AB192649

Anti-MERTK (phospho Y749) + TYRO3 (phospho Y681) antibody

Name: Anti-MERTK (phospho Y749) + TYRO3 (phospho Y681) antibody (ab192649) | Abcam
Brand: Abcam Limited
SKU: AB192649
Price: 425 USD
Availability: InStock

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(14 Publications)

Rabbit Polyclonal MERTK phospho Y749 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Human MERTK pY749.

View Alternative Names

MER, MERTK, Tyrosine-protein kinase Mer, Proto-oncogene c-Mer, Receptor tyrosine kinase MerTK, BYK, DTK, RSE, SKY, TIF, TYRO3, Tyrosine-protein kinase receptor TYRO3, Tyrosine-protein kinase BYK, Tyrosine-protein kinase DTK, Tyrosine-protein kinase RSE, Tyrosine-protein kinase SKY, Tyrosine-protein kinase TIF

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK (phospho Y749) + TYRO3 (phospho Y681) antibody (AB192649)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MERTK (phospho Y749) + TYRO3 (phospho Y681) antibody (AB192649)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling MERTK (phospho Y749) and TYRO3 (phospho Y681) with ab192649 at 1/50 dilution (left image) or the same antibody preincubated with blocking peptide (right image).

Supplier Data

Western blot - Anti-MERTK (phospho Y749) + TYRO3 (phospho Y681) antibody (AB192649)

All lanes:

Western blot - Anti-MERTK (phospho Y749) + TYRO3 (phospho Y681) antibody (ab192649) at 1/500 dilution

Lane 1:

3T3 cell extract treated with EGF

Lane 2:

3T3 cell extract treated with EGF with antigen-specific peptide

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human MERTK pY749. The exact immunogen used to generate this antibody is proprietary information.

Q12866

Specificity

ab192649 endogenous levels of MERTK only when phosphorylated at Tyrosine 749 and endogenous levels of TYRO3 only when phosphorylated at Tyrosine 681.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab192649 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibody was removed by chromatogramphy using non-phosphopeptide.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-80°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MERTK and TYRO3 are receptor tyrosine kinases that belong to the TAM (TYRO3 AXL MERTK) family. MERTK is often referred to as c-MERTK and TYRO3 as RSE or Sky. MERTK has a molecular weight of approximately 110 kDa and TYRO3 weighs around 105 kDa. MERTK and TYRO3 are mostly found in the immune system particularly in macrophages dendritic cells and also in retinal pigment epithelial cells. These receptors are important for regulating cellular processes including phagocytosis immunological homeostasis and tissue repair.

Biological function summary

These proteins play roles beyond membrane receptors and are pivotal for immune regulation and maintaining cell health. MERTK pairs with ligands such as Gas6 and Protein S leading to receptor-autophosphorylation and activating downstream signaling pathways. TYRO3 also operates in conjunction with similar ligands and both receptors form part of a complex that helps in cellular homeostasis and inflammation control. They contribute to processes like the apoptotic cell clearance which helps in resolving inflammation.

Pathways

Both proteins participate in significant signaling axes such as the PI3K/AKT and MAPK pathways. These pathways are linked with cell survival proliferation and differentiation. MERTK activates these pathways upon binding with its ligands influencing immune functions and cellular repair. Alyso TYRO3 associates closely with Ras and Erk proteins which are central parts of these signaling cascades impacting cellular cycles and apoptosis.

MERTK and TYRO3 have been implicated in conditions like cancer and autoimmune diseases. MERTK overexpression for instance correlates with tumor development and progression making it a target for cancer therapies. TYRO3's involvement in autoimmune diseases roots from its role in immune system modulation. By interacting with AXL another TAM family member both proteins influence conditions like leukemia and rheumatoid arthritis through their control over immune responses and cell survival mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment (PubMed : 32640697). Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Also plays an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.

