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AB316331

Anti-MERS-CoV Nucleocapsid protein antibody [abd208] - BSA and Azide free

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Human Recombinant Monoclonal NCAP antibody. Carrier free. Suitable for WB, ICC/IF, I-ELISA and reacts with Recombinant fragment - MERS-CoV, Transfected cell line - MERS-CoV samples.
3 Images
Immunocytochemistry/ Immunofluorescence - Anti-MERS-CoV Nucleocapsid protein antibody [abd208] - BSA and Azide free (AB316331)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MERS-CoV Nucleocapsid protein antibody [abd208] - BSA and Azide free (AB316331)

This data was developed using ab316330, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) transfected with a MERS-CoV Nucleocapsid protein expression vector containing a myc tag. cells labelling MERS-CoV Nucleocapsid protein with ab316330 at 1/200 (5.895 ug/ml) dilution, followed by Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor® 488) antibody at 1/100 (5ug/ml) dilution (Green).

Confocal image showing cytoplasmic staining in 293T cells transfected with MERS-CoV Nucleocapsid expression vector containing a Myc-his-tag® (shown in green). The counterstain was observed in red. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223895 Anti-Myc tag Mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain at 1/100 (5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor® 488) at 1/100 (5ug/ml) dilution.

Indirect ELISA - Anti-MERS-CoV Nucleocapsid protein antibody [abd208] - BSA and Azide free (AB316331)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-MERS-CoV Nucleocapsid protein antibody [abd208] - BSA and Azide free (AB316331)

This data was developed using ab316330, the same antibody clone in a different buffer formulation.

Indirect ELISA analysis of ab316330 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Human IgG (H+L) at 1 : 1000 dilution.

Antigen : HCoV-NL63 Nucleocapsid protein,MERS-CoV Nucleocapsid Protein,Recombinant SARS-CoV Nucleocapsid Protein,Recombinant HCoV-OC43 Nucleocapsid Protein,Human Coronavirus HKU1 Nucleoprotein,Recombinant HCoV-229E Nucleocapsid Protein.

Antigen concentration : 5000 ng/ml

Western blot - Anti-MERS-CoV Nucleocapsid protein antibody [abd208] - BSA and Azide free (AB316331)
  • WB

Supplier Data

Western blot - Anti-MERS-CoV Nucleocapsid protein antibody [abd208] - BSA and Azide free (AB316331)

This data was developed using ab316330, the same antibody clone in a different buffer formulation.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-MERS-CoV Nucleocapsid protein antibody [abd208] (<a href='/en-us/products/primary-antibodies/mers-cov-nucleocapsid-protein-antibody-abd208-ab316330'>ab316330</a>) at 1/1000 dilution

All lanes:

His-tagged MERS-CoV Nucleocapsid recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Human IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-human-igg-h-l-hrp-ab6858'>ab6858</a>) at 1/5000 dilution

Observed band size: 46 kDa

true

Exposure time: 180s

  • Unconjugated

    Anti-MERS-CoV Nucleocapsid protein antibody [abd208]

Key facts

Host species

Human

Clonality

Monoclonal

Clone number

abd208

Isotype

IgG1

Carrier free

Yes

Reacts with

MERS-CoV

Applications

ICC/IF, I-ELISA, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody has been tested only with recombinant MERS-CoV Nucleocapsid protein in western blot.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "MERS-CoV": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Recombinant fragment - MERS-CoV": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>" }, "Transfected cell line - MERS-CoV": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" } } }
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Product details

ab316331 is the carrirer-free version of ab316330.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MERS-CoV Nucleocapsid protein also called the N protein plays an important role in the assembly process of the Middle East Respiratory Syndrome Coronavirus (MERS-CoV). The N protein is a structural component that packages the viral RNA genome into a helical ribonucleocapsid which assists in protecting the RNA. This protein which has a mass of approximately 48 kDa is expressed during the viral infection in the host cells particularly in the cytoplasm where it facilitates the encapsulation of the viral genome.
Biological function summary

The MERS-CoV Nucleocapsid protein interacts closely with other viral proteins including the membrane (M) protein to ensure successful viral replication and assembly. This protein does not work alone; it is part of the larger ribonucleoprotein complex which plays an essential role in stabilizing the viral RNA. Its interaction with the M protein helps in localizing the N protein to specific sites in the host cell preparing the components necessary for virion assembly and release.

Pathways

The MERS-CoV Nucleocapsid protein engages in processes important for viral pathogenesis and host cell interaction. The protein is part of the coronavirus replication and transcription complex pathway. Within this matrix it associates with non-structural proteins like the RNA-dependent RNA polymerase to enhance RNA synthesis. Additionally it partakes in modulation of host processes to evade the immune response a pivotal aspect of how coronaviruses maintain infection and propagate within the host.

The MERS-CoV Nucleocapsid protein is intimately associated with Middle East Respiratory Syndrome (MERS) a severe respiratory illness. This protein aids the virus in evading the host immune response potentially contributing to disease severity. N protein interactions with host cellular machinery combined with the immune evasion tactics offer insights into viral pathogenicity and might reveal potential therapeutic targets. Understanding these interactions can shed light on how interventions might disrupt the viral life cycle and ameliorate disease impact.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information MERS-CoV Nucleocapsid
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com