Rabbit Recombinant Monoclonal MERTK antibody. Carrier free. Suitable for IHC-Fr, IP, WB, IHC-P and reacts with Human samples. Cited in 2 publications.
Images
![Immunoprecipitation - Anti-MERTK antibody [Y323] - Low endotoxin, Azide free (AB176887), expandable thumbnail](https://content.abcam.com/products/images/mertk-antibody-y323-low-endotoxin-azide-free-ab176887--immunoprecipitation-img10765.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-MERTK antibody [Y323] - Low endotoxin, Azide free (AB176887)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [Y323] - Low endotoxin, Azide free (AB176887), expandable thumbnail](https://content.abcam.com/products/images/mertk-antibody-y323-low-endotoxin-azide-free-ab176887--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img74200.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [Y323] - Low endotoxin, Azide free (AB176887)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [Y323] - Low endotoxin, Azide free (AB176887), expandable thumbnail](https://content.abcam.com/products/images/mertk-antibody-y323-low-endotoxin-azide-free-ab176887--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img24051.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [Y323] - Low endotoxin, Azide free (AB176887)")
![Western blot - Anti-MERTK antibody [Y323] - Low endotoxin, Azide free (AB176887), expandable thumbnail](https://content.abcam.com/products/images/mertk-antibody-y323-low-endotoxin-azide-free-ab176887--western-blot-img433018.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MERTK antibody [Y323] - Low endotoxin, Azide free (AB176887)")
Publications
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- Cell reports 42:1125132023Tissue-resident, extravascular Ly6c monocytes are critical for inflammation in the synovium.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna B Montgomery,Shang Yang Chen,Yidan Wang,Gaurav Gadhvi,Maximilian G Mayr,Carla M Cuda,Salina Dominguez,Hadijat-Kubura Moradeke Makinde,Miranda G Gurra,Alexander V Misharin,Arthur M Mandelin,Eric M Ruderman,Anjali Thakrar,Simran Brar,Mary Carns,Kathleen Aren,Mahzad Akbarpour,Andrew Filer,Saba Nayar,Ana Teososio,Triin Major,Ankit Bharat,G R Scott Budinger,Deborah R Winter,Harris PerlmanPubMed 37204925
- Frontiers in immunology 9:7422018Protective Role of the MER Tyrosine Kinase Efferocytosis in Rheumatoid Arthritis Models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesClaire E J Waterborg et. al.PubMed 29706963
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-Fr | IP | Flow Cyt | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested | Not recommended | Tested |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes (paraformaldehyde or methanol fixed cells) ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes For unpurified use at 1/500. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
13 products for Alternative Product
6 products for Alternative Version
Target data
Function
Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment (PubMed:32640697). Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Also plays an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.
Alternative names
MER, MERTK, Tyrosine-protein kinase Mer, Proto-oncogene c-Mer, Receptor tyrosine kinase MerTK
Recommended products
Rabbit Recombinant Monoclonal MERTK antibody. Carrier free. Suitable for IHC-Fr, IP, WB, IHC-P and reacts with Human samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- Y323
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab176887 is the carrier-free version of Anti-MERTK antibody [Y323] ab52968.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
What does low endotoxin mean?
Our low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The MERTK protein also known as c-Mer tyrosine kinase plays a mechanical role as a receptor tyrosine kinase with a mass of approximately 110 kDa. It is expressed in multiple tissues including the retina immune cells and some epithelial cells. MERTK functions by binding with its ligands facilitating downstream signaling that affects cellular functions. The receptor is part of the TAM family alongside proteins like AXL and TYRO3.
- Biological function summary
The receptor MERTK orchestrates processes critical for maintaining cellular homeostasis such as efferocytosis and immune response regulation. It does not operate alone but often interacts with other cellular components to form functional complexes. This protein contributes significantly to phagocytosis a cellular process for clearing apoptotic cells ensuring a controlled immune environment.