See full target information MERTK pY749

Additional targets

TYRO3 phospho Y681

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:15583 PubMed40320408

2025

Irisin suppressed the progression of TBI via modulating AMPK/MerTK/autophagy and SYK/ROS/inflammatory signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Kuo Zhang,Yihui Du,Sihui Yang,Guozhu Sun

Science advances 10:eado8366 PubMed39365866

2024

MerTK macrophages promote melanoma progression and immunotherapy resistance through AhR-ALKAL1 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Naming Wu,Jun Li,Lu Li,Liu Yang,Liyun Dong,Chen Shen,Shanshan Sha,Yangxue Fu,Enzhu Dong,Fang Zheng,Zheng Tan,Juan Tao

Nature cardiovascular research 3:28-45 PubMed39195894

2024

ATF3 coordinates the survival and proliferation of cardiac macrophages and protects against ischemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yihui Shao,Yang Li,Yan Liu,Shuolin Zhu,Jianing Wu,Ke Ma,Guoqi Li,Shan Huang,Haichu Wen,Congcong Zhang,Xin-Liang Ma,Ping Li,Jie Du,Yulin Li

Redox biology 64:102763 PubMed37354827

2023

NOX2 inhibition stabilizes vulnerable plaques by enhancing macrophage efferocytosis via MertK/PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Wang,Xin-Yan Liu,Yue Wang,Wen-Xin Zhao,Fa-Dong Li,Peng-Rong Guo,Qian Fan,Xiao-Fan Wu

International journal of molecular sciences 23: PubMed35163068

2022

High Glucose Impairs Expression and Activation of MerTK in ARPE-19 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Alessandra Puddu,Silvia Ravera,Isabella Panfoli,Nadia Bertola,Davide Maggi

Cells 10: PubMed33803290

2021

Growth Arrest-Specific Gene 6 Administration Ameliorates Sepsis-Induced Organ Damage in Mice and Reduces ROS Formation In Vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Livia Salmi,Francesco Gavelli,Filippo Patrucco,Mattia Bellan,Pier Paolo Sainaghi,Gian Carlo Avanzi,Luigi Mario Castello

Journal of neuroinflammation 18:2 PubMed33402181

2021

Mer regulates microglial/macrophage M1/M2 polarization and alleviates neuroinflammation following traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Haijian Wu,Jingwei Zheng,Shenbin Xu,Yuanjian Fang,Yingxi Wu,Jianxiong Zeng,Anwen Shao,Ligen Shi,Jianan Lu,Shuhao Mei,Xiaoyu Wang,Xinying Guo,Yirong Wang,Zhen Zhao,Jianmin Zhang

Cell death and differentiation 28:1284-1300 PubMed33139930

2020

KPNB1-mediated nuclear translocation of PD-L1 promotes non-small cell lung cancer cell proliferation via the Gas6/MerTK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wenwen Du,Jianjie Zhu,Yuanyuan Zeng,Ting Liu,Yang Zhang,Tingting Cai,Yulong Fu,Weijie Zhang,Ruochen Zhang,Zeyi Liu,Jian-An Huang

PloS one 14:e0216275 PubMed31042769

2019

Csf1r or Mer inhibition delays liver regeneration via suppression of Kupffer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Juan A Santamaria-Barria,Shan Zeng,Jonathan B Greer,Michael J Beckman,Adrian M Seifert,Noah A Cohen,Jennifer Q Zhang,Megan H Crawley,Benjamin L Green,Jennifer K Loo,Joanna H Maltbaek,Ronald P DeMatteo

Arthritis & rheumatology (Hoboken, N.J.) 70:891-902 PubMed29342502

2018

Antiphospholipid Antibodies Inhibit Trophoblast Toll-Like Receptor and Inflammasome Negative Regulators.

Applications

Unspecified application

Species

Unspecified reactive species

Melissa J Mulla,Ingrid C Weel,Julie A Potter,Stefan M Gysler,Jane E Salmon,Maria T S Peraçoli,Carla V Rothlin,Lawrence W Chamley,Vikki M Abrahams

View all publications

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