Anti-Mesothelin antibody [MSLN/2131] - BSA and Azide free (ab237817)

Mouse Monoclonal Mesothelin antibody. Carrier free. Suitable for Protein Array, IHC-P and reacts with Recombinant full length protein - Human, Mouse, Rat, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MSLN aa 250-450.

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Protein Array - Anti-Mesothelin antibody [MSLN/2131] - BSA and Azide free (AB237817), expandable thumbnail

Key facts

Isotype: IgG2b
Host species: Mouse
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

Recombinant Fragment Protein within Human MSLN aa 250-450. The exact immunogen used to generate this antibody is proprietary information. Database link Q13421

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	Protein Array	IHC-P
Human	Expected	Tested
Mouse	Expected	Tested
Rat	Expected	Tested
Recombinant full length protein - Human	Tested	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Recombinant full length protein - Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1-2 µg/mL	Notes Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Incubate with primary antibody for 30 minutes at RT.
Species Rat	Dilution info 1-2 µg/mL	Notes Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Incubate with primary antibody for 30 minutes at RT.
Species Human	Dilution info 1-2 µg/mL	Notes Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Incubate with primary antibody for 30 minutes at RT.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant full length protein - Human	Dilution info -	Notes -

Associated Products

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1 product for Alternative Version

7 products for Alternative Product

Target data

See full target information MSLN

Function

Membrane-anchored forms may play a role in cellular adhesion. Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.

Alternative names

MPF, MSLN, Mesothelin, CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor

Key facts

Isotype: IgG2b
Host species: Mouse
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: Recombinant Fragment Protein within Human MSLN aa 250-450. The exact immunogen used to generate this antibody is proprietary information. Database link Q13421
Carrier free: Yes
Clone number: MSLN/2131
Purification technique: Affinity purification Protein A/G
Light chain type: kappa
Concentration
Purification notes: Purified from bioreactor concentrate by Protein A/G.

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Product promise

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6 product images

Protein Array - Anti-Mesothelin antibody [MSLN/2131] - BSA and Azide free (ab237817)
This data was produced with Anti-Mesothelin antibody [MSLN/2131] ab236546, the same antibody in a different formulation with BSA and Azide.
Anti-Mesothelin antibody [MSLN/2131] ab236546 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [MSLN/2131] - BSA and Azide free (ab237817)
Formalin-fixed, paraffin-embedded rat stomach tissue stained for Mesothelin with ab237817 at 2 μg/ml in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [MSLN/2131] - BSA and Azide free (ab237817)
Formalin-fixed, paraffin-embedded mouse lung tissue stained for Mesothelin with ab237817 at 2 μg/ml in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [MSLN/2131] - BSA and Azide free (ab237817)
Formalin-fixed, paraffin-embedded human tonsil tissue stained for Mesothelin with ab237817 at 2 μg/ml in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [MSLN/2131] - BSA and Azide free (ab237817)
Formalin-fixed, paraffin-embedded human endometrial carcinoma tissue stained for Mesothelin with ab237817 at 2 μg/ml in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [MSLN/2131] - BSA and Azide free (ab237817)
Formalin-fixed, paraffin-embedded human lung mesothelioma tissue stained for Mesothelin with ab237817 at 2 μg/ml in immunohistochemical analysis.

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Anti-Mesothelin antibody [MSLN/2131] - BSA and Azide free

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