AB300137

Anti-MERTK antibody [EPR26359-12] (BSA and Azide free)

BOND RX™ Validated
RabMAb
Recombinant
KO Validated
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Rabbit Recombinant Monoclonal MERTK antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt and reacts with Human, Mouse, Rat samples.

View Alternative Names

MER, MERTK, Tyrosine-protein kinase Mer, Proto-oncogene c-Mer, Receptor tyrosine kinase MerTK

12 Images

Flow Cytometry - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)

Flow cytometric analysis of HL-60 (human acute promyelocytic leukemia promyeloblast, Left) / HepG2 (human hepatocellular carcinoma epithelial cell, Right) cells labelling MERTK with ab300136 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells. Negative control : HL-60 (PMID : 23474756).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)

This data was developed using ab300136, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) Kidney tissue from wild-type C57BL/6J mice and (B) Kidney tissue from MERTK knockout mice tissue labeling MERTK with ab300136 at 1/1000 dilution.

Positive staining on (A) Kidney tissue from wild-type C57BL/6J mice, no staining on (B) Kidney tissue from MERTK knockout mice.

The primary antibody was incubated for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and MERTK-KO homozygous mice (Strain ID : T012765).

Immunocytochemistry/ Immunofluorescence - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)

This data was developed using ab300136, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized J774A.1(mouse reticulum cell sarcoma monocyte macrophage) cells labeling MERTK with ab300136 at 1/50 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preabsorbed antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in J774A.1 cell line. Negative control : WEHI-231(PMID : 16652142; PMID : 12884290).Tubulin is detected with ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preabsorbed at 1/1000 dilution.

Flow Cyt

Supplier Data

Flow Cytometry - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)

This data was developed using ab300136, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of WEHI-231 (mouse B cell lymphoma B lymphocyte, Left) / J774A.1 (mouse reticulum cell sarcoma monocyte macrophage, Right) cells labelling MERTK with ab300136 at 1/50 dilution (1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.Negative control : WEHI-231(PMID : 16652142; PMID : 12884290)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)

This data was developed using ab300136, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of NR8383 (rat lung macrophage alveolar) cells labelling MERTK with ab300136 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

Lab

Western blot - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)

Blocking and dilution buffer : 5% NFDM /TBST.

Exposure time : ECL western blotting substrate 180 seconds, higher sensitivity ECL western substrate 80 seconds.

The left image uses the ECL western blotting substrate 180 seconds and the right image uses the higher sensitivity ECL western substrate 80 seconds.

We recommend using a higher sensitive ECL substrate to increase the band intensity.

This data was developed using ab300136, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MERTK antibody [EPR26359-12] (<a href='/en-us/products/primary-antibodies/mertk-antibody-epr26359-12-ab300136'>ab300136</a>) at 1/1000 dilution

Lane 1:

HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

Mouse spleen tissue lysate at 20 µg

Lane 3:

J774A.1 (mouse reticulum cell sarcoma monocyte macrophage), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 110 kDa

Observed band size: 110-140 kDa,180-210 kDa

false

Lab

Western blot - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)

This data was developed using ab300136, the same antibody clone in a different buffer formulation. Blocking/Diluting buffer and concentration : 5% NFDM/TBST For Western Blot, it is recommended to use ab52968 for detecting human samples and ab184086 for detecting mouse samples.

All lanes:

Western blot - Anti-MERTK antibody [EPR26359-12] (<a href='/en-us/products/primary-antibodies/mertk-antibody-epr26359-12-ab300136'>ab300136</a>) at 1/1000 dilution

Lane 1:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

Mouse spleen tissue lysate at 20 µg

Lane 4:

J774A.1 (Mouse reticulum cell sarcoma monocyte macrophage) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 110-140 kDa,180-210 kDa

false

Supplier Data

Western blot - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)

This data was developed using ab300136, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Negative control : HL-60 (PMID : 23474756)

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 16652142).

All lanes:

Western blot - Anti-MERTK antibody [EPR26359-12] (<a href='/en-us/products/primary-antibodies/mertk-antibody-epr26359-12-ab300136'>ab300136</a>) at 1/1000 dilution

Lane 1:

Mouse spleen tissue lysate at 20 µg

Lane 2:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

HL-60 (human acute promyelocytic leukemia promyeloblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 110-140 kDa,180-210 kDa

true

Exposure time: 3min

Supplier Data

Western blot - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)

This data was developed using ab300136, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labelling MERTK with ab300136 at 1/800 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Membranous staining on mouse spleen (PMID : 25479139) is observed. The section was incubated with ab300136 at 4°C overnight. Counterstained with Hematoxylin.Secondary antibody only control : PBS was used instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Supplier Data

Western blot - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)

This data was developed using ab300136, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labelling MERTK with ab300136 at 1/800 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Membranous staining on endothelial cells of mouse kidney (PMID : 29350876) is observed. The section was incubated with ab300136 at 4°C overnight. Counterstained with Hematoxylin.Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Supplier Data

Western blot - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)

This data was developed using ab300136, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Negative control : WEHI-231 (PMID : 12884290).

