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Rabbit Recombinant Monoclonal Met (c-Met) antibody. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Mouse samples. Cited in 3 publications.

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Images

Immunoprecipitation - Anti-Met (c-Met) antibody [EPR22436-24] (AB216330), expandable thumbnail
  • Flow Cytometry - Anti-Met (c-Met) antibody [EPR22436-24] (AB216330), expandable thumbnail
  • Western blot - Anti-Met (c-Met) antibody [EPR22436-24] (AB216330), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Met (c-Met) antibody [EPR22436-24] (AB216330), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IF
Mouse
Tested
Tested
Tested
Not recommended
Tested

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of neuronal precursors, angiogenesis and kidney formation. During skeletal muscle development, it is crucial for the migration of muscle progenitor cells and for the proliferation of secondary myoblasts (PubMed:30777867). In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells (By similarity). May regulate cortical bone osteogenesis (PubMed:26637977). (Microbial infection) Acts as a receptor for Listeria monocytogenes internalin InlB, mediating entry of the pathogen into cells.

Alternative names

Hepatocyte growth factor receptor, HGF receptor, HGF/SF receptor, Proto-oncogene c-Met, Scatter factor receptor, Tyrosine-protein kinase Met, SF receptor, Met

Recommended products

Rabbit Recombinant Monoclonal Met (c-Met) antibody. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Mouse samples. Cited in 3 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22436-24
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunoprecipitation - Anti-Met (c-Met) antibody [EPR22436-24] (ab216330), expandable thumbnail

    Immunoprecipitation - Anti-Met (c-Met) antibody [EPR22436-24] (ab216330)

    Met (c-Met) was immunoprecipitated from 0.35mg of B16-F10 (Mouse melanoma mixture of spindle-shaped and epithelial-like cells) whole cell lysate using ab216330 at 1/30 dilution. WB was performed on the immunoprecipitate using ab216330 at 1/1000 dilution, followed by the VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution.

    Lane 1: B16-F10 whole cell lysate 10μg (input).
    Lane 2: ab216330 IP in B16-F10 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab216330 in B16-F10 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 seconds.

    All lanes: Immunoprecipitation - Anti-Met (c-Met) antibody [EPR22436-24] (ab216330)

    Predicted band size: 155 kDa

  • Flow Cytometry - Anti-Met (c-Met) antibody [EPR22436-24] (ab216330), expandable thumbnail

    Flow Cytometry - Anti-Met (c-Met) antibody [EPR22436-24] (ab216330)

    Flow cytometric analysis of 4% paraformaldehyde-fixed NIH/3T3 (Mouse embryonic fibroblast, Left) / B16-F10 (Mouse melanoma mixture of spindle-shaped and epithelial-like cells, Right) labeling Met (c-Met) with ab216330 at 1/500 dilution (red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody, Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/2000 dilution.

    Negative control: NIH/3T3(PMID: 21496277, 8197126, 9888438).

    Gated on viable cells.

  • Western blot - Anti-Met (c-Met) antibody [EPR22436-24] (ab216330), expandable thumbnail

    Western blot - Anti-Met (c-Met) antibody [EPR22436-24] (ab216330)

    Blocking/diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 26 seconds.

    Negative control: NIH/3T3 (PMID: 21496277; PMID: 8197126; PMID 9888438)

    The molecular weight observed is consistent with what has been described in the literature (PMID: 8710887).

    All lanes: Western blot - Anti-Met (c-Met) antibody [EPR22436-24] (ab216330) at 1/1000 dilution

    Lane 1: B16-F10 (mouse melanoma mixture of spindle-shaped and epithelial-like cells) whole cell lysate at 20 µg

    Lane 2: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 155 kDa

    Observed band size: 175 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Met (c-Met) antibody [EPR22436-24] (ab216330), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Met (c-Met) antibody [EPR22436-24] (ab216330)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized B16-F10 (mouse melanoma mixture of spindle-shaped and epithelial-like cells) and NIH/3T3 (mouse embryonic fibroblast) cells labelling Met (c-Met) with ab216330 with ab216330 at 1/50 dilution, followed by a AlexaFluor®488 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution (green). Confocal image showing membranous and cytoplasmic staining in B16-F10 cell line. Tubulin was counterstained using an Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)at 1/200 dilution. The nuclear counterstain was DAPI (Blue).

    Negative control: NIH/3T3 (PMID: 21496277, 8197126, 9888438)

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Product protocols

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