AB151564

Anti-MEST antibody [EPR6970(2)]

RabMAb
Recombinant
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(6 Publications)

Rabbit Recombinant Monoclonal MEST antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications.

View Alternative Names

PEG1, MEST, Mesoderm-specific transcript homolog protein, Paternally-expressed gene 1 protein

1 Images

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Western blot - Anti-MEST antibody [EPR6970(2)] (AB151564)

All lanes:

Western blot - Anti-MEST antibody [EPR6970(2)] (ab151564) at 1/1000 dilution

Lane 1:

Human placenta lysate at 10 µg

Lane 2:

Human fetal kidney lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 38 kDa

false

Carrier free

Anti-MEST antibody [EPR6970(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6970(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 'MEST' protein also often referred to as the 'MEST band' is an imprinted gene product with molecular mass approximately 40 kDa. It displays biallelic and tissue-specific expression being especially present in tissues like placenta fetal tissues and adipose tissue. MEST also plays a critical role in development and metabolism providing an essential regulatory function in these tissues. The protein's expression levels can vary due to genomic imprinting which leads to selective expression from the paternal allele in certain contexts.

Biological function summary

The MEST protein participates in key cellular processes such as embryonic growth and adipocyte metabolism. It has a role in fetal development influencing growth rates and differentiation in tissues where it is expressed. If included in complex formations MEST may interact with other proteins to modulate signals that are important in these developmental processes. Given its involvement in adipogenesis MEST is also significant in energy homeostasis and fat storage impacting the body's metabolic responses.

Pathways

The MEST protein integrates into the adipogenesis signaling pathway contributing to the formation and function of adipose tissue. Additionally MEST is implicated in the insulin signaling pathway linking it to glucose metabolism and insulin sensitivity. Through these pathways MEST engages with other proteins such as PPARγ (Peroxisome proliferator-activated receptor gamma) and CEBPα (CCAAT/enhancer-binding protein alpha) which further highlights its involvement in regulating adipocyte differentiation and metabolic functions.

Alterations in MEST expression levels associate with various metabolic syndromes such as obesity and type 2 diabetes. These conditions reveal how improper regulation of MEST can disrupt energy balance and glucose metabolism. Its link to PPARγ highlights a connection to lipid disorders as this protein is important for lipid metabolism and any dysregulation might contribute to the development of metabolic-related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information MEST

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of ovarian research 18:151 PubMed40652270

2025

BFGF mitigates CTX-induced ovarian cytotoxicity via SERPINE1/HIF-1 and Nrf-2/HO-1 signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Li,Lin Zhang,Hongqing Lv,Chenxuan Mei,Min Hu

NPJ Regenerative medicine 9:32 PubMed39468052

2024

Regeneration-specific promoter switching facilitates Mest expression in the mouse digit tip to modulate neutrophil response.

Applications

Unspecified application

Species

Unspecified reactive species

Vivian Jou,Sophia M Peña,Jessica A Lehoczky

PloS one 17:e0271913 PubMed35867696

2022

Social and maternal behavior in mesoderm specific transcript (Mest)-deficient mice.

Applications

Unspecified application

Species

Unspecified reactive species

Rea P Anunciado-Koza,J Patrizia Stohn,Arturo Hernandez,Robert A Koza

Scientific reports 11:20075 PubMed34625606

2021

Hypermethylation of Mest promoter causes aberrant Wnt signaling in patients with Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Renuka Prasad,Hwajin Jung,Anderson Tan,Yonghee Song,Sungho Moon,Mohammed R Shaker,Woong Sun,Junghee Lee,Hoon Ryu,Hyun Kook Lim,Eek-Hoon Jho

Journal of experimental & clinical cancer research : CR 40:301 PubMed34560900

2021

MEST promotes lung cancer invasion and metastasis by interacting with VCP to activate NF-κB signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Wang,Jing Zhang,Yang-Jia Li,Nan-Nan Yu,Wan-Ting Liu,Jun-Ze Liang,Wen Wen Xu,Zheng-Hua Sun,Bin Li,Qing-Yu He

Development (Cambridge, England) 143:950-61 PubMed26893351

2016

The transcription factor SOX6 contributes to the developmental origins of obesity by promoting adipogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Shi Chi Leow,Jeremie Poschmann,Peh Gek Too,Juan Yin,Roy Joseph,Craig McFarlane,Shaillay Dogra,Asim Shabbir,Philip W Ingham,Shyam Prabhakar,Melvin K S Leow,Yung Seng Lee,Kai Lyn Ng,Yap Seng Chong,Peter D Gluckman,Walter Stünkel

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MEST antibody [EPR6970(2)]

Western blot - Anti-MEST antibody [EPR6970(2)] (AB151564)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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Product promise