Rabbit Recombinant Monoclonal Mesothelin antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Mouse samples.
Images
![Immunoprecipitation - Anti-Mesothelin antibody [EPR17823-69] - BSA and Azide free (AB232219), expandable thumbnail](https://content.abcam.com/products/images/mesothelin-antibody-epr17823-69-bsa-and-azide-free-ab232219--immunoprecipitation-img776.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Mesothelin antibody [EPR17823-69] - BSA and Azide free (AB232219)")
![Western blot - Anti-Mesothelin antibody [EPR17823-69] - BSA and Azide free (AB232219), expandable thumbnail](https://content.abcam.com/products/images/mesothelin-antibody-epr17823-69-bsa-and-azide-free-ab232219--western-blot-img20157.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Mesothelin antibody [EPR17823-69] - BSA and Azide free (AB232219)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [EPR17823-69] - BSA and Azide free (AB232219), expandable thumbnail](https://content.abcam.com/products/images/mesothelin-antibody-epr17823-69-bsa-and-azide-free-ab232219--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img430424.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [EPR17823-69] - BSA and Azide free (AB232219)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [EPR17823-69] - BSA and Azide free (AB232219), expandable thumbnail](https://content.abcam.com/products/images/mesothelin-antibody-epr17823-69-bsa-and-azide-free-ab232219--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img430425.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [EPR17823-69] - BSA and Azide free (AB232219)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Mouse | Tested | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Membrane-anchored forms may play a role in cellular adhesion. Megakaryocyte-potentiating factor (MPF) may potentiate megakaryocyte colony formation.
Alternative names
Mes, Mpf, Msln, Mesothelin, Pre-pro-megakaryocyte-potentiating factor
Recommended products
Rabbit Recombinant Monoclonal Mesothelin antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR17823-69
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody recognizes a different epitope of Mesothelin compared to ab187063.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab232219 is the carrier-free version of Anti-Mesothelin antibody [EPR17823-69] ab213174.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunoprecipitation - Anti-Mesothelin antibody [EPR17823-69] - BSA and Azide free (ab232219), expandable thumbnail](https://content.abcam.com/products/images/mesothelin-antibody-epr17823-69-bsa-and-azide-free-ab232219--immunoprecipitation-img776.jpg "Immunoprecipitation - Anti-Mesothelin antibody [EPR17823-69] - BSA and Azide free (ab232219)")
Immunoprecipitation - Anti-Mesothelin antibody [EPR17823-69] - BSA and Azide free (ab232219)
Mesothelin was immunoprecipitated from 1 mg NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate with Anti-Mesothelin antibody [EPR17823-69] ab213174 at 1/40 dilution. Western blot was performed from the immunoprecipitate using Anti-Mesothelin antibody [EPR17823-69] ab213174 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: NIH/3T3 whole cell lysate 10 μg (Input).
Lane 2: Anti-Mesothelin antibody [EPR17823-69] ab213174 IP NIH/3T3 whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Mesothelin antibody [EPR17823-69] ab213174 in NIH/3T3 whole cell lysate (-)Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Mesothelin antibody [EPR17823-69] ab213174).
All lanes: Immunoprecipitation - Anti-Mesothelin antibody [EPR17823-69] (Anti-Mesothelin antibody [EPR17823-69] ab213174)
Predicted band size: 69 kDa
Observed band size: 40 kDa, 70 kDa
Exposure time: 3s
![Western blot - Anti-Mesothelin antibody [EPR17823-69] - BSA and Azide free (ab232219), expandable thumbnail](https://content.abcam.com/products/images/mesothelin-antibody-epr17823-69-bsa-and-azide-free-ab232219--western-blot-img20157.jpg "Western blot - Anti-Mesothelin antibody [EPR17823-69] - BSA and Azide free (ab232219)")
Western blot - Anti-Mesothelin antibody [EPR17823-69] - BSA and Azide free (ab232219)
Blocking and dilution buffer: 5% NFDM /TBST.
The expression profile observed is consistent with what has been described in the literature (PMID: 16857795; PMID: 17332303).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Mesothelin antibody [EPR17823-69] ab213174).
All lanes: Western blot - Anti-Mesothelin antibody [EPR17823-69] (Anti-Mesothelin antibody [EPR17823-69] ab213174) at 1/1000 dilution
Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 10 µg
Lane 2: Mouse lung lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 69 kDa
Observed band size: 40 kDa, 71 kDa
Exposure time: 3min
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [EPR17823-69] - BSA and Azide free (ab232219), expandable thumbnail](https://content.abcam.com/products/images/mesothelin-antibody-epr17823-69-bsa-and-azide-free-ab232219--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img430424.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [EPR17823-69] - BSA and Azide free (ab232219)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [EPR17823-69] - BSA and Azide free (ab232219)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Mesothelin antibody [EPR17823-69] ab213174).
Immunohistochemical analysis of paraffin-embedded mouse lung labelling Mesothelin with Anti-Mesothelin antibody [EPR17823-69] ab213174 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Positive staining on mesothelium of mouse lung.
The section was incubated with Anti-Mesothelin antibody [EPR17823-69] ab213174 at 4°C overnight.
Counterstained with Hematoxylin.
Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [EPR17823-69] - BSA and Azide free (ab232219), expandable thumbnail](https://content.abcam.com/products/images/mesothelin-antibody-epr17823-69-bsa-and-azide-free-ab232219--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img430425.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [EPR17823-69] - BSA and Azide free (ab232219)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [EPR17823-69] - BSA and Azide free (ab232219)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Mesothelin antibody [EPR17823-69] ab213174).
Immunohistochemical analysis of paraffin-embedded mouse spleen labelling Mesothelin with Anti-Mesothelin antibody [EPR17823-69] ab213174 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Positive staining on mesothelium of mouse spleen.
The section was incubated with Anti-Mesothelin antibody [EPR17823-69] ab213174 at 4°C overnight.
Counterstained with Hematoxylin.
Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com