AB187063

Anti-Mesothelin antibody [EPR17823-52]

RabMAb
Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal Mesothelin antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Mouse samples. Cited in 4 publications.

View Alternative Names

Mes, Mpf, Msln, Mesothelin, Pre-pro-megakaryocyte-potentiating factor

4 Images

Flow Cytometry (Intracellular) - Anti-Mesothelin antibody [EPR17823-52] (AB187063)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Mesothelin antibody [EPR17823-52] (AB187063)

Intracellular Flow Cytometry analysis of NIH/3T3 (mouse embryo fibroblast cell line) cells labeling Mesothelin with ab187063 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 was used as the secondary antibody (red). Rabbit monoclonal IgG (ab172730) was used as the isotype control (black). Blue - Unlabeled control, cells without incubation with primary and secondary antibodies.

Supplier Data

Immunoprecipitation - Anti-Mesothelin antibody [EPR17823-52] (AB187063)

Mesothelin was immunoprecipitated from 1 mg of NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate with ab187063 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab187063 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : NIH/3T3 whole cell lysate 10 μg (Input).

Lane 2 : ab187063 IP in NIH/3T3 whole cell lysate (+).

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab187063 in NIH/3T3 whole cell lysate (-).

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-Mesothelin antibody [EPR17823-52] (ab187063)

Predicted band size: 69 kDa

false

Supplier Data

Western blot - Anti-Mesothelin antibody [EPR17823-52] (AB187063)

Blocking/Dilution buffer : 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID : 16857795; PMID : 17332303).

All lanes:

Western blot - Anti-Mesothelin antibody [EPR17823-52] (ab187063) at 1/1000 dilution

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 10 µg

Lane 2:

Mouse lung lysate at 10 µg

Lane 3:

Mouse heart lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 69 kDa

Observed band size: 40 kDa,71 kDa

true

Exposure time: 3min

Supplier Data

Western blot - Anti-Mesothelin antibody [EPR17823-52] (AB187063)

Blocking/Dilution buffer : 5% NFDM/TBST.

Lane 1:

Western blot - Anti-Mesothelin antibody [EPR17823-52] (ab187063) at 1/1000 dilution

Lane 2:

Western blot - Anti-Mesothelin antibody [EPR17823-52] (ab187063) at 1/5000 dilution

Lane 3:

Western blot - Anti-Mesothelin antibody [EPR17823-52] (ab187063) at 1/20000 dilution

All lanes:

His-tagged mouse mesothelin fragment recombinant protein (aa298-600) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 69 kDa

Observed band size: 40 kDa

true

Carrier free

Anti-Mesothelin antibody [EPR17823-52] - BSA and Azide free
Carrier free

Anti-Mesothelin antibody [EPR17823-52] - BSA and Azide free (Capture)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17823-52

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

IP, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody recognizes a different epitope of Mesothelin compared to ab213174.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Membrane-anchored forms may play a role in cellular adhesion.. Megakaryocyte-potentiating factor (MPF) may potentiate megakaryocyte colony formation.

See full target information Msln

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Diabetology & metabolic syndrome 17:378 PubMed41044791

2025

Electroacupuncture ameliorates cognitive dysfunction in T2DM rats by modulating astrocytic polarization and aberrant energy metabolism in the hippocampus via the Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolu Li,Rui Li,Jiayi Lin,Xiaoli Wu

JCI insight 9: PubMed39106104

2024

Phosphodiesterase-5 inhibition collaborates with vaccine-based immunotherapy to reprogram myeloid cells in pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Nicole E Gross,Zhehao Zhang,Jacob T Mitchell,Soren Charmsaz,Alexei G Hernandez,Erin M Coyne,Sarah M Shin,Diana Carolina Vargas Carvajal,Dimitrios N Sidiropoulos,Yeonju Cho,Guanglan Mo,Xuan Yuan,Courtney Cannon,Jayalaxmi Suresh Babu,Melissa R Lyman,Todd Armstrong,Luciane T Kagohara,Katherine M Bever,Dung T Le,Elizabeth M Jaffee,Elana J Fertig,Won Jin Ho

Molecular therapy : the journal of the American Society of Gene Therapy 32:2984-2991 PubMed38414241

2024

Osr1-mediated mesothelial transition of liver mesenchymal cells exacerbates fibrotic liver damage.

Applications

Unspecified application

Species

Unspecified reactive species

Xinxin Nian,Pengyan Lin,Yunfei Bai,Donglin Yu,Xinyan Yang,Bin Zhou,Jie Gao,Yang Zhao

Journal for immunotherapy of cancer 12: PubMed38191243

2024

Clinically relevant orthotopic pancreatic cancer models for adoptive T cell transfer therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Natalie K Horvat,Isaac Karpovsky,Maggie Phillips,Megan M Wyatt,Margaret A Hall,Cameron J Herting,Jacklyn Hammons,Zaid Mahdi,Richard A Moffitt,Chrystal M Paulos,Gregory B Lesinski

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Mesothelin antibody [EPR17823-52]

Flow Cytometry (Intracellular) - Anti-Mesothelin antibody [EPR17823-52] (AB187063)

Immunoprecipitation - Anti-Mesothelin antibody [EPR17823-52] (AB187063)

Western blot - Anti-Mesothelin antibody [EPR17823-52] (AB187063)

Western blot - Anti-Mesothelin antibody [EPR17823-52] (AB187063)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

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