Anti-Mesothelin antibody [EPR2685(2)] (ab134109)

Rabbit Recombinant Monoclonal Mesothelin antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication.

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Images

Immunoprecipitation - Anti-Mesothelin antibody [EPR2685(2)] (AB134109), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	IP	WB	ICC/IF
Human	Tested	Tested	Tested	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500 - 1/2000	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/10 - 1/100	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000 - 1/10000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Associated Products

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Target data

See full target information MSLN

Function

Membrane-anchored forms may play a role in cellular adhesion. Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.

Alternative names

MPF, MSLN, Mesothelin, CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor

Recommended products

Rabbit Recombinant Monoclonal Mesothelin antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR2685(2)
Purification technique: Affinity purification Protein A
Dissociation constant: 5.5 x 10^-12 M
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Stable for 12 months at -20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

12 product images

Immunoprecipitation - Anti-Mesothelin antibody [EPR2685(2)] (ab134109)
ab134109 (purified) at 1/40 immunoprecipitating mesothelin in 10 μg HeLa cell lysate (Lanes 1 and 2, observed at 40 and 69 kDa). Lane 3 - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
All lanes: Immunoprecipitation - Anti-Mesothelin antibody [EPR2685(2)] (ab134109)
Predicted band size: 69 kDa
Western blot - Anti-Mesothelin antibody [EPR2685(2)] (ab134109)
False colour image of Western blot: Anti-Mesothelin antibody [EPR2685(2)] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab134109 was shown to bind specifically to Mesothelin. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
All lanes: Western blot - Anti-Mesothelin antibody [EPR2685(2)] (ab134109) at 1/1000 dilution
Lane 1: OVCAR-3 cell lysate at 20 µg
Lane 2: HeLa cell lysate at 20 µg
Lane 3: A549 cell lysate at 20 µg
Lane 4: PC-3 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 45 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [EPR2685(2)] (ab134109)
Immunohistochemical staining of paraffin embedded human skeletal muscle with purified ab134109 at a working dilution of 1/800. The secondary antibody used is Goat Anti-Rabbit IgG H&L (HRP) ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Western blot - Anti-Mesothelin antibody [EPR2685(2)] (ab134109)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Mesothelin antibody [EPR2685(2)] (ab134109) at 1/1000 dilution
All lanes: HeLa whole cell lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 42 kDa, 46 kDa, 69 kDa, 79 kDa
Observed band size: 40 kDa, 42 kDa, 69 kDa, 79 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [EPR2685(2)] (ab134109)
Immunohistochemical staining of paraffin embedded human mesothelioma with purified ab134109 at a working dilution of 1/800. The secondary antibody used is Goat Anti-Rabbit IgG H&L (HRP) ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Western blot - Anti-Mesothelin antibody [EPR2685(2)] (ab134109)
All lanes: Western blot - Anti-Mesothelin antibody [EPR2685(2)] (ab134109) at 1/1000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: Human fetal lung lysate at 10 µg
Secondary
All lanes: Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 17 kDa, 19 kDa, 36 kDa, 69 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [EPR2685(2)] (ab134109)
Immunohistochemical analysis of paraffin embedded Human mesothelioma tissue labelling Mesothelin with unpurified ab134109 at 1/1000.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [EPR2685(2)] (ab134109)
Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue labelling Mesothelin with unpurified ab134109 at 1/1000.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [EPR2685(2)] (ab134109)
Immunohistochemical analysis of paraffin embedded normal Human pancreas tissue using unpurified ab134109 showing -ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [EPR2685(2)] (ab134109)
Immunohistochemical analysis of paraffin embedded Human Pancreatic carcinoma tissue using unpurified ab134109 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [EPR2685(2)] (ab134109)
Immunohistochemical analysis of paraffin embedded Human Greator omentum tissue using unpurified ab134109 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
OI-RD Scanning - Anti-Mesothelin antibody [EPR2685(2)] (ab134109)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.