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Rabbit Recombinant Monoclonal MERTK antibody. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Human samples. Cited in 61 publications.


Images

Western blot - Anti-MERTK antibody [Y323] (AB52968), expandable thumbnail
  • Immunoprecipitation - Anti-MERTK antibody [Y323] (AB52968), expandable thumbnail
  • Western blot - Anti-MERTK antibody [Y323] (AB52968), expandable thumbnail
  • Western blot - Anti-MERTK antibody [Y323] (AB52968), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [Y323] (AB52968), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBICC/IFIHC-FrIHC-P
Human
Tested
Not recommended
Tested
Not recommended
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/20
Notes

For unpurified use at 1/50.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

(paraformaldehyde or methanol fixed cells)

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species
Human
Dilution info
1/2000
Notes

For unpurified use at 1/500.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

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1 product for Alternative Version

Target data

Function

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment (PubMed:32640697). Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Also plays an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.

Alternative names

MER, MERTK, Tyrosine-protein kinase Mer, Proto-oncogene c-Mer, Receptor tyrosine kinase MerTK

Recommended products

Rabbit Recombinant Monoclonal MERTK antibody. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Human samples. Cited in 61 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
Y323
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The MERTK protein also known as c-Mer tyrosine kinase plays a mechanical role as a receptor tyrosine kinase with a mass of approximately 110 kDa. It is expressed in multiple tissues including the retina immune cells and some epithelial cells. MERTK functions by binding with its ligands facilitating downstream signaling that affects cellular functions. The receptor is part of the TAM family alongside proteins like AXL and TYRO3.

Biological function summary

The receptor MERTK orchestrates processes critical for maintaining cellular homeostasis such as efferocytosis and immune response regulation. It does not operate alone but often interacts with other cellular components to form functional complexes. This protein contributes significantly to phagocytosis a cellular process for clearing apoptotic cells ensuring a controlled immune environment.

Pathways

Several signal transduction pathways involve c-Mer tyrosine kinase in maintaining cellular communication. Notably it participates in the RAS/MAPK and PI3K/AKT pathways which are essential for cell survival and proliferation. These pathways involve interactions with proteins such as GAB1 and SOS highlighting the receptor's integration in cell signaling networks.

Associated diseases and disorders

Aberrant activity of MERTK has associations with cancer and autoimmune disorders. In cancers such as leukemia overexpression of the MERTK protein promotes cell survival and proliferation. Its interaction with other proteins like GRB2 can further exacerbate oncogenic pathways. In autoimmune diseases dysregulated MERTK expression can lead to improper clearance of apoptotic cells contributing to systemic inflammation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

  • Western blot - Anti-MERTK antibody [Y323] (ab52968), expandable thumbnail

    Western blot - Anti-MERTK antibody [Y323] (ab52968)

    ab52968 used at a 1/200 dilution, 18 hours at 4°C, 5% BSA in Tween PBS.

    Positive cell line: A172

    Negative cell lines: CV1 and HeLa.

    Secondary used is a goat anti-rabbit polyclonal-HRP at 1/10000 dilution.

    Blocked with 5% milk for 1 hour at RT.

    All lanes: Western blot - Anti-MERTK antibody [Y323] (ab52968)

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 110 kDa

    Exposure time: 30s

  • Immunoprecipitation - Anti-MERTK antibody [Y323] (ab52968), expandable thumbnail

    Immunoprecipitation - Anti-MERTK antibody [Y323] (ab52968)

    Lane 1: ab52968 (purified) at 1/20 immunoprecipitating MERTK in HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate.

    Lane 2 - PBS.

