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AB270448

Anti-MERTK antibody [EPR23248-259]

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(1 Publication)

Rabbit Recombinant Monoclonal MERTK antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

MER, MERTK, Tyrosine-protein kinase Mer, Proto-oncogene c-Mer, Receptor tyrosine kinase MerTK

3 Images
Western blot - Anti-MERTK antibody [EPR23248-259] (AB270448)
  • WB

Lab

Western blot - Anti-MERTK antibody [EPR23248-259] (AB270448)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Negative control : HL-60 (PMID : 23474756)

This blot was developed using a higher sensitivity ECL substrate.

Exposure time : 48 seconds

All lanes:

Western blot - Anti-MERTK antibody [EPR23248-259] (ab270448) at 1/1000 dilution

Lane 1:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

HL-60 (human acute promyelocytic leukemia promyeloblast) whole cell lysate at 20 µg

Lane 3:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

U-937 (human histiocytic lymphoma monocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 110 kDa

Observed band size: 150-210 kDa

false

Western blot - Anti-MERTK antibody [EPR23248-259] (AB270448)
  • WB

Lab

Western blot - Anti-MERTK antibody [EPR23248-259] (AB270448)

Exposure time :

Lane 1 : 3 minutes
Lane 2 : 70 seconds

Blocking/Diluting buffer : 5% NFDM/TBST.

This blot was developed using a higher sensitivity ECL substrate.

All lanes:

Western blot - Anti-MERTK antibody [EPR23248-259] (ab270448) at 1/1000 dilution

Lane 1:

Human ovary tissue lysate at 20 µg

Lane 2:

Human testis tissue lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 110 kDa

Observed band size: 150 kDa,150-210 kDa,210 kDa

false

Western blot - Anti-MERTK antibody [EPR23248-259] (AB270448)
  • WB

Lab

Western blot - Anti-MERTK antibody [EPR23248-259] (AB270448)

Blocking and diluting buffer and concentration : 5% NFDM/TBST This blot was developed using a higher sensitivity ECL substrate. Exposure time : Lane 1 : 70 seconds; Lanes 2-4 : 3 minutes.

All lanes:

Western blot - Anti-MERTK antibody [EPR23248-259] (ab270448) at 1/1000 dilution

Lane 1:

Mouse spleen tissue lysate at 20 µg

Lane 2:

Mouse kidney tissue lysate at 20 µg

Lane 3:

Rat spleen tissue lysate at 20 µg

Lane 4:

Rat kidney tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 110 kDa

Observed band size: 150-210 kDa

false

  • Carrier free

    Anti-MERTK antibody [EPR23248-259] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23248-259

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MERTK protein also known as c-Mer tyrosine kinase plays a mechanical role as a receptor tyrosine kinase with a mass of approximately 110 kDa. It is expressed in multiple tissues including the retina immune cells and some epithelial cells. MERTK functions by binding with its ligands facilitating downstream signaling that affects cellular functions. The receptor is part of the TAM family alongside proteins like AXL and TYRO3.
Biological function summary

The receptor MERTK orchestrates processes critical for maintaining cellular homeostasis such as efferocytosis and immune response regulation. It does not operate alone but often interacts with other cellular components to form functional complexes. This protein contributes significantly to phagocytosis a cellular process for clearing apoptotic cells ensuring a controlled immune environment.

Pathways

Several signal transduction pathways involve c-Mer tyrosine kinase in maintaining cellular communication. Notably it participates in the RAS/MAPK and PI3K/AKT pathways which are essential for cell survival and proliferation. These pathways involve interactions with proteins such as GAB1 and SOS highlighting the receptor's integration in cell signaling networks.

Aberrant activity of MERTK has associations with cancer and autoimmune disorders. In cancers such as leukemia overexpression of the MERTK protein promotes cell survival and proliferation. Its interaction with other proteins like GRB2 can further exacerbate oncogenic pathways. In autoimmune diseases dysregulated MERTK expression can lead to improper clearance of apoptotic cells contributing to systemic inflammation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment (PubMed : 32640697). Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Also plays an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.
See full target information MERTK
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:1675 PubMed36966130

2023

Bacterial outer membrane vesicle based versatile nanosystem boosts the efferocytosis blockade triggered tumor-specific immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Wan-Ru Zhuang,Yunfeng Wang,Weidong Nie,Yao Lei,Chao Liang,Jiaqi He,Liping Zuo,Li-Li Huang,Hai-Yan Xie
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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