Rabbit Recombinant Monoclonal Met (c-Met) antibody. Carrier free. Suitable for IHC-P and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [CAL12] - BSA and Azide free (AB251598), expandable thumbnail](https://content.abcam.com/products/images/met-c-met-antibody-cal12-bsa-and-azide-free-ab251598--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img70035.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [CAL12] - BSA and Azide free (AB251598)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [CAL12] - BSA and Azide free (AB251598), expandable thumbnail](https://content.abcam.com/products/images/met-c-met-antibody-cal12-bsa-and-azide-free-ab251598--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img122834.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [CAL12] - BSA and Azide free (AB251598)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [CAL12] - BSA and Azide free (AB251598), expandable thumbnail](https://content.abcam.com/products/images/met-c-met-antibody-cal12-bsa-and-azide-free-ab251598--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img32537.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [CAL12] - BSA and Azide free (AB251598)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
3 products for Alternative Product
1 products for Alternative Version
Target data
Function
Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells. May regulate cortical bone osteogenesis (By similarity).(Microbial infection) Acts as a receptor for Listeria monocytogenes internalin InlB, mediating entry of the pathogen into cells.
Alternative names
Hepatocyte growth factor receptor, HGF receptor, HGF/SF receptor, Proto-oncogene c-Met, Scatter factor receptor, Tyrosine-protein kinase Met, SF receptor, MET
Recommended products
Rabbit Recombinant Monoclonal Met (c-Met) antibody. Carrier free. Suitable for IHC-P and reacts with Human samples.
Alternative names
Hepatocyte growth factor receptor, HGF receptor, HGF/SF receptor, Proto-oncogene c-Met, Scatter factor receptor, Tyrosine-protein kinase Met, SF receptor, MET
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
CAL12
- Purification technique
Affinity purification Protein A
- Concentration
- Purification notes
Purity is greater than 99%.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab251598 is the carrier-free version of Anti-Met (c-Met) antibody [CAL12] ab237711.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [CAL12] - BSA and Azide free (ab251598), expandable thumbnail](https://content.abcam.com/products/images/met-c-met-antibody-cal12-bsa-and-azide-free-ab251598--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img70035.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [CAL12] - BSA and Azide free (ab251598)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [CAL12] - BSA and Azide free (ab251598)
Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling Met (c-Met) with Anti-Met (c-Met) antibody [CAL12] ab237711 at 1:1000 dilution (0.45 μg/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on the human lung carcinoma.
The section was incubated with Anti-Met (c-Met) antibody [CAL12] ab237711 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Met (c-Met) antibody [CAL12] ab237711).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [CAL12] - BSA and Azide free (ab251598), expandable thumbnail](https://content.abcam.com/products/images/met-c-met-antibody-cal12-bsa-and-azide-free-ab251598--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img122834.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [CAL12] - BSA and Azide free (ab251598)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [CAL12] - BSA and Azide free (ab251598)
Formalin-fixed, paraffin-embedded human non-small cell lung carcinoma tissue stained for Met (c-Met) using Anti-Met (c-Met) antibody [CAL12] ab237711 at 1 μg/ml in immunohistochemical analysis.
Incubate with primary antibody for 75 minutes at room temperature.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Met (c-Met) antibody [CAL12] ab237711).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [CAL12] - BSA and Azide free (ab251598), expandable thumbnail](https://content.abcam.com/products/images/met-c-met-antibody-cal12-bsa-and-azide-free-ab251598--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img32537.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [CAL12] - BSA and Azide free (ab251598)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [CAL12] - BSA and Azide free (ab251598)
Formalin-fixed, paraffin-embedded human non-small cell lung carcinoma tissue stained for Met (c-Met) using Anti-Met (c-Met) antibody [CAL12] ab237711 at 1 μg/ml in immunohistochemical analysis.
Incubate with primary antibody for 75 minutes at room temperature.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Met (c-Met) antibody [CAL12] ab237711).
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