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AB185991

Anti-MEPCE antibody - C-terminal

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(2 Publications)

Rabbit Polyclonal MEPCE antibody. C-terminal. Suitable for IP, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human MEPCE aa 600 to C-terminus.

View Alternative Names

BCDIN3, MEPCE, 7SK snRNA methylphosphate capping enzyme, MePCE, Bicoid-interacting protein 3 homolog, Bin3 homolog

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEPCE antibody - C-terminal (AB185991)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEPCE antibody - C-terminal (AB185991)

Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human ovarian carcinoma tissue labeling MEPCE with ab185991 at a dilution of 1/1000.

Immunoprecipitation - Anti-MEPCE antibody - C-terminal (AB185991)
  • IP

Supplier Data

Immunoprecipitation - Anti-MEPCE antibody - C-terminal (AB185991)

Detection of MEPCE in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab185991 at 6 μg/mg lysate for IP (Lane 1). For WB detection an anti-MEPCE antibody was used at 1μg/ml. Lane 2 represents control IgG IP. Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-MEPCE antibody - C-terminal (ab185991)

Predicted band size: 74 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, IHC-P

applications

Immunogen

Synthetic Peptide within Human MEPCE aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q7L2J0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chinese hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab185991 was affinity purified using an epitope specific to MEPCE immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MEPCE protein also known as 3-methylcytosine-specific methyltransferase plays an important mechanical role in RNA processing. MEPCE has a mass of approximately 81 kDa. It adds a methyl group to the 7SK snRNA a critical step in the regulation of transcription. This protein is expressed across various tissues indicating its important functions in different cellular environments.
Biological function summary

The MEPCE protein is an important player in regulating gene expression by stabilizing the 7SK snRNA in a specific ribonucleoprotein complex. This complex controls the activity of the positive transcription elongation factor b (P-TEFb) an important factor in the transcription elongation stage of gene expression. By methylating 7SK snRNA it helps maintain the repression of P-TEFb balancing transcriptional activities.

Pathways

MEPCE works collaboratively within the RNA polymerase II transcription machinery. It participates in the regulation of transcription elongation an important pathway in gene expression. MEPCE interacts closely with other proteins in this process such as LARP7 and HEXIM1 which also associate with the 7SK snRNA to modulate P-TEFb activity.

MEPCE's role in transcription regulation links it to various regulatory diseases including certain cancers and cardiac hypertrophy. The imbalance in P-TEFb activity which MEPCE helps control can lead to unregulated cell proliferation or heart muscle enlargement. MEPCE in association with LARP7 and HEXIM1 adds a layer of complexity to these conditions as alterations in this regulatory system can provoke disease onset or progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA (7SK RNA), leading to stabilize it (PubMed : 17643375, PubMed : 19906723, PubMed : 30559425). Also has a non-enzymatic function as part of the 7SK RNP complex : the 7SK RNP complex sequesters the positive transcription elongation factor b (P-TEFb) in a large inactive 7SK RNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation (PubMed : 17643375). The 7SK RNP complex also promotes snRNA gene transcription by RNA polymerase II via interaction with the little elongation complex (LEC) (PubMed : 28254838). In the 7SK RNP complex, MEPCE is required to stabilize 7SK RNA and facilitate the assembly of 7SK RNP complex (PubMed : 19906723, PubMed : 38100593). MEPCE has a non-enzymatic function in the 7SK RNP complex; interaction with LARP7 within the 7SK RNP complex occluding its catalytic center (PubMed : 19906723). Also required for stability of U6 snRNAs (PubMed : 38100593).
See full target information MEPCE
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 44:115740 PubMed40411785

2025

MePCE promotes homologous recombination through coordinating R-loop resolution at DNA double-stranded breaks.

Applications

Unspecified application

Species

Unspecified reactive species

Sravan K Devanathan,Yi-Ru Li,Samantha B Shelton,Joshuah Nguyen,Wei-Che Tseng,Nakul M Shah,Marvin Mercado,Kyle M Miller,Blerta Xhemalçe

The EMBO journal 36:934-948 PubMed28254838

2017

The 7SK snRNP associates with the little elongation complex to promote snRNA gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Sylvain Egloff,Patrice Vitali,Michael Tellier,Raoul Raffel,Shona Murphy,Tamás Kiss
View all publications
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