] (ab92443)

    Predicted band size: 68 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Menin antibody [EPR3986] (ab92443), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Menin antibody [EPR3986] (ab92443)

    Immunocytochemistry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling Menin with purified ab92443 at 1/50 dilution (12.1 μg/mL). Cells were fixed in 100% Methanol and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.6 μg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) ab150078) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-Menin antibody [EPR3986] (ab92443), expandable thumbnail

    Western blot - Anti-Menin antibody [EPR3986] (ab92443)

    All lanes: Western blot - Anti-Menin antibody [EPR3986] (ab92443) at 1/1000 dilution

    Lane 1: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 15 µg

    Lane 2: MEF (Mouse embryonic fibroblast (immortalized)) whole cell lysate at 15 µg

    Lane 3: PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 68 kDa

    Observed band size: 75 kDa

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Product protocols

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