Human Recombinant Monoclonal NCAP antibody. Carrier free. Suitable for WB, ICC/IF, I-ELISA and reacts with Recombinant fragment - MERS-CoV, Transfected cell line - MERS-CoV samples.
Images
![Western blot - Anti-MERS-CoV Nucleocapsid protein antibody [abd208] - BSA and Azide free (AB316331), expandable thumbnail](https://content.abcam.com/products/images/mers-cov-nucleocapsid-protein-antibody-abd208-bsa-and-azide-free-ab316331--western-blot-img428910.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MERS-CoV Nucleocapsid protein antibody [abd208] - BSA and Azide free (AB316331)")
![Immunocytochemistry/ Immunofluorescence - Anti-MERS-CoV Nucleocapsid protein antibody [abd208] - BSA and Azide free (AB316331), expandable thumbnail](https://content.abcam.com/products/images/mers-cov-nucleocapsid-protein-antibody-abd208-bsa-and-azide-free-ab316331--immunocytochemistry-immunofluorescence-img428924.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-MERS-CoV Nucleocapsid protein antibody [abd208] - BSA and Azide free (AB316331)")
![Indirect ELISA - Anti-MERS-CoV Nucleocapsid protein antibody [abd208] - BSA and Azide free (AB316331), expandable thumbnail](https://content.abcam.com/products/images/mers-cov-nucleocapsid-protein-antibody-abd208-bsa-and-azide-free-ab316331--indirect-elisa-img428932.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-MERS-CoV Nucleocapsid protein antibody [abd208] - BSA and Azide free (AB316331)")
Key facts
- Isotype
- IgG1
- Host species
- Human
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | I-ELISA | |
|---|---|---|---|
| MERS-CoV | Predicted | Predicted | Predicted |
| Recombinant fragment - MERS-CoV | Tested | Not recommended | Tested |
| Transfected cell line - MERS-CoV | Not recommended | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - MERS-CoV | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species MERS-CoV | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - MERS-CoV | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - MERS-CoV | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species MERS-CoV | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - MERS-CoV | Dilution info - | Notes - |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - MERS-CoV | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species MERS-CoV | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - MERS-CoV | Dilution info - | Notes - |
Human Recombinant Monoclonal NCAP antibody. Carrier free. Suitable for WB, ICC/IF, I-ELISA and reacts with Recombinant fragment - MERS-CoV, Transfected cell line - MERS-CoV samples.
Key facts
- Isotype
- IgG1
- Host species
- Human
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- abd208
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody has been tested only with recombinant MERS-CoV Nucleocapsid protein in western blot.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab316331 is the carrirer-free version of Anti-MERS-CoV Nucleocapsid protein antibody [abd208] ab316330.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The MERS-CoV Nucleocapsid protein also called the N protein plays an important role in the assembly process of the Middle East Respiratory Syndrome Coronavirus (MERS-CoV). The N protein is a structural component that packages the viral RNA genome into a helical ribonucleocapsid which assists in protecting the RNA. This protein which has a mass of approximately 48 kDa is expressed during the viral infection in the host cells particularly in the cytoplasm where it facilitates the encapsulation of the viral genome.
- Biological function summary
The MERS-CoV Nucleocapsid protein interacts closely with other viral proteins including the membrane (M) protein to ensure successful viral replication and assembly. This protein does not work alone; it is part of the larger ribonucleoprotein complex which plays an essential role in stabilizing the viral RNA. Its interaction with the M protein helps in localizing the N protein to specific sites in the host cell preparing the components necessary for virion assembly and release.
- Pathways
The MERS-CoV Nucleocapsid protein engages in processes important for viral pathogenesis and host cell interaction. The protein is part of the coronavirus replication and transcription complex pathway. Within this matrix it associates with non-structural proteins like the RNA-dependent RNA polymerase to enhance RNA synthesis. Additionally it partakes in modulation of host processes to evade the immune response a pivotal aspect of how coronaviruses maintain infection and propagate within the host.
