AB215438

Anti-Mesothelin antibody

Name: Anti-Mesothelin antibody (ab215438) | Abcam
Brand: Abcam Limited
SKU: AB215438

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(1 Publication)

Rabbit Polyclonal Mesothelin antibody. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MSLN aa 100-200 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

MPF, MSLN, Mesothelin, CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody (AB215438)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody (AB215438)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling Mesothelin with ab215438 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Flow Cytometry (Intracellular) - Anti-Mesothelin antibody (AB215438)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Mesothelin antibody (AB215438)

Intracellular flow cytometric analysis of A549 cells labeling Mesothelin with ab215438 at 1/100 dilution for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Lab

Western blot - Anti-Mesothelin antibody (AB215438)

All lanes:

Western blot - Anti-Mesothelin antibody (ab215438) at 1/300 dilution

All lanes:

Hela lysates

Secondary

All lanes:

Conjugated secondary antibody at 1/10000 dilution

Predicted band size: 69 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Human MSLN aa 100-200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q13421

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/300", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20 - 1/100", "FlowCytIntra-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Membrane-anchored forms may play a role in cellular adhesion.. Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.

See full target information MSLN

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 44:112 PubMed40181456

2025

TRAF7 knockdown induces cellular senescence and synergizes with lomustine to inhibit glioma progression and recurrence.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Chen,Tongyu Zhou,Rongrong Zhou,Wen Sun,Yan Li,Qiyi Zhou,Dongcheng Xu,Yuxin Zhao,Peihao Hu,Jingrui Liang,Yumeng Zhang,Bin Zhong,Juncheng Yao,Di Jing

View all publications

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