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 16652142).

This blot was developed using a higher sensitivity ECL substrate.

All lanes:

Western blot - Anti-MERTK antibody [EPR26359-12] (<a href='/en-us/products/primary-antibodies/mertk-antibody-epr26359-12-ab300136'>ab300136</a>) at 1/1000 dilution

Lane 1:

J774A.1 (mouse reticulum cell sarcoma monocyte macrophage) whole cell lysate at 20 µg

Lane 2:

WEHI-231 (mouse B cell lymphoma B lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 110-140 kDa,180-210 kDa

false

Exposure time: 92s

Lab

Western blot - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)

This data was developed using ab300136, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Mertk-KO homozygous mice (Strain ID : T012765).

All lanes:

Western blot - Anti-MERTK antibody [EPR26359-12] (<a href='/en-us/products/primary-antibodies/mertk-antibody-epr26359-12-ab300136'>ab300136</a>) at 1/1000 dilution

Lane 1:

Wild-type mouse kidney tissue lysate at 40 µg

Lane 2:

Mertk knockout mouse kidney (case 1) tissue lysate at 40 µg

Lane 3:

Mertk knockout mouse kidney (case 2) tissue lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 110-140 kDa

false

Exposure time: 37s

Unconjugated

Anti-MERTK antibody [EPR26359-12]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-MERTK antibody [EPR26359-12]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26359-12

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

IHC-P, WB, Flow Cyt, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "For Western Blot, it is recommended to use <a href='/en-us/products/primary-antibodies/mertk-antibody-y323-ab52968'>ab52968</a> for detecting human samples and <a href='/en-us/products/primary-antibodies/mertk-antibody-epr17534-139-ab184086'>ab184086</a> for detecting mouse samples.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "For Western Blot, it is recommended to use <a href='/en-us/products/primary-antibodies/mertk-antibody-y323-ab52968'>ab52968</a> for detecting human samples and <a href='/en-us/products/primary-antibodies/mertk-antibody-epr17534-139-ab184086'>ab184086</a> for detecting mouse samples.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "For Western Blot, it is recommended to use <a href='/en-us/products/primary-antibodies/mertk-antibody-y323-ab52968'>ab52968</a> for detecting human samples and <a href='/en-us/products/primary-antibodies/mertk-antibody-epr17534-139-ab184086'>ab184086</a> for detecting mouse samples.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MERTK protein also known as c-Mer tyrosine kinase plays a mechanical role as a receptor tyrosine kinase with a mass of approximately 110 kDa. It is expressed in multiple tissues including the retina immune cells and some epithelial cells. MERTK functions by binding with its ligands facilitating downstream signaling that affects cellular functions. The receptor is part of the TAM family alongside proteins like AXL and TYRO3.

Biological function summary

The receptor MERTK orchestrates processes critical for maintaining cellular homeostasis such as efferocytosis and immune response regulation. It does not operate alone but often interacts with other cellular components to form functional complexes. This protein contributes significantly to phagocytosis a cellular process for clearing apoptotic cells ensuring a controlled immune environment.

Pathways

Several signal transduction pathways involve c-Mer tyrosine kinase in maintaining cellular communication. Notably it participates in the RAS/MAPK and PI3K/AKT pathways which are essential for cell survival and proliferation. These pathways involve interactions with proteins such as GAB1 and SOS highlighting the receptor's integration in cell signaling networks.

Aberrant activity of MERTK has associations with cancer and autoimmune disorders. In cancers such as leukemia overexpression of the MERTK protein promotes cell survival and proliferation. Its interaction with other proteins like GRB2 can further exacerbate oncogenic pathways. In autoimmune diseases dysregulated MERTK expression can lead to improper clearance of apoptotic cells contributing to systemic inflammation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment (PubMed : 32640697). Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Also plays an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.

See full target information MERTK

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MERTK antibody [EPR26359-12] (BSA and Azide free)

Flow Cytometry - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)

Immunocytochemistry/ Immunofluorescence - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)

Flow Cytometry - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)

Flow Cytometry - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)

Western blot - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)

Western blot - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)

Western blot - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)

Western blot - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)

Western blot - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)

Western blot - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)

Western blot - Anti-MERTK antibody [EPR26359-12] (BSA and Azide free) (AB300137)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise