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AB317040

Anti-MERS-CoV Spike glycoprotein antibody [abd146]

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Human Recombinant Monoclonal SPIKE antibody. Suitable for ICC/IF, I-ELISA and reacts with Recombinant full length protein - Betacoronavirus England 1 samples.

View Alternative Names

3, S, Spike glycoprotein, S glycoprotein, E2, Peplomer protein

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-MERS-CoV Spike glycoprotein antibody [abd146] (AB317040)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MERS-CoV Spike glycoprotein antibody [abd146] (AB317040)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling MERS-CoV Spike glycoprotein with ab317040 at 1/1000 (1.03 ug/ml) dilution, followed by Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor® 488) antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing cytoplasmic and membranous staining in 293T cells transfected with a MERS-CoV Spike glycoprotein protein expression vector containing a myc-His-tag® (shown in green) but no staining in 293T cells transfected with a SARS-CoV-2 Spike glycoprotein vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 (5ug/ml) dilution (Magenta). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor® 488) at 1/1000 (2 ug/ml dilution).

Indirect ELISA - Anti-MERS-CoV Spike glycoprotein antibody [abd146] (AB317040)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-MERS-CoV Spike glycoprotein antibody [abd146] (AB317040)

Indirect ELISA analysis of ab317040 at 0-2000 ng/ml. The secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Human IgG (H+L) at 1 : 1000 dilution.

Antigen : HCoV-HKU1 spike protein, MERS-CoV Spike protein.

Antigen concentration : 1000 ng/ml.

  • Carrier free

    Anti-MERS-CoV Spike glycoprotein antibody [abd146] - BSA and Azide free

Key facts

Host species

Human

Clonality

Monoclonal

Clone number

abd146

Isotype

IgG

Carrier free

No

Reacts with

MERS-CoV, Betacoronavirus England 1

Applications

I-ELISA, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

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Proteins & Peptides

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Betacoronavirus England 1": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "MERS-CoV": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Betacoronavirus England 1": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p></p>", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "0-2000 ng/mL", "IELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MERS-CoV Spike glycoprotein also known as MERS-CoV S protein serves as an essential element for viral entry into host cells. It has a molecular mass of approximately 135 kDa and is expressed on the surface of the Middle East Respiratory Syndrome Coronavirus (MERS-CoV). This glycoprotein facilitates the attachment of the virus to the host cell receptor specifically the dipeptidyl peptidase 4 (DPP4) initiating the fusion process required for viral entry.
Biological function summary

The Spike glycoprotein of MERS-CoV enables the virus to penetrate host cellular membranes. It functions as a trimeric complex where each monomer consists of two functional subunits S1 and S2. The S1 subunit contains the receptor-binding domain while the S2 subunit is responsible for membrane fusion. The coordination of these activities allows the virus to successfully infect host cells and propagate within the infected organism.

Pathways

The MERS-CoV Spike glycoprotein plays a vital role in the viral entry and fusion pathways. It interacts with the host receptor DPP4 allowing the virus to exploit the host's endosomal and fusion pathways for cellular entry. The S2 subunit's fusion peptide cooperates with other viral proteins such as the viral envelope proteins to enable the fusion of viral and cellular membranes influencing the viral replication cycle significantly.

The MERS-CoV Spike glycoprotein is closely associated with MERS a severe respiratory illness. The interaction of this glycoprotein with DPP4 is central to the pathogenesis of MERS facilitating the virus's ability to infect human hosts and cause disease. Furthermore this glycoprotein's ability to mutate plays a role in the variability of viral infectivity and virulence posing challenges for vaccine and therapeutic developments.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Spike protein S1. Attaches the virion to the cell membrane by interacting with host receptor, initiating the infection (By similarity). Interacts with host DPP4 to mediate virla entry.. Spike protein S2. Mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states : pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.. Spike protein S2'. Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.
See full target information S
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com