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AB249008

Anti-MEST antibody [EPR6970(2)] - BSA and Azide free

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Rabbit Recombinant Monoclonal MEST antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

PEG1, MEST, Mesoderm-specific transcript homolog protein, Paternally-expressed gene 1 protein

1 Images
Western blot - Anti-MEST antibody [EPR6970(2)] - BSA and Azide free (AB249008)
  • WB

Unknown

Western blot - Anti-MEST antibody [EPR6970(2)] - BSA and Azide free (AB249008)

This data was developed using ab151564, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MEST antibody [EPR6970(2)] (<a href='/en-us/products/primary-antibodies/mest-antibody-epr69702-ab151564'>ab151564</a>) at 1/1000 dilution

Lane 1:

Human placenta lysate at 10 µg

Lane 2:

Human fetal kidney lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 38 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6970(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab249008 is the carrier-free version of ab151564.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 'MEST' protein also often referred to as the 'MEST band' is an imprinted gene product with molecular mass approximately 40 kDa. It displays biallelic and tissue-specific expression being especially present in tissues like placenta fetal tissues and adipose tissue. MEST also plays a critical role in development and metabolism providing an essential regulatory function in these tissues. The protein's expression levels can vary due to genomic imprinting which leads to selective expression from the paternal allele in certain contexts.
Biological function summary

The MEST protein participates in key cellular processes such as embryonic growth and adipocyte metabolism. It has a role in fetal development influencing growth rates and differentiation in tissues where it is expressed. If included in complex formations MEST may interact with other proteins to modulate signals that are important in these developmental processes. Given its involvement in adipogenesis MEST is also significant in energy homeostasis and fat storage impacting the body's metabolic responses.

Pathways

The MEST protein integrates into the adipogenesis signaling pathway contributing to the formation and function of adipose tissue. Additionally MEST is implicated in the insulin signaling pathway linking it to glucose metabolism and insulin sensitivity. Through these pathways MEST engages with other proteins such as PPARγ (Peroxisome proliferator-activated receptor gamma) and CEBPα (CCAAT/enhancer-binding protein alpha) which further highlights its involvement in regulating adipocyte differentiation and metabolic functions.

Alterations in MEST expression levels associate with various metabolic syndromes such as obesity and type 2 diabetes. These conditions reveal how improper regulation of MEST can disrupt energy balance and glucose metabolism. Its link to PPARγ highlights a connection to lipid disorders as this protein is important for lipid metabolism and any dysregulation might contribute to the development of metabolic-related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information MEST
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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