- Associated diseases and disorders
The MERS-CoV Nucleocapsid protein is intimately associated with Middle East Respiratory Syndrome (MERS) a severe respiratory illness. This protein aids the virus in evading the host immune response potentially contributing to disease severity. N protein interactions with host cellular machinery combined with the immune evasion tactics offer insights into viral pathogenicity and might reveal potential therapeutic targets. Understanding these interactions can shed light on how interventions might disrupt the viral life cycle and ameliorate disease impact.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-MERS-CoV Nucleocapsid protein antibody [abd208] - BSA and Azide free (ab316331), expandable thumbnail](https://content.abcam.com/products/images/mers-cov-nucleocapsid-protein-antibody-abd208-bsa-and-azide-free-ab316331--western-blot-img428910.jpg "Western blot - Anti-MERS-CoV Nucleocapsid protein antibody [abd208] - BSA and Azide free (ab316331)")
Western blot - Anti-MERS-CoV Nucleocapsid protein antibody [abd208] - BSA and Azide free (ab316331)
This data was developed using Anti-MERS-CoV Nucleocapsid protein antibody [abd208] ab316330, the same antibody clone in a different buffer formulation.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-MERS-CoV Nucleocapsid protein antibody [abd208] (Anti-MERS-CoV Nucleocapsid protein antibody [abd208] ab316330) at 1/1000 dilution
All lanes: His-tagged MERS-CoV Nucleocapsid recombinant protein at 10 ng with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Human IgG H&L (HRP) (Goat Anti-Human IgG H&L (HRP) ab6858) at 1/5000 dilution
Developed using the ECL technique.
Observed band size: 46 kDa
Exposure time: 180s
![Immunocytochemistry/ Immunofluorescence - Anti-MERS-CoV Nucleocapsid protein antibody [abd208] - BSA and Azide free (ab316331), expandable thumbnail](https://content.abcam.com/products/images/mers-cov-nucleocapsid-protein-antibody-abd208-bsa-and-azide-free-ab316331--immunocytochemistry-immunofluorescence-img428924.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MERS-CoV Nucleocapsid protein antibody [abd208] - BSA and Azide free (ab316331)")
Immunocytochemistry/ Immunofluorescence - Anti-MERS-CoV Nucleocapsid protein antibody [abd208] - BSA and Azide free (ab316331)
This data was developed using Anti-MERS-CoV Nucleocapsid protein antibody [abd208] ab316330, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) transfected with a MERS-CoV Nucleocapsid protein expression vector containing a myc tag. cells labelling MERS-CoV Nucleocapsid protein with Anti-MERS-CoV Nucleocapsid protein antibody [abd208] ab316330 at 1/200 (5.895 ug/ml) dilution, followed by Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor® 488) antibody at 1/100 (5ug/ml) dilution (Green).
Confocal image showing cytoplasmic staining in 293T cells transfected with MERS-CoV Nucleocapsid expression vector containing a Myc-his-tag® (shown in green). The counterstain was observed in red. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Alexa Fluor® 647 Anti-Myc tag antibody [9E10] ab223895 Anti-Myc tag Mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain at 1/100 (5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor® 488) at 1/100 (5ug/ml) dilution.
![Indirect ELISA - Anti-MERS-CoV Nucleocapsid protein antibody [abd208] - BSA and Azide free (ab316331), expandable thumbnail](https://content.abcam.com/products/images/mers-cov-nucleocapsid-protein-antibody-abd208-bsa-and-azide-free-ab316331--indirect-elisa-img428932.jpg "Indirect ELISA - Anti-MERS-CoV Nucleocapsid protein antibody [abd208] - BSA and Azide free (ab316331)")
Indirect ELISA - Anti-MERS-CoV Nucleocapsid protein antibody [abd208] - BSA and Azide free (ab316331)
This data was developed using Anti-MERS-CoV Nucleocapsid protein antibody [abd208] ab316330, the same antibody clone in a different buffer formulation.
Indirect ELISA analysis of Anti-MERS-CoV Nucleocapsid protein antibody [abd208] ab316330 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Human IgG (H+L) at 1:1000 dilution.
Antigen: HCoV-NL63 Nucleocapsid protein,MERS-CoV Nucleocapsid Protein,Recombinant SARS-CoV Nucleocapsid Protein,Recombinant HCoV-OC43 Nucleocapsid Protein,Human Coronavirus HKU1 Nucleoprotein,Recombinant HCoV-229E Nucleocapsid Protein.
Antigen concentration: 5000 ng/ml
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