AB133489

Anti-Mesothelin antibody [EPR4509]

RabMAb
Recombinant
What is this?

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(8 Publications)

Rabbit Recombinant Monoclonal Mesothelin antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 8 publications.

View Alternative Names

MPF, MSLN, Mesothelin, CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [EPR4509] (AB133489)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [EPR4509] (AB133489)

Immunohistochemical analysis of Mesothelin in paraffin embedded Human serous ovarian carcinoma tissue using ab133489 at a dilution of 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Lab

Immunoprecipitation - Anti-Mesothelin antibody [EPR4509] (AB133489)

Purified ab133489 at 1/40 dilution (2μg) immunoprecipitating Mesothelin in HeLa whole cell lysate.
Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg
Lane 2 (+) : ab133489 + HeLa whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab133489 in HeLa whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 40, 71 kDa
The expression profile observed is consistent with what has been described in the literature (PMID : 16857795; PMID : 17332303).

All lanes:

Immunoprecipitation - Anti-Mesothelin antibody [EPR4509] (ab133489)

Predicted band size: 69 kDa

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Western blot - Anti-Mesothelin antibody [EPR4509] (AB133489)

All lanes:

Western blot - Anti-Mesothelin antibody [EPR4509] (ab133489) at 1/1000 dilution

Lane 1:

Human fetal lung lysate at 10 µg

Lane 2:

Human breast cancer lysate at 10 µg

Lane 3:

HeLa lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 69 kDa

false

Carrier free

Anti-Mesothelin antibody [EPR4509] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4509

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Membrane-anchored forms may play a role in cellular adhesion.. Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.

See full target information MSLN

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:7102 PubMed40016251

2025

Development of a novel postoperative adhesion induction model in cynomolgus monkeys with high reliability and reproducibility.

Applications

Unspecified application

Species

Unspecified reactive species

Kenji Nakagawa,Masaki Yamazaki,Hiromi Tanimura,Narumi Sakaguchi,Miho Kohara,Izumi Sato,Masahiro Azuma,Ayako Nishimoto-Kakiuchi,Atsuhiko Kato,Takehisa Kitazawa,Ryo Konno,Tadashi Sankai

Journal for immunotherapy of cancer 12: PubMed39433427

2024

Microenvironmental alkalization promotes the therapeutic effects of MSLN-CAR-T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Min Wu,Ling Mao,Xuejia Zhai,Jie Liu,Junhan Wang,Langhong Li,Jiangjie Duan,Jun Wang,Shuang Lin,Jianjun Li,Shicang Yu

Journal of experimental & clinical cancer research : CR 43:103 PubMed38570866

2024

Mesothelin promotes brain metastasis of non-small cell lung cancer by activating MET.

Applications

Unspecified application

Species

Unspecified reactive species

Shengkai Xia,Wenzhe Duan,Mingxin Xu,Mengqi Li,Mengyi Tang,Song Wei,Manqing Lin,Encheng Li,Wenwen Liu,Qi Wang

Frontiers in oncology 12:830570 PubMed35692779

2022

MSLN Correlates With Immune Infiltration and Chemoresistance as a Prognostic Biomarker in Ovarian Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yike Li,Wanjia Tian,Hong Zhang,Zhijian Zhang,Qinghe Zhao,Lei Chang,Ningjing Lei,Weiwei Zhang

Molecular therapy oncolytics 16:262-271 PubMed32181327

2020

Inhibition of Cholesterol Esterification Enzyme Enhances the Potency of Human Chimeric Antigen Receptor T Cells against Pancreatic Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Zhao,Yang Liu,Fuya Zhao,Ye Jin,Jing Feng,Rui Geng,Jiayu Sun,Liqing Kang,Lei Yu,Yunwei Wei

International journal of medical sciences 17:422-427 PubMed32174772

2020

Overexpression of Mesothelin in Pancreatic Ductal Adenocarcinoma (PDAC).

Applications

Unspecified application

Species

Unspecified reactive species

Kai Le,Jia Wang,Tao Zhang,Yifan Guo,Hong Chang,Siyuan Wang,Bin Zhu

OncoTargets and therapy 12:10043-10055 PubMed31819500

2019

Prevalence of DLL3, CTLA-4 and MSTN Expression in Patients with Small Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Orgilmaa Regzedmaa,Ying Li,Yongwen Li,Hongbing Zhang,Jin Wang,Hao Gong,Yin Yuan,Weiting Li,Hongyu Liu,Jun Chen

Canadian journal of kidney health and disease 4:2054358117699829 PubMed28540060

2017

Microparticle Formation in Peritoneal Dialysis: A Proof of Concept Study.

Applications

Unspecified application

Species

Unspecified reactive species

Shareef Akbari,Rima Abou-Arkoub,Suzy Sun,Swapnil Hiremath,Arkadiy Reunov,Brendan B McCormick,Marcel Ruzicka,Dylan Burger

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Mesothelin antibody [EPR4509]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [EPR4509] (AB133489)

Immunoprecipitation - Anti-Mesothelin antibody [EPR4509] (AB133489)

Western blot - Anti-Mesothelin antibody [EPR4509] (AB133489)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (8)

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