Rabbit Recombinant Monoclonal Mesothelin antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications.
Images
![Western blot - Anti-Mesothelin antibody [SP74] (AB93620), expandable thumbnail](https://content.abcam.com/products/images/mesothelin-antibody-sp74-ab93620--western-blot-img20891.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Mesothelin antibody [SP74] (AB93620)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [SP74] (AB93620), expandable thumbnail](https://content.abcam.com/products/images/mesothelin-antibody-sp74-ab93620--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img40420.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [SP74] (AB93620)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [SP74] (AB93620), expandable thumbnail](https://content.abcam.com/products/images/mesothelin-antibody-sp74-ab93620--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img82823.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [SP74] (AB93620)")
Publications
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- British journal of cancer 126:754-7632021Exploiting mesothelin in thymic carcinoma as a drug delivery target for anetumab ravtansine.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVincent Chen,Shigeki Umemura,Yumin Han,Renuka Raman,Robin Tucker,Joeffrey Chahine,In-Kyu Kim,Christoph Schatz,Sabine Zitzmann-Kolbe,Anette Sommer,Masanori Onda,Trevor Lee,Yongfeng He,Giuseppe GiacconePubMed 34876673
- Medicine 98:e162072019Mesothelin expression and its prognostic role according to microsatellite instability status in colorectal adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyunsung Kim,Yumin Chung,Seung Sam Paik,Kiseok Jang,Su-Jin ShinPubMed 31261569
- The Journal of clinical investigation 127:2176-21912017Biopolymers codelivering engineered T cells and STING agonists can eliminate heterogeneous tumors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTyrel T Smith,Howell F Moffett,Sirkka B Stephan,Cary F Opel,Amy G Dumigan,Xiuyun Jiang,Venu G Pillarisetty,Smitha P S Pillai,K Dane Wittrup,Matthias T StephanPubMed 28436934
- International journal of clinical and experimental 6:2476-862013Diagnostic and therapeutic implications of a novel immunohistochemical panel detecting duodenal mucosal invasion by pancreatic ductal adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSabrina C Sopha et. al.PubMed 24228110
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Pig | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.322 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
Associated Products
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Target data
Function
Membrane-anchored forms may play a role in cellular adhesion. Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.
Alternative names
MPF, MSLN, Mesothelin, CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor
Recommended products
Rabbit Recombinant Monoclonal Mesothelin antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- SP74
- Purification technique
- Affinity purification Protein A/G
- Concentration
- Purification notes
Purified from TCS by protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Mesothelin antibody [SP74] (ab93620), expandable thumbnail](https://content.abcam.com/products/images/mesothelin-antibody-sp74-ab93620--western-blot-img20891.jpg "Western blot - Anti-Mesothelin antibody [SP74] (ab93620)")
Western blot - Anti-Mesothelin antibody [SP74] (ab93620)
Blocking/Diluting buffer and concentration: 5% NFDM /TBST
All lanes: Western blot - Anti-Mesothelin antibody [SP74] (ab93620) at 0.322 µg/mL
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) cell lysate at 20 µg
Lane 2: BxPC-3 (Human pancreas adenocarcinoma epithelial cell) cell lysate at 20 µg
Lane 3: PC3 (Human prostate adenocarcinoma epithelial cell) cell lysate at 20 µg
Lane 4: A549 (Human lung carcinoma epithelial cell) cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 0.05 µg/mL
Predicted band size: 69 kDa
Observed band size: 49 kDa, 69 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [SP74] (ab93620), expandable thumbnail](https://content.abcam.com/products/images/mesothelin-antibody-sp74-ab93620--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img40420.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [SP74] (ab93620)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [SP74] (ab93620)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human mesothelioma tissue sections labeling Mesothelin with Purified ab93620 at 1/100 dilution (1.4 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [SP74] (ab93620), expandable thumbnail](https://content.abcam.com/products/images/mesothelin-antibody-sp74-ab93620--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img82823.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [SP74] (ab93620)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [SP74] (ab93620)
Human Mesothelioma stained with Anti-Mesothelin antibody [SP74] (ab93620)
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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