    For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-MERTK antibody [Y323] (ab52968)

    Predicted band size: 110 kDa

    Observed band size: 140-180 kDa

  • Western blot - Anti-MERTK antibody [Y323] (ab52968), expandable thumbnail

    Western blot - Anti-MERTK antibody [Y323] (ab52968)

    Lanes 1 - 2: Merged signal (red and green). Green - ab52968 observed at 110 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    ab52968 was shown to specifically react with MERTK in wild-type HAP1 cells as signal was lost in MERTK knockout cells. Wild-type and MERTK knockout samples were subjected to SDS-PAGE. The membrane was blocked with 0. ab52968 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-MERTK antibody [Y323] (ab52968) at 1/500 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: MERTK knockout HAP1 whole cell lysate at 20 µg

    Predicted band size: 110 kDa

  • Western blot - Anti-MERTK antibody [Y323] (ab52968), expandable thumbnail

    Western blot - Anti-MERTK antibody [Y323] (ab52968)

    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-MERTK antibody [Y323] (ab52968) at 1/2000 dilution

    Lane 1: HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate at 20 µg

    Lane 2: K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate at 20 µg

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG at 1/1000 dilution

    Predicted band size: 110 kDa

    Observed band size: 140-180 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [Y323] (ab52968), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [Y323] (ab52968)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labeling MERTK with purified ab52968 at 1/500.

    Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Counterstained with hematoxylin.

    Negative control using PBS instead of primary antibody (Inset).

  • Immunohistochemistry (Frozen sections) - Anti-MERTK antibody [Y323] (ab52968), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-MERTK antibody [Y323] (ab52968)

    IHC image of MERTK staining in a section of frozen normal human spleen performed on a Leica BONDTM system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab52968, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [Y323] (ab52968), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [Y323] (ab52968)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymphoma tissue labeling MERTK with unpurified ab52968 at a 1/50 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-MERTK antibody [Y323] (ab52968), expandable thumbnail

    Western blot - Anti-MERTK antibody [Y323] (ab52968)

    All lanes: Western blot - Anti-MERTK antibody [Y323] (ab52968) at 1/1000 dilution

    All lanes: HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate at 10 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 110 kDa

    Observed band size: 180 kDa

  • Western blot - Anti-MERTK antibody [Y323] (ab52968), expandable thumbnail

    Western blot - Anti-MERTK antibody [Y323] (ab52968)

    Western blot: Anti-MERTK antibody [Y323] (ab52968) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab52968 was shown to bind specifically to MERTK. A band was observed at 160 kDa in wild-type A549 cell lysates with no signal observed at this size in MERTK knockout cell line. To generate this image, wild-type and MERTK knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-MERTK antibody [Y323] (ab52968) at 1/1000 dilution

    Lane 1: Wild-type A549 cell lysate at 20 µg

    Lane 2: MERTK knockout A549 cell lysate at 20 µg

    Lane 2: Western blot - Human MERTK knockout A549 cell line (Human MERTK knockout A549 cell line ab301074)

    Lane 3: HAP1 cell lysate at 20 µg

    Lane 4: HeLa cell lysate at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 160 kDa

  • Western blot - Anti-MERTK antibody [Y323] (ab52968), expandable thumbnail

    Image collected and cropped by CiteAb under a CC-BY license from the publication

    Western blot - Anti-MERTK antibody [Y323] (ab52968)

    MERTK western blot using anti-MERTK antibody [Y323] ab52968. Publication image and figure legend from Frejno, M., Zenezini Chiozzi, R., et al., 2017, Mol Syst Biol, PubMed 29101300.


    ab52968 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab52968 please see the product overview.

    MERTK is a prognostic marker in CRC patientsWestern blot visualising MERTK expression in two negative (RKO and CC07) and two positive control cell lines (CaCo‐2 and HT55).IHC staining of MERTK expression in RKO (negative control) and CaCo‐2 (positive control) cells.IHC staining of three representative TMAs from patients enrolled in the QUASAR 2 clinical trial and the corresponding quantification of the signal by pathologists. Arrowheads indicate tumour cells positive for MERTK.Kaplan–Meier plots showing worse overall, disease‐free and recurrence‐free survival of patients with high cytoplasmic/membranous expression of MERTK. P‐values indicate the significance of MERTK as a predictor based on the Wald statistic.

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