AB93620

Anti-Mesothelin antibody [SP74]

Name: Anti-Mesothelin antibody [SP74] Rabbit monoclonal (ab93620) | Abcam
Brand: Abcam Limited
SKU: AB93620
Price: 210 USD
Availability: InStock
Rating: 5 (2 reviews)

RabMAb
Recombinant
What is this?

(2 Reviews)

(6 Publications)

Rabbit Recombinant Monoclonal Mesothelin antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 6 publications.

View Alternative Names

MPF, MSLN, Mesothelin, CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [SP74] (AB93620)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [SP74] (AB93620)

Human Mesothelioma stained with Anti-Mesothelin antibody [SP74] (ab93620)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [SP74] (AB93620)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human mesothelioma tissue sections labeling Mesothelin with Purified ab93620 at 1/100 dilution (1.4 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Lab

Western blot - Anti-Mesothelin antibody [SP74] (AB93620)

Blocking/Diluting buffer and concentration : 5% NFDM /TBST

All lanes:

Western blot - Anti-Mesothelin antibody [SP74] (ab93620) at 0.322 µg/mL

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) cell lysate at 20 µg

Lane 2:

BxPC-3 (Human pancreas adenocarcinoma epithelial cell) cell lysate at 20 µg

Lane 3:

PC3 (Human prostate adenocarcinoma epithelial cell) cell lysate at 20 µg

Lane 4:

A549 (Human lung carcinoma epithelial cell) cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 0.05 µg/mL

Predicted band size: 69 kDa

Observed band size: 49 kDa,69 kDa

false

603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Mesothelin antibody [SP74]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Mesothelin antibody [SP74]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Mesothelin antibody [SP74]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Mesothelin antibody [SP74]
Carrier free

Anti-Mesothelin antibody [SP74] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Mesothelin antibody [SP74]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Mesothelin antibody [SP74]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP74

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.322 µg/mL", "WB-species-notes": "<p></p>" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from TCS by protein A/G.

Storage buffer

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Membrane-anchored forms may play a role in cellular adhesion.. Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.

See full target information MSLN

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:5902 PubMed40593704

2025

Generation of mature epicardium derived from human-induced pluripotent stem cells via inhibition of mTOR signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Tian,Antonio Lucena-Cacace,Kanae Tani,Amanda Putri Elvandari,Rodolfo S Allendes Osorio,Megumi Narita,Yasuko Matsumura,Ian Costa Paixao,Yutaro Miyoshi,Azusa Inagaki,Julia Junghof,Yoshinori Yoshida

Cancers 15: PubMed37345150

2023

Genomic and Transcriptomic Analyses of Malignant Pleural Mesothelioma (MPM) Samples Reveal Crucial Insights for Preclinical Testing.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander Laure,Angelica Rigutto,Michaela B Kirschner,Lennart Opitz,Linda Grob,Isabelle Opitz,Emanuela Felley-Bosco,Stefanie Hiltbrunner,Alessandra Curioni-Fontecedro

British journal of cancer 126:754-763 PubMed34876673

2021

Exploiting mesothelin in thymic carcinoma as a drug delivery target for anetumab ravtansine.

Applications

Unspecified application

Species

Unspecified reactive species

Vincent Chen,Shigeki Umemura,Yumin Han,Renuka Raman,Robin Tucker,Joeffrey Chahine,In-Kyu Kim,Christoph Schatz,Sabine Zitzmann-Kolbe,Anette Sommer,Masanori Onda,Trevor Lee,Yongfeng He,Giuseppe Giaccone

Medicine 98:e16207 PubMed31261569

2019

Mesothelin expression and its prognostic role according to microsatellite instability status in colorectal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hyunsung Kim,Yumin Chung,Seung Sam Paik,Kiseok Jang,Su-Jin Shin

The Journal of clinical investigation 127:2176-2191 PubMed28436934

2017

Biopolymers codelivering engineered T cells and STING agonists can eliminate heterogeneous tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Tyrel T Smith,Howell F Moffett,Sirkka B Stephan,Cary F Opel,Amy G Dumigan,Xiuyun Jiang,Venu G Pillarisetty,Smitha P S Pillai,K Dane Wittrup,Matthias T Stephan

International journal of clinical and experimental 6:2476-86 PubMed24228110

2013

Diagnostic and therapeutic implications of a novel immunohistochemical panel detecting duodenal mucosal invasion by pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Sabrina C Sopha,Purva Gopal,Nipun B Merchant,Frank L Revetta,David V Gold,Kay Washington,Chanjuan Shi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Mesothelin antibody [SP74]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [SP74] (AB93620)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [SP74] (AB93620)

Western blot - Anti-Mesothelin antibody [SP74] (AB93620)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (6)

Product promise