AB70693

Anti-MERTK antibody

Name: Anti-MERTK antibody (ab70693) | Abcam
Brand: Abcam Limited
SKU: AB70693
Price: 535 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(3 Publications)

Rabbit Polyclonal MERTK antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human MERTK.

View Alternative Names

MER, MERTK, Tyrosine-protein kinase Mer, Proto-oncogene c-Mer, Receptor tyrosine kinase MerTK

1 Images

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Western blot - Anti-MERTK antibody (AB70693)

All lanes:

Western blot - Anti-MERTK antibody (ab70693) at 0.1 µg/mL

All lanes:

U937 cell lysate at 50 µg

Predicted band size: 110 kDa

Observed band size: 120 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human MERTK. The exact immunogen used to generate this antibody is proprietary information.

Q12866

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MERTK protein also known as c-Mer tyrosine kinase plays a mechanical role as a receptor tyrosine kinase with a mass of approximately 110 kDa. It is expressed in multiple tissues including the retina immune cells and some epithelial cells. MERTK functions by binding with its ligands facilitating downstream signaling that affects cellular functions. The receptor is part of the TAM family alongside proteins like AXL and TYRO3.

Biological function summary

The receptor MERTK orchestrates processes critical for maintaining cellular homeostasis such as efferocytosis and immune response regulation. It does not operate alone but often interacts with other cellular components to form functional complexes. This protein contributes significantly to phagocytosis a cellular process for clearing apoptotic cells ensuring a controlled immune environment.

Pathways

Several signal transduction pathways involve c-Mer tyrosine kinase in maintaining cellular communication. Notably it participates in the RAS/MAPK and PI3K/AKT pathways which are essential for cell survival and proliferation. These pathways involve interactions with proteins such as GAB1 and SOS highlighting the receptor's integration in cell signaling networks.

Aberrant activity of MERTK has associations with cancer and autoimmune disorders. In cancers such as leukemia overexpression of the MERTK protein promotes cell survival and proliferation. Its interaction with other proteins like GRB2 can further exacerbate oncogenic pathways. In autoimmune diseases dysregulated MERTK expression can lead to improper clearance of apoptotic cells contributing to systemic inflammation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment (PubMed : 32640697). Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Also plays an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.

See full target information MERTK

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceuticals (Basel, Switzerland) 14: PubMed33562150

2021

MERTK Inhibition: Potential as a Treatment Strategy in EGFR Tyrosine Kinase Inhibitor-Resistant Non-Small Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chao-Ju Chen,Yu-Peng Liu

Emerging microbes & infections 9:571-581 PubMed32172658

2020

MERTK is a host factor that promotes classical swine fever virus entry and antagonizes innate immune response in PK-15 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Guanglai Zheng,Lian-Feng Li,Yuexiu Zhang,Liang Qu,Wenjing Wang,Miao Li,Shaoxiong Yu,Mo Zhou,Yuzi Luo,Yuan Sun,Muhammad Munir,Su Li,Hua-Ji Qiu

Cancer research 70:7242-52 PubMed20719884

2010

Crucial roles for protein kinase C isoforms in tumor-specific killing by apoptin.

Applications

Species

Human

Jie Jiang,Daryl Cole,Nigel Westwood,Lee Macpherson,Farzin Farzaneh,Ghulam Mufti,Mahvash Tavassoli,Joop Gäken

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MERTK antibody

Western blot - Anti-MERTK antibody (AB70693)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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