- Pathways
Several signal transduction pathways involve c-Mer tyrosine kinase in maintaining cellular communication. Notably it participates in the RAS/MAPK and PI3K/AKT pathways which are essential for cell survival and proliferation. These pathways involve interactions with proteins such as GAB1 and SOS highlighting the receptor's integration in cell signaling networks.
- Associated diseases and disorders
Aberrant activity of MERTK has associations with cancer and autoimmune disorders. In cancers such as leukemia overexpression of the MERTK protein promotes cell survival and proliferation. Its interaction with other proteins like GRB2 can further exacerbate oncogenic pathways. In autoimmune diseases dysregulated MERTK expression can lead to improper clearance of apoptotic cells contributing to systemic inflammation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunoprecipitation - Anti-MERTK antibody [Y323] - Low endotoxin, Azide free (ab176887), expandable thumbnail](https://content.abcam.com/products/images/mertk-antibody-y323-low-endotoxin-azide-free-ab176887--immunoprecipitation-img10765.jpg "Immunoprecipitation - Anti-MERTK antibody [Y323] - Low endotoxin, Azide free (ab176887)")
Immunoprecipitation - Anti-MERTK antibody [Y323] - Low endotoxin, Azide free (ab176887)
Lane 1: Anti-MERTK antibody [Y323] ab52968 (purified) at 1/20 immunoprecipitating MERTK in HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate.Lane 2 - PBS.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MERTK antibody [Y323] ab52968).
All lanes: Immunoprecipitation - Anti-MERTK antibody [Y323] (Anti-MERTK antibody [Y323] ab52968)
Predicted band size: 110 kDa
Observed band size: 140-180 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [Y323] - Low endotoxin, Azide free (ab176887), expandable thumbnail](https://content.abcam.com/products/images/mertk-antibody-y323-low-endotoxin-azide-free-ab176887--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img74200.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [Y323] - Low endotoxin, Azide free (ab176887)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [Y323] - Low endotoxin, Azide free (ab176887)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labeling MERTK with purified Anti-MERTK antibody [Y323] ab52968 at 1/500.
Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Counterstained with hematoxylin.
Negative control using PBS instead of primary antibody (Inset).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MERTK antibody [Y323] ab52968).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [Y323] - Low endotoxin, Azide free (ab176887), expandable thumbnail](https://content.abcam.com/products/images/mertk-antibody-y323-low-endotoxin-azide-free-ab176887--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img24051.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [Y323] - Low endotoxin, Azide free (ab176887)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK antibody [Y323] - Low endotoxin, Azide free (ab176887)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymphoma tissue labeling MERTK with unpurified Anti-MERTK antibody [Y323] ab52968 at a 1/50 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MERTK antibody [Y323] ab52968).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Western blot - Anti-MERTK antibody [Y323] - Low endotoxin, Azide free (ab176887), expandable thumbnail](https://content.abcam.com/products/images/mertk-antibody-y323-low-endotoxin-azide-free-ab176887--western-blot-img433018.jpg "Western blot - Anti-MERTK antibody [Y323] - Low endotoxin, Azide free (ab176887)")
Western blot - Anti-MERTK antibody [Y323] - Low endotoxin, Azide free (ab176887)
This data was developed using the same antibody clone in a different buffer formulation (Anti-MERTK antibody [Y323] ab52968 ).
Western blot: Anti-MERTK antibody [Y323] (Anti-MERTK antibody [Y323] ab52968) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, Anti-MERTK antibody [Y323] ab52968 was shown to bind specifically to MERTK. A band was observed at 160 kDa in wild-type A549 cell lysates with no signal observed at this size in MERTK knockout cell line. To generate this image, wild-type and MERTK knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-MERTK antibody [Y323] (Anti-MERTK antibody [Y323] ab52968) at 1/1000 dilution
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: MERTK knockout A549 cell lysate at 20 µg
Lane 2: Western blot - Human MERTK knockout A549 cell line (Human MERTK knockout A549 cell line ab301074)
Lane 3: HAP1 cell lysate at 20 µg
Lane 4: HeLa cell lysate at 20 µg
SecondaryAll lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 160 kDa
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