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Mouse Monoclonal MERTK antibody. Suitable for Protein Array, IHC-P and reacts with Recombinant full length protein - Human, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MERTK aa 50-150.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [MERTK/3022] (AB268030), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [MERTK/3022] (AB268030), expandable thumbnail
  • Protein Array - Anti-MERTK antibody [MERTK/3022] (AB268030), expandable thumbnail

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human MERTK aa 50-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q12866

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Protein ArrayIHC-P
Human
Expected
Tested
Recombinant full length protein - Human
Tested
Not recommended

Tested
Tested

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1-2 µg/mL
Notes

Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment (PubMed:32640697). Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Also plays an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.

Alternative names

MER, MERTK, Tyrosine-protein kinase Mer, Proto-oncogene c-Mer, Receptor tyrosine kinase MerTK

Recommended products

Mouse Monoclonal MERTK antibody. Suitable for Protein Array, IHC-P and reacts with Recombinant full length protein - Human, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MERTK aa 50-150.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human MERTK aa 50-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q12866
Clone number
MERTK/3022
Purification technique
Affinity purification Protein A/G
Light chain type
kappa
Concentration
Loading...
Purification notes

Purified from Bioreactor concentrate

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The MERTK protein also known as c-Mer tyrosine kinase plays a mechanical role as a receptor tyrosine kinase with a mass of approximately 110 kDa. It is expressed in multiple tissues including the retina immune cells and some epithelial cells. MERTK functions by binding with its ligands facilitating downstream signaling that affects cellular functions. The receptor is part of the TAM family alongside proteins like AXL and TYRO3.

Biological function summary

The receptor MERTK orchestrates processes critical for maintaining cellular homeostasis such as efferocytosis and immune response regulation. It does not operate alone but often interacts with other cellular components to form functional complexes. This protein contributes significantly to phagocytosis a cellular process for clearing apoptotic cells ensuring a controlled immune environment.

Pathways

Several signal transduction pathways involve c-Mer tyrosine kinase in maintaining cellular communication. Notably it participates in the RAS/MAPK and PI3K/AKT pathways which are essential for cell survival and proliferation. These pathways involve interactions with proteins such as GAB1 and SOS highlighting the receptor's integration in cell signaling networks.

Associated diseases and disorders

Aberrant activity of MERTK has associations with cancer and autoimmune disorders. In cancers such as leukemia overexpression of the MERTK protein promotes cell survival and proliferation. Its interaction with other proteins like GRB2 can further exacerbate oncogenic pathways. In autoimmune diseases dysregulated MERTK expression can lead to improper clearance of apoptotic cells contributing to systemic inflammation.

Product promise

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3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [MERTK/3022] (ab268030), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [MERTK/3022] (ab268030)

    Formalin-fixed, paraffin-embedded human Colon Carcinoma stained for MERTK using ab268030 at 2 μg/ml for 30 minutes at RT in immunohistochemical analysis.

    Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [MERTK/3022] (ab268030), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [MERTK/3022] (ab268030)

    Formalin-fixed, paraffin-embedded human Colon Carcinoma stained for MERTK using ab268030 at 2 μg/ml for 30 minutes at RT in immunohistochemical analysis.

    Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes.

  • Protein Array - Anti-MERTK antibody [MERTK/3022] (ab268030), expandable thumbnail

    Protein Array - Anti-MERTK antibody [MERTK/3022] (ab268030)

    Protein Array containing more than 19,000 full-length human proteins using ab268030. Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

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Product protocols

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