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Rabbit Monoclonal MERTK antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

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Images

Western blot - Anti-MERTK antibody [EPR26359-12] (AB300136), expandable thumbnail
  • Western blot - Anti-MERTK antibody [EPR26359-12] (AB300136), expandable thumbnail
  • Flow Cytometry - Anti-MERTK antibody [EPR26359-12] (AB300136), expandable thumbnail
  • Flow Cytometry - Anti-MERTK antibody [EPR26359-12] (AB300136), expandable thumbnail
  • Flow Cytometry - Anti-MERTK antibody [EPR26359-12] (AB300136), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFIPFlow Cyt
Human
Tested
Not recommended
Expected
Not recommended
Tested
Mouse
Tested
Tested
Tested
Not recommended
Tested
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Tested

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

For Western Blot, it is recommended to use Anti-MERTK antibody [Y323] ab52968 for detecting human samples and Anti-MERTK antibody [EPR17534-139] ab184086 for detecting mouse samples.

Species

Mouse

Dilution info

1/1000

Notes

For Western Blot, it is recommended to use Anti-MERTK antibody [Y323] ab52968 for detecting human samples and Anti-MERTK antibody [EPR17534-139] ab184086 for detecting mouse samples.

Not recommended
Not recommended

Species

Rat

Dilution info

-

Notes

For Western Blot, it is recommended to use Anti-MERTK antibody [Y323] ab52968 for detecting human samples and Anti-MERTK antibody [EPR17534-139] ab184086 for detecting mouse samples.

Tested
Tested

Species

Mouse

Dilution info

1/800

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Mouse

Dilution info

1/50

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Human, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Species

Mouse

Dilution info

1/50

Notes

-

Species

Rat

Dilution info

1/50

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment (PubMed:32640697). Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Also plays an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.

Alternative names

Recommended products

Rabbit Monoclonal MERTK antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR26359-12

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The MERTK protein also known as c-Mer tyrosine kinase plays a mechanical role as a receptor tyrosine kinase with a mass of approximately 110 kDa. It is expressed in multiple tissues including the retina immune cells and some epithelial cells. MERTK functions by binding with its ligands facilitating downstream signaling that affects cellular functions. The receptor is part of the TAM family alongside proteins like AXL and TYRO3.

Biological function summary

The receptor MERTK orchestrates processes critical for maintaining cellular homeostasis such as efferocytosis and immune response regulation. It does not operate alone but often interacts with other cellular components to form functional complexes. This protein contributes significantly to phagocytosis a cellular process for clearing apoptotic cells ensuring a controlled immune environment.

Pathways

Several signal transduction pathways involve c-Mer tyrosine kinase in maintaining cellular communication. Notably it participates in the RAS/MAPK and PI3K/AKT pathways which are essential for cell survival and proliferation. These pathways involve interactions with proteins such as GAB1 and SOS highlighting the receptor's integration in cell signaling networks.

Associated diseases and disorders

Aberrant activity of MERTK has associations with cancer and autoimmune disorders. In cancers such as leukemia overexpression of the MERTK protein promotes cell survival and proliferation. Its interaction with other proteins like GRB2 can further exacerbate oncogenic pathways. In autoimmune diseases dysregulated MERTK expression can lead to improper clearance of apoptotic cells contributing to systemic inflammation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

  • Western blot - Anti-MERTK antibody [EPR26359-12] (ab300136), expandable thumbnail

    Western blot - Anti-MERTK antibody [EPR26359-12] (ab300136)

    Anti-MERTK antibody [Y323] ab52968 1/1000
    Anti-MERTK antibody [EPR17534-139] ab184086 1/1000
    Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 1/1000000
    Blocking/Diluting buffer and concentration: 5% NFDM/TBST
    For Western Blot, it is recommended to use Anti-MERTK antibody [Y323] ab52968 for detecting human samples and Anti-MERTK antibody [EPR17534-139] ab184086 for detecting mouse samples.

    Lanes 1 - 4: Western blot - Anti-MERTK antibody [EPR26359-12] (ab300136) at 1/1000 dilution

    Lanes 1 - 4: Western blot - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) ab300137)

    Lane 1: HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 2: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: Mouse spleen tissue lysate at 20 µg

    Lane 4: J774A.1 (Mouse reticulum cell sarcoma monocyte macrophage) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 110-140,180-210 kDa

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST
    For Western Blot, it is recommended to use Anti-MERTK antibody [Y323] ab52968 for detecting human samples and Anti-MERTK antibody [EPR17534-139] ab184086 for detecting mouse samples.

  • Western blot - Anti-MERTK antibody [EPR26359-12] (ab300136), expandable thumbnail

    Western blot - Anti-MERTK antibody [EPR26359-12] (ab300136)

    Blocking and dilution buffer:5% NFDM/TBST.

    Exposure time:ECL western blotting substrate 180 seconds, higher sensitivity ECL western substrate 80 seconds.

    The left image uses the ECL western blotting substrate and the right image uses the higher sensitivity ECL western substrate.

    We recommend using a higher sensitive ECL substrate to increase the band intensity.

    All lanes: Western blot - Anti-MERTK antibody [EPR26359-12] (ab300136) at 1/1000 dilution

    Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 2: Mouse spleen tissue lysate at 20 µg

    Lane 3: J774A.1 (mouse reticulum cell sarcoma monocyte macrophage), whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 110 kDa

    Observed band size: 110-140 kDa, 180-210 kDa

    Blocking and dilution buffer:5% NFDM/TBST.

    Exposure time:ECL western blotting substrate 180 seconds, higher sensitivity ECL western substrate 80 seconds.

    The left image uses the ECL western blotting substrate and the right image uses the higher sensitivity ECL western substrate.

    We recommend using a higher sensitive ECL substrate to increase the band intensity.

  • Flow Cytometry - Anti-MERTK antibody [EPR26359-12] (ab300136), expandable thumbnail

    Flow Cytometry - Anti-MERTK antibody [EPR26359-12] (ab300136)

    Flow cytometric analysis of WEHI-231 (mouse B cell lymphoma B lymphocyte, Left) / J774A.1 (mouse reticulum cell sarcoma monocyte macrophage, Right) cells labelling MERTK with ab300136 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti-Rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells. Negative control: WEHI-231(PMID:16652142; PMID:12884290)

  • Flow Cytometry - Anti-MERTK antibody [EPR26359-12] (ab300136), expandable thumbnail

    Flow Cytometry - Anti-MERTK antibody [EPR26359-12] (ab300136)

    Flow cytometric analysis of NR8383 (rat lung macrophage alveolar) cells labelling MERTK with ab300136 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti-Rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

  • Flow Cytometry - Anti-MERTK antibody [EPR26359-12] (ab300136), expandable thumbnail

    Flow Cytometry - Anti-MERTK antibody [EPR26359-12] (ab300136)

    Flow cytometric analysis of HL-60 (human acute promyelocytic leukemia promyeloblast, Left) / HepG2 (human hepatocellular carcinoma epithelial cell, Right) cells labelling MERTK with ab300136 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti-Rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells. Negative control: HL-60 (PMID:23474756). 

  • Immunocytochemistry/ Immunofluorescence - Anti-MERTK antibody [EPR26359-12] (ab300136), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MERTK antibody [EPR26359-12] (ab300136)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized J774A.1(mouse reticulum cell sarcoma monocyte macrophage) cells labeling MERTK with ab300136 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preabsorbed antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in J774A.1 cell line. Negative control: WEHI-231(PMID: 16652142; PMID:12884290).


    Tubulin is detected with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).


    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preabsorbed at 1/1000 dilution.

  • Western blot - Anti-MERTK antibody [EPR26359-12] (ab300136), expandable thumbnail

    Western blot - Anti-MERTK antibody [EPR26359-12] (ab300136)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labelling MERTK with ab300136 at 1/800 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Membranous staining on endothelial cells of mouse kidney (PMID: 29350876) is observed. The section was incubated with ab300136 at 4°C overnight. Counterstained with Hematoxylin.


    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).


    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). 

  • Western blot - Anti-MERTK antibody [EPR26359-12] (ab300136), expandable thumbnail

    Western blot - Anti-MERTK antibody [EPR26359-12] (ab300136)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labelling MERTK with ab300136 at 1/800 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Membranous staining on mouse spleen (PMID: 25479139) is observed. The section was incubated with ab300136 at 4°C overnight. Counterstained with Hematoxylin.


    Secondary antibody only control: PBS was used instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).


    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

  • Western blot - Anti-MERTK antibody [EPR26359-12] (ab300136), expandable thumbnail

    Western blot - Anti-MERTK antibody [EPR26359-12] (ab300136)

    Blocking and dilution buffer and concentration: 5% NFDM/TBST. 


    Negative control: WEHI-231 (PMID: 12884290)
    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 16652142)
    This blot was developed using a higher sensitivity ECL substrate.

    All lanes: Western blot - Anti-MERTK antibody [EPR26359-12] (ab300136) at 1/1000 dilution

    Lane 1: J774A.1 (mouse reticulum cell sarcoma monocyte macrophage) whole cell lysate at 20 µg

    Lane 2: WEHI-231 (mouse B cell lymphoma B lymphocyte) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 110-140 kDa, 180-210 kDa

    Exposure time: 92s

  • Western blot - Anti-MERTK antibody [EPR26359-12] (ab300136), expandable thumbnail

    Western blot - Anti-MERTK antibody [EPR26359-12] (ab300136)

    Blocking and dilution buffer and concentration: 5% NFDM/TBST. 


    Negative control: HL-60 (PMID:23474756)
    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 16652142)
    This blot was developed using a higher sensitivity ECL substrate.

    All lanes: Western blot - Anti-MERTK antibody [EPR26359-12] (ab300136) at 1/1000 dilution

    Lane 1: Mouse spleen tissue lysate at 20 µg

    Lane 2: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: HL-60 (human acute promyelocytic leukemia promyeloblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 110-140 kDa, 180-210 kDa

    Exposure time: 3min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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