Rabbit Monoclonal MERTK antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt and reacts with Human, Mouse, Rat samples. Cited in 2 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
WB | IHC-P | ICC/IF | IP | Flow Cyt | |
---|---|---|---|---|---|
Human | Tested | Not recommended | Expected | Not recommended | Tested |
Mouse | Tested | Tested | Tested | Not recommended | Tested |
Rat | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 | Notes For Western Blot, it is recommended to use Anti-MERTK antibody [Y323] ab52968 for detecting human samples and Anti-MERTK antibody [EPR17534-139] ab184086 for detecting mouse samples. |
Species Mouse | Dilution info 1/1000 | Notes For Western Blot, it is recommended to use Anti-MERTK antibody [Y323] ab52968 for detecting human samples and Anti-MERTK antibody [EPR17534-139] ab184086 for detecting mouse samples. |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes For Western Blot, it is recommended to use Anti-MERTK antibody [Y323] ab52968 for detecting human samples and Anti-MERTK antibody [EPR17534-139] ab184086 for detecting mouse samples. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/800 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/50 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50 | Notes - |
Species Mouse | Dilution info 1/50 | Notes - |
Species Rat | Dilution info 1/50 | Notes - |
Select an associated product type
Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment (PubMed:32640697). Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Also plays an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.
MER, MER, MERTK, Tyrosine-protein kinase Mer, Proto-oncogene c-Mer, Receptor tyrosine kinase MerTK
Rabbit Monoclonal MERTK antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt and reacts with Human, Mouse, Rat samples. Cited in 2 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR26359-12
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
The MERTK protein also known as c-Mer tyrosine kinase plays a mechanical role as a receptor tyrosine kinase with a mass of approximately 110 kDa. It is expressed in multiple tissues including the retina immune cells and some epithelial cells. MERTK functions by binding with its ligands facilitating downstream signaling that affects cellular functions. The receptor is part of the TAM family alongside proteins like AXL and TYRO3.
The receptor MERTK orchestrates processes critical for maintaining cellular homeostasis such as efferocytosis and immune response regulation. It does not operate alone but often interacts with other cellular components to form functional complexes. This protein contributes significantly to phagocytosis a cellular process for clearing apoptotic cells ensuring a controlled immune environment.
Several signal transduction pathways involve c-Mer tyrosine kinase in maintaining cellular communication. Notably it participates in the RAS/MAPK and PI3K/AKT pathways which are essential for cell survival and proliferation. These pathways involve interactions with proteins such as GAB1 and SOS highlighting the receptor's integration in cell signaling networks.
Aberrant activity of MERTK has associations with cancer and autoimmune disorders. In cancers such as leukemia overexpression of the MERTK protein promotes cell survival and proliferation. Its interaction with other proteins like GRB2 can further exacerbate oncogenic pathways. In autoimmune diseases dysregulated MERTK expression can lead to improper clearance of apoptotic cells contributing to systemic inflammation.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Anti-MERTK antibody [Y323] ab52968 1/1000
Anti-MERTK antibody [EPR17534-139] ab184086 1/1000
Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 1/1000000
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
For Western Blot, it is recommended to use Anti-MERTK antibody [Y323] ab52968 for detecting human samples and Anti-MERTK antibody [EPR17534-139] ab184086 for detecting mouse samples.
Lanes 1 - 4: Western blot - Anti-MERTK antibody [EPR26359-12] (ab300136) at 1/1000 dilution
Lanes 1 - 4: Western blot - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) ab300137)
Lane 1: HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 2: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3: Mouse spleen tissue lysate at 20 µg
Lane 4: J774A.1 (Mouse reticulum cell sarcoma monocyte macrophage) whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 110-140,180-210 kDa
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
For Western Blot, it is recommended to use Anti-MERTK antibody [Y323] ab52968 for detecting human samples and Anti-MERTK antibody [EPR17534-139] ab184086 for detecting mouse samples.
Blocking and dilution buffer:5% NFDM/TBST.
Exposure time:ECL western blotting substrate 180 seconds, higher sensitivity ECL western substrate 80 seconds.
The left image uses the ECL western blotting substrate and the right image uses the higher sensitivity ECL western substrate.
We recommend using a higher sensitive ECL substrate to increase the band intensity.
All lanes: Western blot - Anti-MERTK antibody [EPR26359-12] (ab300136) at 1/1000 dilution
Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg
Lane 2: Mouse spleen tissue lysate at 20 µg
Lane 3: J774A.1 (mouse reticulum cell sarcoma monocyte macrophage), whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 110 kDa
Observed band size: 110-140 kDa, 180-210 kDa
Blocking and dilution buffer:5% NFDM/TBST.
Exposure time:ECL western blotting substrate 180 seconds, higher sensitivity ECL western substrate 80 seconds.
The left image uses the ECL western blotting substrate and the right image uses the higher sensitivity ECL western substrate.
We recommend using a higher sensitive ECL substrate to increase the band intensity.
Flow cytometric analysis of WEHI-231 (mouse B cell lymphoma B lymphocyte, Left) / J774A.1 (mouse reticulum cell sarcoma monocyte macrophage, Right) cells labelling MERTK with ab300136 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti-Rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells. Negative control: WEHI-231(PMID:16652142; PMID:12884290)
Flow cytometric analysis of NR8383 (rat lung macrophage alveolar) cells labelling MERTK with ab300136 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti-Rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
Flow cytometric analysis of HL-60 (human acute promyelocytic leukemia promyeloblast, Left) / HepG2 (human hepatocellular carcinoma epithelial cell, Right) cells labelling MERTK with ab300136 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti-Rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells. Negative control: HL-60 (PMID:23474756).
Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized J774A.1(mouse reticulum cell sarcoma monocyte macrophage) cells labeling MERTK with ab300136 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preabsorbed antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in J774A.1 cell line. Negative control: WEHI-231(PMID: 16652142; PMID:12884290).
Tubulin is detected with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preabsorbed at 1/1000 dilution.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labelling MERTK with ab300136 at 1/800 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Membranous staining on endothelial cells of mouse kidney (PMID: 29350876) is observed. The section was incubated with ab300136 at 4°C overnight. Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labelling MERTK with ab300136 at 1/800 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Membranous staining on mouse spleen (PMID: 25479139) is observed. The section was incubated with ab300136 at 4°C overnight. Counterstained with Hematoxylin.
Secondary antibody only control: PBS was used instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Negative control: WEHI-231 (PMID: 12884290)
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 16652142)
This blot was developed using a higher sensitivity ECL substrate.
All lanes: Western blot - Anti-MERTK antibody [EPR26359-12] (ab300136) at 1/1000 dilution
Lane 1: J774A.1 (mouse reticulum cell sarcoma monocyte macrophage) whole cell lysate at 20 µg
Lane 2: WEHI-231 (mouse B cell lymphoma B lymphocyte) whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 110-140 kDa, 180-210 kDa
Exposure time: 92s
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Negative control: HL-60 (PMID:23474756)
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 16652142)
This blot was developed using a higher sensitivity ECL substrate.
All lanes: Western blot - Anti-MERTK antibody [EPR26359-12] (ab300136) at 1/1000 dilution
Lane 1: Mouse spleen tissue lysate at 20 µg
Lane 2: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3: HL-60 (human acute promyelocytic leukemia promyeloblast) whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 110-140 kDa, 180-210 kDa
Exposure time: 3min
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