Human Recombinant Monoclonal SPIKE antibody. Suitable for ICC/IF, I-ELISA and reacts with Transfected cell line - MERS-CoV, Recombinant fragment - MERS-CoV samples.
Images
![Indirect ELISA - Anti-MERS Spike glycoprotein antibody [abd144] (AB322146), expandable thumbnail](https://content.abcam.com/products/images/mers-spike-glycoprotein-antibody-abd144-ab322146--indirect-elisa-img438920.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-MERS Spike glycoprotein antibody [abd144] (AB322146)")
Key facts
- Isotype
IgG
- Host species
Human
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Reactivity data
Select an application| ICC/IF | I-ELISA | IHC-P | WB | |
|---|---|---|---|---|
| MERS-CoV | Predicted | Predicted | Not recommended | Not recommended |
| Recombinant fragment - MERS-CoV | Not recommended | Tested | Not recommended | Not recommended |
| Transfected cell line - MERS-CoV | Tested | Not recommended | Not recommended | Not recommended |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - MERS-CoV | Dilution info 1/500 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species MERS-CoV | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - MERS-CoV | Dilution info - | Notes - |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - MERS-CoV | Dilution info 2000 ng/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species MERS-CoV | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - MERS-CoV | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species MERS-CoV, Transfected cell line - MERS-CoV, Recombinant fragment - MERS-CoV | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species MERS-CoV, Transfected cell line - MERS-CoV, Recombinant fragment - MERS-CoV | Dilution info - | Notes - |
Human Recombinant Monoclonal SPIKE antibody. Suitable for ICC/IF, I-ELISA and reacts with Transfected cell line - MERS-CoV, Recombinant fragment - MERS-CoV samples.
Key facts
- Isotype
IgG
- Host species
Human
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Clone number
abd144
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Indirect ELISA - Anti-MERS Spike glycoprotein antibody [abd144] (ab322146), expandable thumbnail](https://content.abcam.com/products/images/mers-spike-glycoprotein-antibody-abd144-ab322146--indirect-elisa-img438920.jpg "Indirect ELISA - Anti-MERS Spike glycoprotein antibody [abd144] (ab322146)")
Indirect ELISA - Anti-MERS Spike glycoprotein antibody [abd144] (ab322146)
Indirect ELISA analysis of ab322146 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Human IgG (H+L) at 1:1000 dilution.
Antigen: MERS-CoV Spike protein.
Antigen concentration: 5000 ng/ml
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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